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10 November 2011, Volume 25 Issue 6 Previous Issue    Next Issue
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Research Papers

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Primary Function Analysis of a  Pyruvate Decarboxylase Gene, OsPDC3, in Rice CHEN Bo, HAN Bin* 2011, 25(6): 567-574.  DOI: 10.3969/j.issn.10017216.2011.06.001 Abstract ( )   PDF (1757KB) ( )   There are high aerobic fermentation rates in plant pollen. As  a key enzyme of ethanolic fermentation pathway, pyruvate decarboxylase (PDC) is involved in  energy production and biosynthesis in pollen,  playing  an important role in the pollen germination process.OsPDC3  gene function was investigated by the reverse genetics methods. The gene is  a single exon gene encoding a protein that shares      77%-82%   sequence identity  with other rice PDC proteins. Expression pattern analysis showed  that the OsPDC3  specifically expressed in pollen, and  the pollen specific expression activity of the promoter of  OsPDC3 was confirmed by GUS staining. Overexpression of OsPDC3 in transgenic plants improved  the    activity of PDC in leaves, indicating functional enzyme activity of OsPDC  in vivo.  Antisense suppression downregulated  the expression of OsPDC3 in pollen, but the pollen germination rate in vitro  remained  unchangable, which might attribute to  the redundancy among the PDC genes.

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Effects of Suppressing  OsCRY1a Gene Expression on Rice Agronomic Traits LI Yu1,2，ZHUANG Weijian1,3,*，WANG Naiyuan2，HONG Guoqin1，DAI Fei3 2011, 25(6): 575-579.  DOI: 10.3969/j.issn.10017216.2011.06.002 Abstract ( )   PDF (608KB) ( )   Using primers designed according to the published sequence of   OsCRY1a gene，  part of the gene fragment  was obtained by PCR and  a RNAi expression vector  was constructed. The vector was then introduced into rice,  leading to the downregulation  of the expression level and lossoffunction of the gene. Based on the performance of the transgenic plants, the functions of OsCRY1a was analyzed and deduced. The results indicated that suppressing the expression of OsCRY1a    retarded  flowering for 16 d  with    the plant height and grain length significantly increased   while  other important agronomic traits    remained unchanged  apparently.

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Excision of Marker Genes in Transgenic Rice at Transformed Cell Level using  Cre/loxP System ZHAO Yan1,2,*, ZHANG Xiaoli2, GUO Longbiao1， QIAN Qian1,* 2011, 25(6): 580-586.  DOI: 10.3969/j.issn.10017216.2011.06.003 Abstract ( )   PDF (1743KB) ( )   The feasibility and the efficiency of excision of marker genes in transgenic rice were studied  at transformed cell level  by  using the  Cre/loxP system. The vector pNCG including estrogeninduced Cre/loxP marker gene excision system was introduced to rice cell via Agrobacteriummediated transformation. After the resistant calli  were screened out under G418 selection， the Cre gene expression was induced to excise the marker gene by adding 25 μmol/L estrogen to the medium at different stages of tissue culture. The integration of marker gene nptⅡ, recombinase gene Cre and target gene gusA in regenerated transgenic rice plants was analyzed  by PCR. The plantlet  of gusA（+）/nptⅡ（-）/Cre（-） in PCR test were calculated as transgenic plants  with  excision  successful. Results showed that marker gene sequences could be excised successfully by estrogen induction at three tissue culture stages including before predifferentiation, predifferentiation and differentiation. The frequency of marker gene excision ranged from 6.82% to 46.43%. When transformed calli were treated by estrogen in liquid medium for 3 d before predifferentiation，the efficiency of marker gene excision in T0 transgenic rice plants was as high as 173.33%, mainly because the estrogen treatment improved the green plant regeneration frequency of calli. When estrogen was directly added to differentiation medium for induction treatment, the frequency of marker gene excision in T0 transgenic rice plants was 46.43% with a higher efficiency of marker gene excision （144.44%）. The  results confirmed  that marker gene sequences in transgenic rice plants could be excised efficiently and quickly by using  the Cre/loxP system under estrogeninduced treatment   at resistant calli level.

