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    研究快报
    Screening and Preliminary Study on the Chemical Lethal Mutant in Rice Photoperiod Temperature Sensitive Male Sterile Line
    Zhang Jiwen,Wu Xiaozhi
    1999, 13(2): 65-68 . 
    Abstract ( )   PDF (167KB) ( )  
    研究报告
    Incorporation of the Rice Blast Durable Resistance into Hybrid Rice
    Zhou Shaochuan,Zhu Xiaoyuan,Jiang Yanfang,Yang Qiyun,Ke Wei,Miao Ruowei,Wu Shangzhong
    1999, 13(2): 69-72 . 
    Abstract ( )   PDF (117KB) ( )  
    Hybrid rice combinations made by seven blast resistant cultivars including three durably resistant cultivars and four popular sterile lines were studied. The qualitative resistances, quantitative resistances and field resistances in different blast conductive areas were evaluated and compared with the resistant check IR36 and susceptible check Guanglu'ai 4 respectively. Results indicated that the combinations with durably resistant donor showed the same durably resistant characteristics as correspondently durably resistant cultivars. These results suggested that durable resistance to rice blast could be incorporated into hybrid rice.
    Genetic Analysis on Cold Tolerance Characteristics of Yunnan Rice Landrace ( Oryza sativa L.) Kunmingxiaobaigu
    Dai Luyuan,Ye Changrong,Xu Furong,Zeng Yawen,Liang Bin,Wen Guosong
    1999, 13(2): 73-76 . 
    Abstract ( )   PDF (172KB) ( )  
    Seven characteristics, i.e. plant height, panicle length, length of panicle neck, days till heading, total grains per panicle, filled grain rate, special filled grain rate, were genetically analysed under low temperature treatment by using Kunmingxiaobaigu, a Yunnan rice landrace, and Towada, a japonica variety cultivated widely in Japan, and their F1, F2 and B2F1 populations. The results showed that filled grain rate, and special filled grain rate (filled grain rate of nine spikelets from the 3rd to 5th spikelet of three primary rachis branches at the top of each panicle) were the two most sensitive characteristics, could be used as indicators of cold tolerance evaluation at booting stage. Rates of variance of plant height and panicle length at low temperature treatment also could be used as indicators of cold tolerance evaluation. Genetic analysis results showed that there is one or two loci of major gene manipulating the grain fertility. The rates of characteristic approaching to that of recurrent parent by backcrossing are dependent on characteristic itself.
    Observation on Micromorphology of Lemma Surface in Three Wild Oryza Species and Their Hybrids with Cultivated Species
    Huang Yanlan,Zhang Naiqun,He Guangcun,Shu Lihui,Liao Lanjie,Zhu Lili
    1999, 13(2): 77-80 . 
    Abstract ( )   PDF (685KB) ( )  
    The micromorphology of lemma in species of Oryza sativa subsp. indica, O. rufipogon, O. officinalis, O. meyeriana, and the hybrids of wild rice species with cultivated rice were compared and studied with scanning electronic microscope. The distinguished difference between species was observed and the characters of papillae of the three wild rice species were described. For the hybrids of cultivated rice with wild rice, the micromorphology of lemma was influenced by the parents. The trait of column width and the distance between papillae unit came from the wild rice parent; the distance between peaks and peak numbers took after the cultivated rice; lastly, the density of papillae was superior to both parents.
    A Core Collection of Zhejiang Traditional Indica Rice Germplasm
    Wei Xinghua,Yan Qichuan,Ying Cunshan,Zhang Lihua,Zhang Linping
    1999, 13(2): 81-85 . 
    Abstract ( )   PDF (177KB) ( )  
    Effect of Rice Seedling Raising Conditions on Rice Seedling Growth
    Zhang Yongtai,Wu Huai,Wang Zhong,Xiong Fei,Xie Yunfeng,Li Aimin
    1999, 13(2): 86-90 . 
    Abstract ( )   PDF (475KB) ( )  
    Physiological traits and morphological characters of rice seedling under different rice seedling raising conditions, and their effects on rice growth after transplanting were studied by using hybrid rice Shanyou 63. Compared with rice seedling under water raising conditions, rice seedlings under dry raising conditions were shorter and stronger, in which much starch was accumulated, catalase activity was stronger, and chlorophyll content was higher. Rice plants from dry raising seedlings grew roots quickly after transplanting and had more tillers and effective panicles, resulting in the higher yield of grains. Compared with normal dry raising seedlings, rice seedlings that under dry raising conditions at earlier stage and much watered and less lighted at the late stage showed lower starch content and catalase activity and the yield of grains had no significant difference with water raising seedlings.
