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10 January 2012, Volume 26 Issue 1 Previous Issue    Next Issue
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Genetic Analysis and Gene Mapping of a Short Root Hair Mutant ksrh1 in Rice DING Wona, WU Jing, LUO Lili, QIAN Zhouting, ZHU Shihua 2012, 26(1): 1-4.  DOI: 10.3969/j.issn.10017216.2012.01.001 Abstract ( )   PDF (895KB) ( )   A rice mutant with significantly shorter root hairs was isolated from an EMSinduced mutant library of  indica Kasalath.  The mutant was named as Kasalath short root hair 1 (ksrh1). Except shorter root hairs, there was no significant difference between ksrh1 and its wild type. Genetic analysis showed that the mutant was controlled by a single recessive nuclear gene. To map  the gene  KSRH1, the mutant  ksrh1 and Nipponbare were crossed to generate an F2 population. By using the published SSR markers and some newly designed STS markers,  KSRH1   was mapped to a 67 kb region between the STS markers S3578 and S3584 on the long arm of chromosome 1.

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Resistance to High Salt and  Cold Stress of Transgenic Rice Seedlings with Overexpressed and RNAisilenced OsBTF3 LI Guangxu1, 2, CHEN Huamin1, WU Maosen1, HE Chenyang1,* 2012, 26(1): 5-8.  DOI: 10.3969/j.issn.10017216.2012.01.002 Abstract ( )   PDF (787KB) ( )   To demonstrate the biological function of OsBTF3 gene in rice resistance  or tolerance to salt and cold stress, the gene transcription of OsBTF3 responsive to salt and cold treatments was quantified via realtime PCR assay, and resistance to the stress of transgenic rice seedlings with overexpressed and RNAisilenced OsBTF3 was analyzed. The  results  showed that gene expression of OsBTF3 was significantly decreased in rice plants under salt stress, while lightly reduced under  cold stress. The resistance to salt and cold stress was  enhanced highly  in the seedlings of transgenic lines with overexpressed OsBTF3, while   decreased   in the RNAisilenced OsBTF3 lines. The results revealed that  OsBTF3 might function as a key regulator for abiotic stress resistance in rice.

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Detection of Two Nonstructural Proteins Encoded by Genome Segment S7 of Rice BlackStreaked Dwarf Virus in Infected Rice Plants LU Mingfang1,2, YANG Jian2, ZHANG Hengmu2, CHEN Jianping2,* 2012, 26(1): 9-15.  DOI: 10.3969/j.issn.10017216.2012.01.003 Abstract ( )   PDF (719KB) ( )   The genome segment S7 of Rice blackstreaked dwarf virus (RBSDV) contains two nonoverlapping major open reading frames (ORFs), which encoded two proteins,  p7a and p7b,respectively. Based on the sequence of S7 (EU111804), two pairs of S7specific primers were designed and respectively used to amplify the fragments encoding the p7a and p7b proteins. The expected products were  subcloned into the expression vector pET32a（+） or pSBET and expressed at high level in E. coli BL21(DE3)pLysS or BL21(DE3)pLysE, respectively. An antiserum specifically against p7a or p7b proteins was prepared using the purified expression proteins to immunize mice. The results of Western blot revealed that both p7a and p7b proteins were expressed in infected plants, and p7a protein was more abundant and easily detected than p7b protein. However, both have not been detected in the viral particles, indicating that both proteins encoded by RBSDV S7 were nonstructural proteins.