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Morphological Structure and Genetic Mapping of a New Leaf Color Mutant  in Rice LI Yuhong1,2, WANG Baohe2, DAI Zhengyuan2, LI Aihong2, LIU Guangqing2, ZUO Shimin1, ZHANG Hongxi1,2,*,PAN Xuebiao1，* 2011, 25(6): 587-593.  DOI: 10.3969/j.issn.10017216.2011.06.004 Abstract ( )   PDF (2310KB) ( )   A natural leaf color mutant  gwgl in rice, named Baihuaidao 7,  showed  a typical phenotype of greenwhitegreen leaf at different stages. The mutant character only arose under   signal stress induced by mechanical  damage  such as transplantation.  The   leaf color, chlorophyll a  content and ultrastructure of chloroplast of the  mutant were almost the same with the wild typle at  the seedling stage. After the leaf changed white, total chlorophyll, chlorophyll a, chlorophyll b and carotenoid  contents of the  mutant were evidently decreased, the thylakoid  of the mutant were  gradually degraded, and the chloroplast grana lamella and grana were decreased. At maturity, the plant of the mutant was etiolated  and dwarfed. The genetic analysis indicated that the leaf mutant was controlled by a recessive nuclear gene. Genetic mapping of the mutant gene was conducted by using  the F2 mapping population of Baihuaidao 7/Jiangxi 1587. The results suggested that the mutant gene was mapped on rice chromosome 11 between InDel markers L59.27 and L64.811. The physical distances between the  two InDel markers were about 740.5 kb. The mutant gene was considered to be a new leaf gene in rice and designated tentatively as GWGL，which laid a good foundation for finemapping, cloning and functional analysis of the mutant genes.

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QTL Analysis for Fertility of  IndicaJaponica  Hybrids in Rice LIN Lihui1, WANG Aimei1, ZHOU Yuanchang1, GUAN Huazhong1, JIANG Yunlin1, WU Jianmei1, WU Weiren2，* 2011, 25(6): 594-598.  DOI: 10.3969/j.issn.10017216.2011.06.005 Abstract ( )   PDF (835KB) ( )   An F2 population consisting of 176 individuals was developed from a cross between an  indica  maintainer line Zhenshan 97B and a japonica   maintainer line Liao 91B and a genetic map containing 123 SSR markers was constructed based on the population. Pollen fertility and spikelet fertility were investigated in three seasons. Three maineffect (additive effect and dominant  effect) quantitative trait loci (QTLs) for pollen fertility (qPF3,  qPF5 and qPF6) and four maineffect QTLs for spikelet fertility (qSF3, qSF5, qSF6 and qSF8) were detected. Among them, qPF6 and qSF6,   and qPF5 and qSF5, important QTLs for rice fertility,  were mapped on  the same locations.   In addition, three pairs of QTL interaction for pollen fertility and four pairs for spikelet fertility were detected, suggesting that epistatic effects play important roles in the fertility of indicajaponica hybrids.

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Breeding and Identification of InsectResistant Rice with Two Insecticidal Genes, sbk and sck LI Cong1,#, ZHANG Qijun2,#, LIU Shaokui1, LAI Dong1, QI Qingming2, LU Chuangen2,* 2011, 25(6): 599-604.  DOI: 10.3969/j.issn.10017216.2011.06.006 Abstract ( )   PDF (935KB) ( )   The plasmid of pCDMARUBAHyg, which contained two insectresistant genes, sbk\[modified from Cry1A(c)\]  and sck(modified from CpTI), was transformed into an Agrobacterium EHA105 for infection of the calli of   a super japonica rice   Nanjing 45. Plants regenerated from the transformation were subjected to all kinds of gene verifications and selection. Using PCR detection with the primers of sbk and  sck genes, 42 positive transgenic plants that were markerfree and contained the two target genes were selected from 97 regenerated plants. Results of Southernblotting indicated that 23, 11, 5, 2 and 1 plants had one, two, three, four and five copies of the transformed genes, respectively.  Analyses of  RTPCR and  Bt gene testing paper showed that  28 T3 generation plants derived  from four   transgenic plants having a single copy  were insectresistant.  Feeding experiment with rice stem borer revealed  that the insect resistance was greatly increased  with nymph mortality ranging from 94% to 100%. In addition, among the transgenic plants, three T3 transgenic plants  had many desirable characteristics for production. The mechanism of insectresistance of Bt  gene and its application in rice transgenic research were also briefly discussed.