    Leaf Source Capacity and Photosynthetic Indexes in Different Types of Rice
    Cao Shuqing,Zhai Huqu,Zhang Hongsheng,Zhang Rongxian
    1999, 13(2): 91-94 . 
    Abstract ( )   PDF (175KB) ( )  
    Copper Enhances Plant Regeneration in Callus Culture of Rice (in English)
    Yang Yuesheng,Jian Yuyu,Zheng Yindong
    1999, 13(2): 95-98 . 
    Abstract ( )   PDF (172KB) ( )  
    Experiment on microelements found that copper was the only element responsible for the pronounced effect of enhancement on plant regeneration in rice callus culture. By supplement of copper to the medium, the plant regeneration frequency, the number and the weight of the regenerated plants were evidently increased; the effective concentration range of copper to be used is a broad one. When surveying calli of other varieties using copper at 5 μmol/L, eight out of 15 showed positive response above significant level. Results of the present experiments indicate that raising copper concentration of the medium is a simple and effective method for the enhancement of plant regeneration in rice callus culture.
    Effects of Low Temperature on Photochemical Function and Levels of Thylakoid Proteins of Chloroplasts in Rice Seedlings
    He Junxian,Zeng Yan,Yi Jing,Liang Houguo
    1999, 13(2): 99-103 . 
    Abstract ( )   PDF (221KB) ( )  
    Changes in chloroplast electron transport activity and the levels of thylakoid membrane proteins of rice leaves (Oryza sativa L. var. Saituo) during chilling stress were studied . The results indicated that treating rice seedlings at 4℃ and a light condition of 35 μmol/(m 2·s) for one, two and three days, caused marked and progressive decrease in chloroplast electron transport activity through the whole chain(H 2O→MV), PSⅡ(H 2O→DCBQ) and PSⅠ(DCIP→MV), respectively. The scales of decrease were whole chain >PSⅡ>PSⅠ. The SDS PAGE of thylakoid membranes showed that the contents of most thylakoid polypeptides decreased markedly during the stress, suggesting that the decrease of chloroplast photochemical function may be a result of the destruction of major functional proteins involved. Furthermore, the mechanisms that low temperature may inhibit PSⅡ (photosystem Ⅱ) activity in rice thylakoids were investigatea. The results of determination on DCIP photoreduction activity of PSⅡ, coupled with those from SDS PAGE of thylakoid membranes, indicated that chilling not only damaged oxidative side of PSⅡ, but also affected its reaction center. However, it seemed that only the damage at the oxidative side was responsible for the decrease of PSⅡ activity.
    Emergence Trend and Number Fluctuation of Weeds in Fields of Wet Direct Seeded Rice
    Wang Qiang,Feng Keqiang,Zhao Xueping,Wu Changxing,Zhang Renjun
    1999, 13(2): 104-108 . 
    Abstract ( )   PDF (179KB) ( )  
    Emergence trend and number fluctuation of weeds were observed in fields of wet direct seeded rice in Jiaxing, Zhejiang in 1996-1997. Echinochloa crusgalli, Leptochloa chinensis, Cyperus difformis, Rotala indica, Lindernia procumbens are five main species of weeds, and Mazus japonicus, Ludwigia prostrata, Oenanthe atolonifera and some other weeds are also found in the fields. Numbers of weed species are 25.0%, 18.8%, 56.2% and total numbers of seedling emergence 11.2%, 11.6%, 77.2% for grasses, sedges and broadleaf weeds, respectively. E. crusgalli and L. chinensis are more common in one season rice fields than in other fields, and there are more M. japonicus, L. procumbens, O. atolonifera in early rice fields than in late rice fields. Compared with transplanted rice fields, E. crusgalli, and L. chinensis are more, Eleocharis plantagineiformis, Monochoria vaginalis, O. atolonifera and Ceratophyllum demersum less in the fields of direct seeded rice. Weeds start to emerge in the fields 3 to 4 days after rice is seeded, and emergence peaks appear in 1-3 weeks. For late direct seeded rice fields, the second peak of weed emergence will appear in 6-8 weeks after rice seeded. In the fields of direct seeded rice with tilling cultivation where broadleaf weeds compose the majority of weeds, number of weeds is larger than in non tilling field. The number fluctuation of weeds in direct seeded rice fields showed the same trend as emergence of weed seedlings. In direct seeded rice fields, E. crusgalli, L. chinensis, L. prostrata emerge earliest, followed by R. indica, L. procumbens, M. japonicus and then by C. difformis, O. atolonifera. For E. crusgalli, L. chinensis and other weeds only one peak appears, while for R. indica and L. procumbens the second peak is obvious in the fields of late direct seeded rice.