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Changes in  Small Nucleolar RNA Transcripts in Rice Roots under Osmotic Stress  by Microarray MA Tingchen1,2, CHEN Rongjun3, YU Rongrong1, ZENG Hanlai2, ZHANG Duanpin2,* 2012, 26(1): 16-20.  DOI: 10.3969/j.issn.10017216.2012.01.004 Abstract ( )   PDF (1037KB) ( )   The   expression level  of snoRNA transcripts  was investigated under PEGstimulated  drought stress  by Affymetrix 60 k rice genome array. The expression of rice snoRNA transcripts  was  cultivarspecific under  drought stress. A total of 23 snoRNA transcripts from Aihua 5  and only two snoRNA transcripts from Xiangfengzao 119 were upregulated under the osmotic stress. The snoRNA transcripts   from two cultivars were box C/D type and  presented in five gene clusters  on  the chromosomes for these snoRNA transcripts.  Moreover, an overlap transcript encoding Z274 snoRNA from two cultivars was obtained, but the changed fold of the Z274 snoRNA transcript from Aihua 5 was 1654 fold more than that of Xiangfengzao 119.

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Construction of   Suppression Subtractive Hybridization cDNA Library of Imidaclopridresistant and Imidaclopridsusceptible Brown Planthopper ZHANG Juefeng, HE Yueping, CHEN Jianming*, CHEN Liezhong 2012, 26(1): 21-26.  DOI: 10.3969/j.issn.10017216.2012.01.005 Abstract ( )   PDF (705KB) ( )   The forward and reverse subtracted cDNA libraries of imidaclopridresistant and  imidaclopridsusceptible brown planthopper (Nilaparvata lugens Stl) populations were constructed by  suppression subtractive hybridization (SSH), and the relative genes regulated by imidacloprid were cloned. The results showed that 120 positive clones obtained represented 92 different  single genes, in which 17   shared  higher homology with known protein genes of animals in the GenBank. These single genes involved signal transduction, biotic and abiotic stress, reproduction, and etc.

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Effects of Seedlingraising Fertilizer on Rice Seedlings  and Its Proteomics Analysis PENG Chunrui1, 2,#, SHAO Caihong2, #, PAN Xiaohua1,*, QIAN Yinfei2, QIU Caifei2, XIE Jinshui2 2012, 26(1): 27-33.  DOI: 10.3969/j.issn.10017216.2012.01.006 Abstract ( )   PDF (1152KB) ( )   The differences in  leaf  expressed proteome of early  rice seedlings   with   seedlingraising fertilizer and chemical fertilizer as basal fertilizer  were compared to reveal the mechanism that   seedlingraising fertilizer  application  improved rice seedling quality. The seedlingraising fertilizer could promote tillering and root growing, help form stocky plants, increase chlorophyll content and promote photosynthesis, finally improve  comprehensive quality of seedlings. Proteins collected from rice seedling leaves were analyzed using twodimensional electrophoresis in combination with biomass spectrometry. According to  the protein expression profiles,  the  expression levels  of 18 protein spots altered under the two cultivation conditions. Among the 18 protein spots, 16 proteins were identified, including nine proteins involved in photosynthesis, three proteins in stress resistance, three proteins in protein synthesis and  one  functionunknown protein.

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Discovery of a Germplasm with Large Flag Leaf Angle and Its Genetic Analysis as well as QTL Mapping in japonica Rice HU Wende1,2,#, ZHANG Hong1,#, JIANG Jianhua1, WANG Yingying1, SUN Dayun1, WANG Xiaoshuai1, HONG Delin1,* 2012, 26(1): 34-42.  DOI: 10.3969/j.issn.10017216.2012.01.007 Abstract ( )   PDF (2279KB) ( )   Genetic analysis for the flag leaf angle was conducted using six generations of P1, P2, F1, B1(F1/P1), B2(F2/P2) and F2derived from the cross between 863B(a maintainer line of japonica rice) and A7444(a germplasm with large flag leaf angle). Genotypes and phenotypes of flag leaf angle were investigated in 863B (P1), A7444 (P2) and 141 B1 plants. An SSR genetic linkage map was constructed and QTLs for flag leaf angle were detected. The genetic map containing 79 information loci was constructed,covering a total distance of 441.6 cM, averaging 5.6 cM between two neighboring loci. Results showed that the trait was controlled by two major genes plus polygene with dominance of major genes. Fifteen markers showed highly significant correlations with flag leaf angle based on single marker regression analysis. Two QTLs，  qFLA2 and qFLA8 for flag leaf angle were detected at the intervals of RM300-RM145 and RM6215-RM8265, respectively with WinQTLCart 2.5 and QTL Network 2.0. The positive alleles  at   the two QTLs were both contributed by A7444.