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Effects of  OxygenIncreasing Patterns in Paddy  Fields on Rice GrainFilling  ZHAO Feng1, 2, ZHANG Weijian1, ZHANG Xiufu2,*, WANG Danying2, XU Chunmei2 2011, 25(6): 605-612.  DOI: 10.3969/j.issn.10017216.2011.06.007 Abstract ( )   PDF (816KB) ( )   The effects of oxygenincreasing patterns in paddy  fields on rice yield formation and grainfilling dynamics were investigated in a twoyear field experiment (2008-2009) under three oxygenincreasing patterns, including application  of urea peroxide as topdressing (T1), application of calcium peroxide as topdressing (T2) and drywet alternate irrigation (T3), with continuous submerged irrigation  as control (CK). The Richards equation was used to simulate  grain filling processes of two rice genotypes (Guodao 1 and Xiushui 09) under different oxygenincreasing patterns. Compared with the control (CK), the yields of Guodao 1 (indica)  under T1, T2 and T3 were increased by 11.6%, 8.5% and  13.6% in 2008 and 16.8%,  14.4% and  23.0%  in 2009; Similarly, the yields of Xiushui 09 (japonica)under T1, T2 and T3 were increased by 6.6%, 9.2% and 9.4%  in 2008， and 14.6%， 17.2% and 17.4% in 2009,   respectively. Rice grainfilling process fitted well with the Richards equation(R2>0.989), and the main effects of oxygenincreasing patterns on rice grainfilling were as follows: 1) Maximum grain weight of  inferior and superior grains  were both increased    with smaller difference in grain weight between the two genotypes   and      improved grainfilling synchronism; 2) Inferior grains of the two rice genotypes had a full grain filling process. The  oxygenincreasing  patterns extended the grainfilling  duration of   inferior grains of  Guodao 1,  and  increased the  grainfilling rate of inferior grains  of Xiushui 09. 3) Full grainfilling of the inferior   grains was mainly  contributed  to higher seed setting rate and yield of the genotypes under the  increasingoxygen  patterns in paddy fields .

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Effects of Drought Stress on  Fertility of   NearIsogenic Indica Rice Lines at Flowering Stage FU Guanfu, TAO Longxing* , SONG Jian, XIONG Jie, WANG Xi* 2011, 25(6): 613-620.  DOI: 10.3969/j.issn.10017216.2011.06.008 Abstract ( )   PDF (716KB) ( )   The relationships between spikelet fertility and agronomic  traits or physiological characteristics  related to drought resistance were analyzed with 27 nearisogenic lines and their recurrent parent Zhenfu 802 (Oryza sativa L. subsp. indica) as materials under 15 d drought stress since flowering of main culm. The results presented that  drought stress at the flowering stage not only decreased the plant height, leaf area and plant dry weight, but also significantly decreased the seedsetting rate. Drought resistance of the tested nearisogenic lines has no relationship with the seedsetting rate under wellwatered conditions. According to the drought stress index (DSI), the recurrent parent Zhefu 802 was classed  as drought sensitive variety, and the 27 nearisogenic lines could be divided into drought resistant lines (DSI<0.30), semidrought resistant lines (0.31<DSI<0.50), semidrought sensitive lines (0.50<DSI<0.80) and drought sensitive lines (DSI>0.80). Furthermore, the nearisogenic lines with classic drought resistant morphological traits, such as curl and droopy leaves, failed to show higher drought resistance, whereas those with  yellowish green leaf or golden hull  showed  higher drought resistance.

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Root Characteristics of Broadcasted  Rice Seedlings During Seedling Standing and Its Effect on   Plant Growth GUO Baowei1, ZHANG Chunhua1, CHEN Houcun2, ZHANG Hongcheng1,*, ZHOU Xingtao1, ZHANG Jun1,LI Jie1, CHEN Jingdu1, XU Ke1, WEI Haiyan1, DAI Qigen1, HUO Zhongyang1, XING Lin1, ZHU Congcong1 2011, 25(6): 621-628.  DOI: 10.3969/j.issn.10017216.2011.06.009 Abstract ( )   PDF (848KB) ( )   The root growth characteristics of broadcasted rice seedlings and the effects of broadcasting on plant growth were systematically studied   under the  treatments of  upright seedlings with soil (USWS), leaning seedlings with soil (LESWS),  lying seedlings with soil (LASWS), leaning  seedlings without soil (LASWTS) and   upright seedlings without soil (USWTS).  USWS and LESWS recovered earlier with a smaller dead root ratio, and the length and the dry weight of  roots were both  higher than those of LASWS and LESWTS whose seedling standing speed was extremely and  significantly correlated with the root growth.  USWS and LESWS,  chara`cterized by earlier  tillering  and  higher   productive  tiller  rates, maintained  stronger root activity at later stages and optimum rootshoot ratio throughout  the whole growth period,  displaying  significantly higher nitrogen absorption rates, leaf chlorophyll content and root   depths than LASWS and LASWTS. Considering   earlier standing and recovery, significantly better root growth and fairly stronger growth capacity  of USWS and LESWS, it is reasonable to seek high yielding and super high yielding of broadcasted rice by means of increasing the rate of upright seedlings with soil, reducing  lying  seedlings and improving seedling standing by promoting root  growth.