    Classifying the Ovarian Development of Rice Water Weevil and Its Application
    Zhai Baoping,Shang Hanwu,Cheng Jia’an
    1999, 13(2): 109-113 . 
    Abstract ( )   PDF (391KB) ( )  
    Grading scale for the ovarian development of rice water weevil (RWW), Lissorhoptrus oryzophilus Kuschel, was presented in the light of the practices for pest forecast in China and the operationability in grass roots extension station. According to the morphological changes in ovarian development, their anatomical criteria were classified into six grades, i.e. 0, no oocyte; Ⅰ, previtellogenic; Ⅱ, gravid (vitellogenic to chorionated eggs); Ⅲ, ovipositing (a number of chorionated eggs are stored in the egg calyxes); Ⅳ, late egg laying (the early and middle degeneration); Ⅴ, postoviposition senescence (the late degeneration). These criteria were applied to characterize the field population of RWW and to appraise the suppression effect of some insecticides (say Imidacloprid) that cause the ovarian development retardation and egg laying dysfunction.
    研究简报
    Resistance of Cecropin B Gene Transformed Rice ( Oryza sativa ) and Progenies to Bacterial Blight ( Xanthomonas oryzae pv. oryzae )
    Hua Zhihua,Wang Xiaoling,Xue Rui,Gao Zhenyu,Huang Danian
    1999, 13(2): 114-116 . 
    Abstract ( )   PDF (115KB) ( )  
    The resistance to bacterial blight in different transgenic plantlines of rice variety Jingyin 119 from 6 generations of T 0 to T 5 was primarily studied. Results showed that the resistance to bacterial blight delivered from cecropin B transgene could be segregated and transmitted to high generations. Two resistance improved plantlines were obtained in T 4 generation. From the dynamic change of bacterial infection in different resistance plantlines, it was inferred that cecropin B transgene expression could inhibit the bacterial infection partially, but high level expression of anti bacterial subject was necessary for improving transgenic rice bacterial disease resistance thoroughly.
    Effects of Submergence Treatments on the Growth and Development of Brown Planthopper on Rice Plants and the Nutrient Component in Rice Plants
    Zheng Xusong,Yu Xiaoping,Lu Zhongxian,Chen Jianming,Hu Jicheng
    1999, 13(2): 117-119 . 
    Abstract ( )   PDF (122KB) ( )  
    Breeding and Utilization of Wide Compatibility Rice Restorer Line Chenghui 448
    Ren Guangjun,Lu Xianjun,Li Qingtian,Zhang Chi
    1999, 13(2): 120-122 . 
    Abstract ( )   PDF (116KB) ( )  
    A cross between American rice Lemont and Chinese restorer Line 871028 was conducted in 1989. By using traditional breeding methods and anther culture technology, wide compatibility restorer line Chenghui 448 was bred from the segregation progenies. This line was crossed with indica and japonica test varieties, mean seed setting rates of F1 hybrids are 85.89%,69.04% respectively; with male sterile lines originated from five kinds of different cytoplasm, the average seed setting rate of F1 hybrids is 83.39%. Some superior combinations have been screened by testcross such as Shanyou 448 and Ⅱ you 448, they have passed the regional trial of Sichuan Province in 1997, their mean grain yields in two years are 9.07%,14.51% respectively higher than checks.
    综述与专论
    Progress on Rice Molecular Biology of Resistance to Blast Fungus
    Wu Jianli,Zhuang Jieyun,Li Debao,Zheng Kangle
    1999, 13(2): 123-128 . 
    Abstract ( )   PDF (144KB) ( )  
    Progress on rice molecular biology of resistance to blast fungus was reviewed, which includes molecular markers used for mapping, chromosome location of rice blast resistance genes, allelism of rice blast genes, cloning of avirulence genes from rice blast fungus and the most recent advances towards the cloning of rice blast resistance genes.