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Fine Mapping of a Major QTL qHd8.1 for Heading Date in Rice ZHOU Yong, CUI Guokun, ZHANG Yanzhou, GUAN Chengran, CHANG Siyuan, GU Minghong, LIANG Guohua* 2012, 26(1): 43-48.  DOI: 10.3969/j.issn.10017216.2012.01.008 Abstract ( )   PDF (668KB) ( )   C23011,  containing a single introgressed segment of chromosome 8, was derived from the CSSL population with  Nipponbare as the donor and Guanglu′ai 4 as the recurrent parent.  In normal longday conditions, its  heading date  was 14.1 d shorter  than that of Nipponbare. Under shortday conditions, however, no obvious difference in heading date between C23011 and Nipponbare was observed. A segregation population derived from a BC4F2 individual with the heterozygous target segment was used for genetic analysis and gene mapping. The ratio of late heading plants to early heading plants showed a 3∶1 frequency distribution，suggesting that the heading date variation was controlled by a single major QTL, which was named qHd8.1.  A physical map   was constructed encompassing the qHd8.1 locus and  the locus was delimited   to a 59.94 kb  interval  between markers S8111 and S849 on BAC clone AP005164 using 1628 individuals with  shorter heading date originated  from the BC4F3 populations. There are seven predicted genes within this region.

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Selection of Rice Restorer Lines with Salinity Tolerance Through Salt Solution  Irrigation over Whole Growth Stage under  Field Conditions PAN Xiaobiao1, HUANG Shanjun1, CHEN Kai2, MENG Lijun2, XU Jianlong2,* 2012, 26(1): 49-54.  DOI: 10.3969/j.issn.10017216.2012.01.009 Abstract ( )   PDF (417KB) ( )   During 2009-2010, salt tolerance(ST) of 49 introgression line populations derived from Minghui 86 were evaluated according to performance of STrelated traits including percentage of survival plants, seed setting rate and grain weight per plant under 0.5% salinization (sea water+fresh water) over the whole growth stage. The results indicated that the populations differed in ST  even derived from the  same subspecies, indica or japonica. The introgression populations originated from japonica parents showed better ST than those originated from indica parents. The four populations with Shennong 265, Y134, Zaoxian 14 and Gayabyeo as donor parents had high percentage  of salttolerant lines, 10.04%, 6.98%, 11.83% and 7.9%, respectively. High reproducibility of  the results in the two years conformed the high reliability. The hybrids between the restorer lines with ST and Xieqingzao A were evaluated for heterosis under  conventional cultivation. Six elite combinations with better performance in panicle number per plant, spikelet number per panicle, 1000grain weight and grain yield per plant were selected from the progenies of Xieqingzao A/salttolerant restore lines under conventional cultivation. Field salt stress over whole growth stage  creat favorable conditions  for breeding of hybrid rice with ST and high yield potential.