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“Double Late” Cultivation Model  for Rice and Wheat and Its Comprehensive Evaluation on Annual Productivity GONG Jinlong1 , ZHANG Hongcheng1, * , CHANG Yong1 , HU Yajie1 , LONG Houyuan1 , DAI Qigen1 , HUO Zhongyang1 , XU Ke1 , WEI Haiyan1 , LI Dejian2 , SHA Anqin2 , ZHOU Youyan2 , LUO Xuechao2 2011, 25(6): 629-638.  DOI: 10.3969/j.issn.10017216.2011.06.010 Abstract ( )   PDF (873KB) ( )   Effects of “double late” (latematurity rice and latesowing wheat) cultivation model on annual productivity of rice and wheat were  systematically studied in a field experiment with four cultivation model treatments. Results showed that  with the crop   rotation being delayed, annual grain yield of rice and wheat   increased. Grain yield of rice increased, whereas the  grain yield of wheat increased firstly and then decreased with  the highest yield under  latematurity  medium  japonica  rice+wheat model (LMW). Moreover,  prolonging rice growth duration and developing large panicles improved   annual grain yield of rice and wheat. With maturity being prolonged, productivity with respect to the key inputs of rice increased. But for wheat,    productivity  per unit of seed decreased  and others increased firstly and then decreased  with the best  performance  under LMW model. Appropriate sowing date helped  improve productivity with respect to  key inputs, while  appropriately delayed  crop   rotation was awarding to annual economic productivity of rice and wheat.  Additionally, the concept of “double late” cultivation model and its regulation approaches were discussed.

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Physiological Responses of Weedy Rice to Cold Stress at Seedling Stage in Liaoning Province， China  MIAO Wei, WANG Guojiao, MA Dianrong*, WANG Jiayu， XU Zhengjin， CHEN Wenfu* 2011, 25(6): 639-644.  DOI: 10.3969/j.issn.10017216.2011.06.011 Abstract ( )   PDF (710KB) ( )   Physiological responses of weedy rice to cold  stress  were investigated with weedy rice WR0345 and WR0326 as materials and Lijiangxintuanheigu as   control. The fluctuations of physiological indexes of leaves and roots of  weedy rice and cultivated rice at 5 ℃ in  an artificial climate chamber were investigated. The physiological indexes of leaves of weedy rice and cultivated rice followed a similar trend except the contents of chlorophyll and carotenoids under cold stress. The change of thiobarbituric acid reactive substances(TBARS) and proline contents of weedy rice differed from cultivated rice, but it is otherwise for the change of electrolyte leakage and soluble sugar content   in roots. Photosynthetic system   in weedy rice WR0345 and Lijiangxintuanheigu  was less damaged under  chilling stress. Due to less  injury and membrane lipid peroxidation, the cell membrane of weedy rice WR03 45 remained intact. The  soluble sugar and proline contents of   WR0345 were higher than those of   Lijiangxintuanheigu  under chilling stress. It indicated that weedy rice WR0345  was less injured under  cold stress because of   higher cold tolerance at the seedling stage .

