Archive

10 July 2007, Volume 21 Issue 4 Previous Issue    Next Issue
---
研究报告
研究简报


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

研究报告

Select

Cytological Analyses and Defense Genes Expression of Disease Resistance to Blast Fungus in Rice WANG Zhong-hua ,LIN Hui ,Barbara VALENT ,J. Neil RUTGER ,JIA Yu-lin 2007, 21(4): 335-340 .  Abstract ( )   PDF (1027KB) ( )   Phenotypical,cytological and molecular responses of rice to the fungus Magnaporthe grisea within 24 h after inoculation were studied by using the lesion mimic mutant LmmKaty and its parent Katy,double haploid rice lines YT14 and YT16 as materials.The variety Katy is susceptible to several virulent M.grisea isolates,and a Sekiguchi like-lesion mimic mutant of Katy(LmmKaty)showed enhanced resistance to these isolates.Autofluorescence was visible under ultraviolet light 20-24 h after localized inoculation in the incompatible interaction,whereas,autofluorescence was not evident in the compatible interaction.The results indicated that rapid cell death was involved in a mechanism of LmmKaty to restrict pathogen invasion.Northern blotting results showed that the rapid accumulation of defense related gene transcripts,phenylalanine ammonia lyase and β-glucanase,was observed beginning at 6 h after inoculation and was obvious at 16-24 h after inoculation in the incompatible interaction.Rapid transcript accumulation of PR-1 and chitinase had occurred 24 h after inoculation in the incompatible interaction.Accumulation of these transcripts was delayed in the compatible interaction.These results indicated that host active defense responses occured 24 h after pathogen inoculation and LmmKaty exhibited enhanced resistance to M.grisea.

Select

Study on Sexual Gland Toxicity of Transgenic Rice with mtlD/gutD Gene in Rats CHEN He ,WANG Hui-zhong ,ZHAO Wen-hua ,CAI Ling-fei ,WANG Ling-jun ,CHEN Xiao-nan ,HUANG Da-nian 2007, 21(4): 341-344 .  Abstract ( )   PDF (27KB) ( )   Sexual gland toxicity of transgenic rice with mtlD/gutD gene(rice line T18-7-8-1)in rats was studied.The results showed that no pathological changes were found in organs such as testis and ovary in rats in both experimental and control groups in a 90-day-feeding test by histological section examination.No significant differences(P>0.05)were observed on maturation of sexual gland in females,aberrant sperm rate of males and sexual hormone of rats between the experimental groups and the control.

Select

Construction of AFLP Fingerprinting and Comparative Analysis Between Three Lines of Cytoplasmic Non-Pollen Type in Yunnan Purple Rice and Their Hybrid F1 HU Kan ,YU Xia-xia ,YU Jin-hong ,DING Yi 2007, 21(4): 345-349 .  Abstract ( )   PDF (343KB) ( )   An AFLP fingerprinting was constructed to conduct comparative analysis on three lines of cytoplasmic non-pollen type in Yunnan purple rice and their hybrid F1.Eight of fourteen random primer pairs displayed significant polymorphisms.In Tianxiang 018(Tianxiang A×Luohui 018)group,including cytoplasmic male sterile line,maintainer line,restorer line and F1,179 bands were amplified,52 of which were polymorphic(29.05%);in Tianxiang 806(Tianxiang A×Luohui 806)group(cytoplasmic male sterile line,maintainer line,restorer line and F1),177 bands were amplified,50 of which were polymorphic(28.25%).Particularly,two specific bands amplified by the primer pair E-ATG/M-CAC were found only in the maintainer line and could obviously distinguish maintainer line from the cytoplasmic male sterile(CMS)line,restorer line and F1 respectively.Some characteristic bands between CMS line and its maintainer line were also observed in other primer pairs,and these would be valuable for identification and utilization of cytoplasmic non-pollen CMS in Yunnan purple rice as well as for further study on mechanism of CMS in hybrid rice.