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QTL Dissection of Plant Height at Different Growth Stages Under MultiEnvironments in japonica Rice  JIANG Jianhua1, ZHANG Wanxia1, LIU Xiaoli1, LIU Qiangming1, LU Chao1, DANG Xiaojing1, ZHAO Qibing2, HONG Delin1,* 2012, 26(1): 55-64.  DOI: 10.3969/j.issn.10017216.2012.01.010 Abstract ( )   PDF (923KB) ( )   To understand the genetic basis of dynamic development of plant height in japonica rice, the static and dynamic loci for the trait were detected by unconditional and conditional QTL mapping methods using a population of 254 recombinant inbred lines derived from the cross between Xiushui 79 and C Bao. Phenotypic values   were investigated under  three different environments  and analyzed by the mixturelinearmodel. Normal distribution and transgressive segregation in both directions were observed in the RIL populations under three environments. The results indicated that the impact on plant height caused by environment would be reduced with plant growth and development. Five QTLs were detected at maturity stage, and qPH8.3 was unique at this stage. Fifteen unconditional additive QTLs were identified at nine  developmental stages. The  positive alleles of identical additive loci detected at different stages were originated from the same parent and the additive effects were increased with the plant growth. Sixteen conditional additive QTLs and sixteen epistatic QTL pairs involved in plant height were identified at nine dynamic measurement stages. Among them, six additive QTLs were detected at  two stages, while the others were detected only at a  stage. The additive effect was the major genetic effect at the three stages including from sowing to 42 d after transplanting,  from 56 d  after transplanting to 70 d and from 98 d  after transplanting to 112 d. From 42 d after transplanting to 56 d and from 70 d to 84 d, plant height was controlled both by additive and epistatic effects. Whereas epistatic was the major genetic effect from 84 d after transplanting to 98 d. Effect of G×E interaction was small during all developmental stages. Combining unconditional  QTL mapping with  conditional QTL mapping  under multienvironments   is a promising strategy to elucidate  the genetic basis of plant height at different development stages.

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Molecular Prediction of Milling Quality   of  indica  Hybrid Rice Based on Different Analyzing Strategies LING Yinghua, YANG Zhenglin, ZHAO Fangming, SANG Xianchun, LI Yunfeng, HE Guanghua* 2012, 26(1): 65-69.  DOI: 10.3969/j.issn.10017216.2012.01.011 Abstract ( )   PDF (368KB) ( )   Brown rice rate (BRR), milled rice rate (MRR) and head rice rate (HRR) are important traits determining rice milling quality, so the effective prediction of these traits is of great importance to  hybrid rice breeding. Positive loci (PL) and effectincreasing loci (IL) for BRR, MRR and HRR  detected with  174 SSR   markers were used to predict the performance of these three milling quality traits of 81 hybrid combinations  derived from diallel crossing between 9 male sterile lines and 9 restorer lines via twogroup and threegroup methods, respectively. The results indicated that: 1) the correlation coefficients predicted via  the twogroup method with  IL of BRR, MRR  and HRR were 0.553 (P<001), 0.613 (P<001)  and 0.772 (P<0.01), respectively, significantly  higher than those with  PL; 2) with fixed male sterile lines, the correlation coefficients and significant level of three milling quality traits predicted with  IL were much higher than those with fixed restorer lines, suggesting that   male sterile lines exerted  a higher influence on BRR, MRR  and HRR  than restorer lines; 3) with fixed male sterile lines, the predicting correlation coefficients of BRR, MRR, and HRR via threegroup method were 0.466 (P<0.05), 0.564 (P<0.01)  and 0.688 (P<0.01) correspondingly, decreased by 17.7%, 8.0%  and 10.9%   than those via twogroup method, while the coefficients agreed well with each other with fixed restorer lines; 4) to the molecular prediction  of milling quality   in hybrid rice, the efficiency with fixed male sterile lines is statistically higher than that with fixed restorer lines, results  by  twogroup method is better than that by  threegroup method. And among these three milling quality traits, HRR is more easier to predict,  followed by  MRR  and BRR.