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Effects of N, P, K Fertilizer Application on Grain Yield, Quality, Nutrient Uptake  and  Utilization of Rice WANG Weini1, LU Jianwei1, *, HE Yuqing2, LI Xiaokun1, LI Hui1 2011, 25(6): 645-653.  DOI: 10.3969/j.issn.10017216.2011.06.012 Abstract ( )   PDF (949KB) ( )   Based on the “3414” fertilizer experimental design,  the effects of N, P and K fertilizer application on grain yield, grain quality as well as nutrient uptake and utilization of rice  were researched     to  elucidate  the interactive effects among N, P and K in a field experiment   with four levels of nitrogen (N), phosphorus (P) and potassium (K) fertilizers. The results showed that the application of N, P and K significantly increased grain yield, and  the highest yield was found under the joint application of N, P and K fertilizer. The application of fertilizer also improved rice  quality  markedly. For example, head rice rate was increased and chalkiness degree, lengthwidth ratio of grain and amylose content of milled rice were decreased due to the application of N, P and K. And, the application of N and P increased protein content of brown rice and decreased green rice rate. The application of N, P and K fertilizer increased total nutrient (show as N, P2O5, K2O) accumulation and nutrient absorption per 100kg seeds, but reduced nutrient dry matter production efficiency and nutrient grain production efficiency. Meanwhile, fertilizer recovery efficiency, agronomic efficiency and physiological efficiency always declined with the increasing  N, P and K application rate. Grain yield was significantly influenced by the interaction between N and P, N and K, P and K, respectively, and the interactions were synergistic when fertilizers application rates were at lower or moderate levels, otherwise they were antagonistic. The most significant interactive effect on total N, P2O5 and K2O accumulation was between N and K, N and P, P and K, respectively. It is concluded that the influence of fertilizer on rice growth was various, which means that fertilizers application rate and ratio should be established with an integrated consideration of several factors including grain yield, grain quality and nutrient uptake, utilization efficiency.

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Effects of Rice Black Streaked Dwarf Virus on   Ecological Fitness of NonVector Planthopper, Sogatella fucifera  HE Xiaochan1,2, XU Hongxing2, ZHENG Xusong2, YANG Yajun2, GAO Guangchun2, PAN Jianhong2, LU Qiang3, LU Zongxian2,* 2011, 25(6): 654-658.  DOI: 10.3969/j.issn.10017216.2011.06.013 Abstract ( )   PDF (573KB) ( )   Rice black streak dwarf virus (RBSDV) transmitted by the small brown planthopper (SBPH), Laodelphax striatellus (Fallen), causes rice yield loss in east China. The whitebacked planthopper (WBPH), Sogatella fucifera (Horváth), a nonvector of RBSDV, usually shares the host rice plants with SBPH. We evaluated the effects of RBSDVinfested rice plants on the ecological fitness and   relevant defense and detoxification enzymes of WBPH in laboratory for exploring the relationship between RBSDV and the nonvector insect. The results showed that the  nymph survival rate, sex ratio, female adult weight and fecundity, and egg hatchability of WBPH fed on RBSDVinfested rice plants did not markedly differ from those on healthy plants, whereas  the adult longevity and egg duration were significantly shortened. Furthermore,   the activities of defense enzymes (CAT, SOD and POD) and detoxification enzymes (AchE，GST and CAE)  were significantly enhanced in WBPH adults fed on infected plants. Results implied that infestation by RBSDV improved  the ecological fitness of the  nonvector planthopper.

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Effects of Host Plants on  Activities of Detoxification and Protective Enzymes in Three Rice Planthoppers LIU Yukun, WANG Weixia, FU Qiang*, LAI Fengxiang, LUO Ju 2011, 25(6): 659-666.  DOI: 10.3969/j.issn.10017216.2011.06.014 Abstract ( )   PDF (2238KB) ( )   The effects of host plants on   survival and activities of detoxification   and protective enzymes of three rice planthoppers (Nilaparvata lugens, Sogatella furcifera  and Laodelphax striatellus) were investigated with five host plants including four  rice varieties \[TN1, IR42, Rathu Heenati (RHT) and Wuyujing 3\] and one weed barnyardgrass. The survival rate of the three planthoppers significantly varied with host plants. N.lugens had the highest survival rate on TN1, followed by on  IR42 and Wuyujing 3, and  on RHT and barnyardgrass. However, S. furcifera and L. striatellus had significantly higher survival rate on TN1 as well as on Wuyujing 3 and barnyardgrass, and substantially lower survival rate  on RHT and IR42. Among  the three planthoppers, the activities of glutathione Stransferase  and  microsomal mixedfunction oxidases at  the adult stage, and  the activities   of  carboxylesterase, superoxide dismutase and peroxidase  at both adult and nymph stages, significantly differed on various host plants,  whereas  the activities  of  glutathione Stransferase  and microsomal mixedfunction oxidases   were similar at the nymph stage. The influence of host plants on the  activities of these enzymes were also planthopperrelated, with L. striatellus   most affected,  N.lugens the  least affected, and S. furcifera moderately affected, which was in accordance  with the host range of   these planthoppers. However,    it was also found that, even if the planthoppers   fed  on plants with similar host fitness,  the enzyme activities  varied  among planthopper species, or even between the adult and nymph stages within a planthopper species. It is suggested that relations between the host plant fitness and the  activities of detoxification and protective enzymes differed with species and developmental stage of planthoppers, and also with  the type of host plants.