Select

Predicting Heterosis of Effective Panicle Number per Plant Based on QTL Mapping in Rice ZHAO Yan-hong ,ZHU Jun ,XU Hai-ming ,YANG Jian ,GAO Yong-ming ,SONG You-sheng ,SHI Chun-hai ,XING Yong-zhong 2007, 21(4): 350-354 .  Abstract ( )   PDF (42KB) ( )   An immortalized F2 population in rice derived from F1 hybrid between Zhenshan 97B and Minghui 63,was used to QTL mapping on effective panicle number per plant by using QTLNetwork-2.0.A total of eight QTLs were detected on 6 chromosomes.Both general heterosis and environmental interaction heterosis of effective panicle number per plant in two different environments for F1,F2 and F3 generations were predicted based on QTL effects.It was revealed that QTLs with epistatic effects were prominent contributors to the heterosis for effective panicle number per plant,suggesting that the method of heterosis prediction based on QTL mapping was a powerful tool for screening superior hybrids by means of molecular marker assisted selection.

Select

Genetic Analysis and Molecular Mapping of a Novel Chlorophyll-Deficit Mutant Gene in Rice HUANG Xiao-qun ,WANG Ping-rong ,ZHAO Hai-xin ,DENG Xiao-jian 2007, 21(4): 355-359 .  Abstract ( )   PDF (466KB) ( )   A rice etiolation mutant 824ys featured with chlorophyll deficiency was identified from a normal green rice variety 824B.It showed whole green-yellow plant from the seedling stage,reduced number of tillers and longer growth duration.The contents of chlorophyll,chlorophyll a,chlorophyll b and net photosynthetic rate of the mutant obviously decreased,as well as the number of spikelets per panicle,seed setting rate and 1000-grain weight compared with its wild-type parent.Genetic analysis of F1 and F2 generations of 824ys crossed with three normal green varieties showed that the chlorophyll-deficit mutant character was controlled by one pair of recessive nuclear genes.Genetic mapping of the mutant gene was conducted by using microsatellite markers and F2 mapping population of 495R/824ys,and the mutant gene of 824ys was mapped on the short arm of rice chromosome 3.The genetic distances from the target gene to the markers RM218,RM282 and RM6959 were 25.6,5.2 and 21.8 cM,respectively.It was considered to be a new chlorophyll-deficit mutant gene and tentatively named as chl11(t).

Select

Response of Plasma Membrane H+-ATPase of Rice Root to Ammonium and Nitrate Nutrition DI Ting-jun ,ZHU Yi-yong ,QIU Mei-hua ,KAN Jian-luan ,ZHANG Xiao-man ,XU Guo-hua ,SHEN Qi-rong 2007, 21(4): 360-366 .  Abstract ( )   PDF (1685KB) ( )   Rice plants(Oryza sativa L.ssp.japonica cv.Nanguang)were supplied with ammonium(NH4+-N)or nitrate(NO3--N)as sole nitrogen in hydroponics experiment and the plasma membrane vesicles of rice roots were isolated by a two-phase system at the seedling stage.The plasma membrane H+-ATPase hydrolytic activity was analyzed for elucidating the response of the plasma membrane H+-ATPase of rice root to ammonium or nitrate nutrition.The purity of plasma membrane isolated by the two-phase system was above 95%,which was suitable for analysis of H+-ATPase activity in plasma membrane. The hydrolytic activity, Km and Vmax of H+-ATPase in plasma membrane of rice root supplied with NH4+-N were markedly higher than those with NO3--N.The pH optimum of the plasma membrane H+-ATPase of rice root supplied with NH4+-N was 6.0,while it was about 6.2 for that with NO3--N. In addition,the Western blot showed that the enzyme concentration of plasma membrane H+-ATPase of rice root supplied with NH4+-N was higher than that with NO3--N.The results indicated that the higher activity of H+-ATPase in rice supplied with NH4+-N resulted from the increased amount of H+-ATPase units per membrane area.Furthermore,different H+-ATPase isoforms may exist in the roots of rice supplied with NH4+-N in comparison with NO3--N.It was suggested that the higher activity of plasma membrane H+-ATPase of rice root may be an adaptation mechanism to the NH4+-N nutrition.