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Genetic Diversity  of 47 Weedy Rice with  SRAP Markers LIU Dan, SUN Jian, MA Dianrong*, WANG Jiayu, TANG Liang, MIAO Wei, CHEN Wenfu* 2012, 26(1): 70-76.  DOI: 10.3969/j.issn.10017216.2012.01.012 Abstract ( )   PDF (892KB) ( )   Genetic diversity of 47 weedy rice accessions collected from disasterprone areas were analyzed with SRAP molecular markers to further reveal the source of weedy rice based on the genetic background of weedy rice at the level of open reading frame. Among 845 DNA bands amplified with 110 pairs of primers   590  were polymorphic, 5.4 bands per pair of primers,  accounting for  69.82%.   This implied that  with  a high amplification  efficiency at the gene coding region SRAP molecular marker   was suitable  for  genetic diversity analysis.  ShannonWeaver information index of weedy rice in China was 0.63, the  diversity index (I)  of weedy rice from the north and the south of China were  0.62 and   063. On the basis of genetic distance cluster analysis, significant genetic differentiation was detected between   weedy rice from the north and the south. Cheng′s index demonstrated that   weedy rice  from the south of China could be classified as indica rice and  weedy rice from the south   as japonica rice.

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Effects of Alternate Dryingwetting Irrigation During Grain Filling on Grain Quality and Its Physiological Mechanisms in Rice  LIU Lijun1, LI Hongwei1, ZHAO Buhong2, WANG Zhiqin1, YANG Jianchang1，* 2012, 26(1): 77-84.  DOI: 10.3969/j.issn.10017216.2012.01.013 Abstract ( )   PDF (867KB) ( )   The effect of drywet alternate water treatment during grain filling on grain quality and its physiological mechanisms were investigated with Yangdao 6 (indica) and Wuyujing 3 (japonica) as materials. The results showed milling quality and appearance quality were improved, breakdown values of rice flour were increased and setback values were decreased under alternate  moderate drying  and wetting treatment (MD). The results were reversed under   alternate severe  drying  and wetting  treatment (SD).  The activities of sucrose synthase (SuSase), ADP glucose pyrophosphorylase (ADPGase), soluble starch synthase (SSSase), and Qenzyme in grains increased under MD during grain filling, while  decreased under SD. The enzyme activities were significantly correlated with main indices of grain quality. The concentrations of 1aminocylopropane1carboxylic acid (ACC,biosynthetical precursor of ethylene) in root bleeding, the concentration of ACC and ethylene evolution rate in grains were significantly decreased under MD, while significantly increased under SD. The concentrations of ACC in root bleeding, the concentration of ACC and ethylene evolution rate in grains were significantly or extremely  significantly and   negatively correlated with the activities of SuSase, ADPGase, SSSase and Qenzyme. The shifting concentration of ACC in root  under  alternate dryingwetting   treatment  during grain filling  affected the concentration of ACC and ethylene evolution rate in grains, which influenced the activities of enzymes involved in starch synthesis, and grain quality finally.

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Effects of Rape Straw Manuring on  Soil Fertility   and  Rice  Growth WANG Danying1, PENG Jian1, XU Chunmei1, ZHAO Feng1,2, ZHANG Xiufu1,* 2012, 26(1): 85-91.  DOI: 10.3969/j.issn.10017216.2012.01.014 Abstract ( )   PDF (1051KB) ( )   In order to definite the effects of rape straw manuring on soil fertility and  afterreap  rice growth, four   planting models were designed  in year 2004-2008 in  Fuyang, Zhejiang Province, China, including fallow(CK), Chinese milk vetch(T1), returing burned  rape straw to field after harvest(T2), returning infullblooming rape to field(T3). The results showed that  under  the T3  treatment, the organic content, total N content,  and total P content increased, the bulk density and  soil specific gravity of the soil decreased, improving paddy field fertility, physical traits, soil urease and acid phosphates activities in  afterreap rice growth period. Under T3 afterreap rice growth was improved and the yield of all rice cultivars increased. This attributes to increased number of effective panicles in hybrid rice Nei 2 you 6, Liangyoupeijiu and Xiuyou 5, and to increased number of grains per panicle in  conventional  rice cultivar Xiushui 5. In photosynthesis traits, after returning rape as green manure, rice leaf area index, flag leaf length, width, chlorophyll content, photosynthetic rate and biomass all increased. Results showed returning rape as green manure to paddy field is a very good biofertilization method.