Experimental Techniques

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Effect of Fixation and Staining Methods on Observation of  Endosperm Cell   Structure in Rice LIU Zhi1, WANG Lingling1, ZHOU Weidong2, CHEN Yifang2, WANG Zhong1,* 2011, 25(6): 667-671.  DOI: 10.3969/j.issn.10017216.2011.06.015 Abstract ( )   PDF (3727KB) ( )   The caryopses of  rice cv. Yangdao 6    were harvested at 3 to 10 days after flowering and prepared for the  conventional glutaraldehydeosmium tetroxide (GAOsO4) fixation and for the potassium permanganate (KMnO4) fixation, respectively. After being embedded with Spurr resin, the sections were made and  stained by ammonium methylbenzene blue staining(AMB), periodic acid schiff(PAS), multicolor dye and    restained by  PASAMB, respectively. The different  effects of the two fixation and four stained methods   were observed and compared. The results revealed that better semithin sections could be obtained by GAOsO4 fixation. The ultrathin sections by GAOsO4 fixation presented a clearer cell structure, but the endomembrane structure was not clear enough. For the   potassium permanganate fixation  method, the endomembrane structure was clear, while polysaccharide and protein were in poor colour. It is recommended  that selecting proper fixation and staining methods should be based on the observation of the contents in the experiments.

Short Communications

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Application  of Capillary Electrophoresis Detection   with Fluorescent SSR Markers in Rice DNA Fingerprint Identification CHENG Benyi, XIA Junhui, GONG Junyi, YANG Shihua* 2011, 25(6): 672-676.  DOI: 10.3969/j.issn.10017216.2011.06.016 Abstract ( )   PDF (795KB) ( )   With  16 hybrid rice male sterile lines, restorer lines and combinations as materials, a capillary electrophoresis detection method with fluorescent SSR markers in rice was primarily developed. The accurate size of DNA fragment could be detected by  the capillary electrophoresis detection method with fluorescent  SSR markers. The capillary electrophoresis detection has the advantages of data accuracy and efficiency compared  with  polyacrylamide gel electrophoresis detection. It provides a   feasible and reliable method for rice DNA fingerprint identification.

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Genetic Analysis and Gene Mapping of a New SemiDwarf and Small Panicle Mutant in Rice WANG Qing1,2,3, WANG Dekai2,* , TAO Yuezhi1,2,* 2011, 25(6): 677-680.  DOI: 10.3969/j.issn.10017216.2011.06.017 Abstract ( )   PDF (909KB) ( )   A  semidwarf and small panicle mutant was obtained    from  a japonica  rice variety Zhonghua 11 through TDNA transformation. The mutant, characterized by  semidwarf  plant,  abnormal small  and semienclosed  panicle, and poor growth potential   was designated as sdsp2(semidwarf and small panicle 2). Genetic analysis indicated that the mutant was controlled by a single recessive gene. A genetic linkage map was constructed using an  F2 mapping population derived from a cross between  the sdsp2  mutant and an  indica  variety Longtefu B on chromosome 6. The gene was finally located on a 116kb region between the STS markers RH632 and RH640，where 14 ORFs were predicted based on Rice Genome Annotation．

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QTL Analysis of  Cold Tolerance  in Japonica Rice Using  Recombinant Inbred Lines  from  02428×02428c at Booting Stage  XIE Yongwu1,2,3 , YANG Shuming1,*, ZENG Yawen1,*, YANG Tao1, PU Xiaoying1, DU Juan1, ZHAO Chunyan2, ZHANG Mingxi4 2011, 25(6): 681-684.  DOI: 10.3969/j.issn.10017216.2011.06.018 Abstract ( )   PDF (726KB) ( )   A coldsensitive  japonica  rice variety 02428  and a coldresistant  rice variety 02428c  were used as parents to produce an F5:6 population composed of 336 recombinant inbred lines.  Numbers of  filled grains, unfilled grains, total grains and seedsetting rate  were measured as phenotypic values  for  evaluating cold tolerance at the booting stage.   A genetic linkage map  was  constructed with 486 SSR markers for QTL mapping. The markers  RM6092, RM6702, RM5954, RM1095, RM1183, RM7643 and RM3411 were linked to the cold tolerance at the booting stage. Three  QTLs controlling cold tolerance at the booting stage were identified on the chromosome 1 with  QTLMapper 1.6.