Select

Effects of External Calcium on Activities of Antioxidant Enzymes and Membrane Lipid Peroxidation in Rice Seedlings under Nickel Stress HU Ze-you ,DENG Xiao-bo ,PENG Xi-xu ,HE Yan ,LIU Wen-hai ,DAI Guang-yu ,WANG Hai-hua 2007, 21(4): 367-371 .  Abstract ( )   PDF (136KB) ( )   In order to explore the possible physiological mechanisms of calcium involved in improving adaptation to nickel stress in plants,the effects of external calcium(Ca2+)on the activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD)and ascorbate peroxidase(APX),the contents of H2O2 and malondialdehyde(MDA),and electrolyte leakage of leaf tissues were investigated in nickel-stressed rice seedlings.Under 0.1 mmol/L nickel(Ni2+)treatment,the activities of SOD,CAT and APX in rice leaf tissues were significantly decreased,while the activity of POD was obviously enhanced.Moreover,nickel treatments significantly increased the contents of H2O2 and MDA in rice leaf tissues,suggesting oxidative damage and membrane peroxidation occurred in nickel-treated leaf tissues.External calcium promoted the activities of SOD,CAT,APX and POD,but reduced considerably H2O2 and MDA contents and permeability of cell membrane in leaf tissues during the stress period.In addition,the activities of CAT,SOD,POD and APX in 10 mmol/L Ca2+-supplemented leaf tissues were higher,and H2O2 and MDA contents were lesser than those in 5 mmol/L Ca2+ under the nickel stress.It was suggested that one of the mechanisms that external calcium enhanced the tolerance to nickel toxicity in rice seedlings was mediated by improving scavenging of active oxygen species and maintaining the integrity of cell membrane under nickel stress.

Select

Morphological and Ontogenic Characterization of Rice with Dynamic Tiller Angle KANG Wen-qi ,OUYANG You-nan ,ZHANG Shan-qing ,DONG Cheng-qiong ,ZHU Lian-feng ,YU Sheng-miao ,XU De-hai ,JIN Qian-yu 2007, 21(4): 372-378 .  Abstract ( )   PDF (264KB) ( )   The tiller angle is very important for plant architecture and canopy structure in rice.Changes in tiller angle and relevant physiological and ecological characteristics were studied by using three varieties differed in tiller angle(9308,erect;M09,semi-scattered;and DI508,semi-scattered at the tillering stage and erect at the full heading stage)as materials.DI508 is characterized by fast growth at the tillering stage and erect culms after booting and its leaves maintain stable photosynthesis.At the initial tillering stage the tiller angles(between the horizontal and tiller)of the 1st,2nd and 3rd tillers on the main culm in DI508 were 59.74°,62.94° and 68.54°,respectively,similar to M09.After booting the angles changed into 90°,similar to erect rice 9308.The effective tiller rate of DI508 was 68.35%,3.09 percent point higher than that of M09.And the mean dry biomass per plant of DI508 was 155.54 g at the maturity stage,being 15.84% and 64.09% higher than those of M09 and 9308 respectively.Meanwhile,the mean dry biomass per tiller of DI508 was 9.15 g,being 63.69% and 44.78% higher than those of M09 and 9308,respectively.From booting to maturity the photosynthetic ability of the top three leaves of DI508 was stable and strong,with mean net photosynthetic rates of 26.89,25.69 and 24.83 μmol/(m2·s).Moreover,the potential roles of dynamic tiller angle character in rice breeding for high photosynthetic efficiency and high-yielding was discussed.

Select

Preliminary Study on Photochemical Activities in Chloroplasts of Flag Leaf Sheathes for Super Hybrid Rice Liangyoupeijiu GUO Zhao-wu ,XIAO Lang-tao 2007, 21(4): 379-385 .  Abstract ( )   PDF (227KB) ( )   By using a super high-yielding hybrid rice combination Liangyoupeijiu,its male parent 9311,female parent Pei’ai 64S and a three-line hybrid rice combination Shanyou 63 as experimental materials,the techniques of an artificial reaction system for photosynthetic membrane,and 32Pi isotopic labeling and so on,were used to study the photochemical activities of the flag leaf sheathes for Liangyoupeijiu.The means of PS Ⅰ reducing capacity of the flag leaf sheathes and blades for Liangyoupeijiu were 6.35 and 15.31 μmol/(mg·h)higher than those for Shanyou 63,respectively;the means of PSⅡoxygen release activities of flag leaf sheathes and blades for Liangyoupeijiu were 6.16 and 9.89 μmol/(mg·h)higher than those for shanyou 63 respectively,the calcium contents in extracts,the Mg2+-ATPase activities on the chloroplast membranes,the cyclic photophosphorylation activities of the flag leaf sheathes and blades for Liangyoupeijiu were also higher than those for Shanyou 63;the means of noncyclic photophosphorylation activities of the flag leaf sheathes and blades for Liangyoupeijiu were 0.25 and 0.31 μmol/(mg·h)higher than those for Shanyou 63,respectively;the photochemical avtivities of the flag leaf sheathes and blades for Liangyoupeijiu were significantly higher than those of its parents at most stages,showing heterosis over mid-parent or heterobeltiosis.It suggested that rice flag leaf sheath had completely the same kind of the photochemical activity characters as rice flag leaf blade with quantitative differences.High photochemical activities of the flag leaf sheath for Liangyoupeijiu may be one of the important reasons that Liangyoupeijiu performed super high-yielding property.