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Effects of LongTerm Green Manure Application on System Productivity and Stress Resistance of Paddy Field in the South of the Yangtze River Valley WANG Li1, WANG Xin1, YU Xichu2, HUANG Qianru2, ZHAO Feng1, ZHANG Weijian1,3,* 2012, 26(1): 92-100.  DOI: 10.3969/j.issn.10017216.2012.01.015 Abstract ( )   PDF (979KB) ( )   The effects of longterm green manure application on the productivity and stress resistance of a double rice cropping system were investigated in  a 28year field experiment with different fertilizing regimes and in a 4year pot experiment of nutrient depletion. The results showed that no significant differences were observed in the mean biomass and grain yield of early rice, late rice and the wholeyear   between the green manure application and the chemical fertilization treatments over the 28year period, whereas green manure application  decreased  the rates of chemical N, P and K fertilizer application by 90.0 kg/hm2, 9.9 kg/hm2 and 72.0 kg/hm2,  respectively. The wholeyear grain yield  followed an increasing trend under  the two green manure application treatments, while a declining trend were found  under  the chemical fertilization treatment. The 4year pot experiment indicated that green manure application could enhance the resistance of the double rice cropping system to nutrient depletion, especially in the plots with moderate green manure amendment. Although there was no significant difference in the productivity of double rice cropping system between the green manure application treatments, the resistance to nutrient depletion was greater at the moderate application level than the high application level.

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Characterization of Leaf Carbon and Nitrogen Assimilation in Different Rice Genotypes at Jointing Stage and Their Relationships with Nitrogen Utilization Efficiency CHENG Jianfeng1, *， DAI Tingbo2， JIANG Haiyan1， PAN Xiaoyun1， CAO Weixing2 2012, 26(1): 101-108.  DOI: 10.3969/j.issn.10017216.2012.01.016 Abstract ( )   PDF (1419KB) ( )   The leaf carbon and nitrogen metabolism characteristics and their relationships with  nitrogen utilization efficiency(NUE) at  jointing stage were investigated with Xiuhui 2 (low NUE), R8312 (medium NUE) and Wuyou 244 (high NUE) as materials  under the lower nitrogen level (58 mg/kg). The results showed that the contents of sucrose and soluble sugar  in the leaves of different rice genotypes    were Wuyou 244 > R8312 > Xiuhui 2, the ratio  of starch content to soluble sugar content and  the ratio of starch  content to sucrose  content  were Xiuhui 2>R8312>Wuyou 244, significantily differed among genotypes. The  starch content and the  ratio of sucrose content to soluble sugar content were not genotypically specific. The  total nitrogen content (TNC), protein nitrogen content (PNC), PNC/TNC, RC(Rubisco content)/PNC  and RC/TNC in the leaves of different rice genotypes exhibited significant differences,  showing an increasing trend of Wuyou 244 < R8312 < Xiuhui 2  for the former two indices and a trend of Xiuhui 2>R8312>Wuyou 244 for the latter  three; RC was not genotypicallyspecific. The contents of chlorophyll and carotenoid, activity of PSⅡ, carboxylation efficiency, activities of carbonic anhydrase, acid invertase and sucrose phosphate synthase in the leaves of Wuyou 244 with high NUE were significantly higher than those of the other genotypes. The activities of nitrate reductase, glutamine synthetase and glutamate synthase and glutamate dehydrogenase in the leaves of Wuyou 244 with high NUE were also significantly higher than those of the other genotypes. Statistical analyses indicated that the leaves of rice genotype with high NUE had the distinguished characteristics of higher soluble sugar content, sucrose content, RC/TNC and RC/PNC, their physiological base were higher   glutamine synthetase activity and primary PSⅡ activity.

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Fluctuation and Migration of  Spring Population of   Small Brown Planthopper (Laodelphax striatellus) on Wheat in Jiangsu and Zhejiang Provinces HE Yuan1 , ZHU Yubo1 , HOU Yangyang1 , YAO Shitong2 , LU Zhijie2 , JIN Zhouhao2 , ZHANG Xiaoxi1 , ZHAI Baoping1,* 2012, 26(1): 109-117.  DOI: 10.3969/j.issn.10017216.2012.01.017 Abstract ( )   PDF (2475KB) ( )   The field surveys for overwintering and spring populations  of the small brown planthopper \[Laodelphax striatellus (Fallén), SBPH\] on wheat and weeds were performed, and the hourly immigration under the light trap was observed in northern Jiangsu Province and Haining, Zhejiang Province. The results illustrated that  SBPH overwintered as 3rd-5th instars (mainly as 4th instar) on wheat, weeds and rice stubble from October to the next March.  The overwintering nymphs finished their dormancy in March and emerged during March to April. The first generation nymphs were found in wheat during April to May.  From the end of May to the midJune, mass of the macropterous adults of SBPH emigrated in wheat fields, then immigrated into rice fields and weeds after a short or long journey. The emigrated and immigrated population could be distinguished by the status of ovarian development. The hourly collected light trap catches revealed that SBPH did migrate regularly for several hours after taking off at dawn and dusk, even an all night longdistance flight. SBPH collected by the  light trap showed a bimodal takeoff peak and they would immigrate at every hour during all night, but mainly before midnight. The source regions of the immigrants were tracked by trajectory analysis using NOAA HYSPLIT model, which proved that the SBPH immigrants might come from the middle and north Jiangsu Province, southern Anhui Province, southern Zhejiang Province, northeastern Jiangxi Province and northwestern Fujian Province.

Short Communications

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Effect of Two Biotypes of Jiangdu Weedy Rice  at Various Transplanting Densities on  Growth of Cultivated Rice and Their Genetic  Relationship SUN Xingqiang1，2, ZHOU Yongjun1, LU Yongliang1, CHEN Lijuan2, YU Liuqing1,* 2012, 26(1): 118-122.  DOI: 10.3969/j.issn.10017216.2012.01.018 Abstract ( )   PDF (499KB) ( )   To explore biotypes, genetic characteristics of weedy rice in Jiangdu, Jiangsu Province, China, and  their genetic  effect on yield of cultivated rice,  morphological characteristics were detected and cluster analysis was conducted based on SSR markers. The results showed that the weedy rice materials can be divided into two biotypes: One is dwarf, including JDWRA and JDWRC, the other one is tall, including JDWRB; the two biotypes of weedy rice belong to indica, JDWRA was most related to JDWRC, the dwarf type of weedy rice was distantly related to the tall type, and  closely  related to indica  male sterile line Zhenshan 97A. The competition test between weedy rice and cultivated rice indicated that the dwarf type of weedy rice more significantly influenced on yield factors of cultivated rice.

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Design of Specific Primer for Trichogramma japonicum Based on rDNAITS2 and Application of Diagnostic Primers in Identification of Four Trichogramma Species GUO Zhen1, RUAN Changchun1, ZANG Liansheng1,*, ZHANG Fan2,*, JIN Fengyun3 2012, 26(1): 123-126.  DOI: 10.3969/j.issn.10017216.2012.01.019 Abstract ( )   PDF (659KB) ( )   The speciesspecific primer for  Trichogramma japonicum Ashmead was designed based on its rDNAITS2 sequence with Primer Premier 5.0. With improvement of the conditions of PCR, a 256bp fragment of T. japonicum could be amplified repeatedly with the specificiallydesigned primer. PCR amplifications of Trichogramma samples were carried out with three pairs of diagnostic primers for T. dendrolimi, T. ostriniae, T. chilonis  reported previously, and the designed primer for T. japonicum. The results showed that the specific fragment of each Trichogramma species could be amplified with corresponding specific primers, respectively. Furthermore, the results of molecular identification were consistent with those based on morphological identification of male genitalia.