Select

Relationship Between Diurnal Changes of Net Photosynthetic Rate and Affecting Factors in Flag Leaves of Rice under Saline Sodic Stress YANG Fu ,LIANG Zheng-wei ,WANG Zhi-chun ,CHEN Yuan 2007, 21(4): 386-390 .  Abstract ( )   PDF (125KB) ( )   Relationship between diurnal changes of net photosynthetic rate and affecting factors in flag leaves of rice under saline sodic stress were studied at the full heading stage of rice.The net photosynthetic rate showed a double-peak curve in a day under non-saline sodic and saline sodic stresses.The first peak of net photosynthetic rate appeared at 9:00-10:00 a.m.and 9:00 a.m.,respectively and the second peak of net photosynthetic rate both appeared at 14:00 p.m.The midday depression of net photosynthetic rate always appeared regardless of non-saline sodic or saline sodic stress,but net photosynthetic rate at each time decreased significantly in a day under saline sodic stress.There were some differences in correlation character or correlation degree between net photosynthetic rate and all affecting factors at 9:00-13:00.Under non-saline sodic stress,diurnal changes of net photosynthetic rate were mainly caused by stomatal conductance and limiting stomatal value and stomatal factors served as determinants for diurnal changes of net photosynthetic rate;whereas under saline sodic stress,diurnal changes of net photosynthetic rate were caused by non-stomatal conductance(light intensity,air temperature)and non-stomatal factors were main for diurnal changes of net photosynthetic rate.

Select

Differences of Grain Amylose Contents at Different Positions within a Panicle Between the Compact-and Loose-Panicle Rice Varieties LU Yan-ting ,ZHANG Xiao-ming ,YE Sheng-hai ,QI Yong-bin ,JIN Qing-sheng 2007, 21(4): 391-395 .  Abstract ( )   PDF (552KB) ( )   The variation of amylose content(AC)among grains at different positions within a rice panicle was investigated with compact and loose panicle types of japonica rice varieties Zhejing 22 and Zhejing 27 as materials.The differences between the maximum and minimum of grain AC in Zhejing 22 and Zhejing 27 reached 13.53 and 10.30 percent point,respectively.The variation range of AC in compact panicle is larger than that in loose panicle.The AC for each grain in a panicle followed a normal distribution.The correlation coefficients between grain AC and its position in the primary and secondary branches of the panicle in compact and loose panicle types were-0.1167 and-0.3140,respectively,showing that the coefficient of relationship in the loose panicle type was more significant than that in the compact type,and in addition,the coefficient of relationship between AC and grain position in the primary branch was higher than that in the secondary one.The AC of the grains from the top of branch was higher than that of the second grain under the first top one.The distribution of grain AC in a panicle was similar between the loose panicle type and the compact panicle type.It was better to choose the third or forth grains in the panicle branch for AC analysis if whole or half a grain sample should be determined.

Select

Response of Seed-Setting and Grain Quality of Rice to Temperature at Different Time During Grain Filling Period SHENG Jing ,TAO Hong-juan ,CHEN Liu-gen 2007, 21(4): 396-402 .  Abstract ( )   PDF (1125KB) ( )   An experiment was conducted with two rice varieties Huajing 1 and Teyou 559 in plant growth chamber with four temperature treatments for each of six times during grain filling period to investigate the response of seed-setting and grain quality to temperature at different time during grain filling.Seed setting rate and grain weight descended significantly,and appearance and eating qualities were worse after exposure to high temperature(40℃)at the filling stage.Under the experimental conditions,the optimum diurnal air temperatures for high seed setting rate,grain weight and good qualities were 35℃ and 30℃ at the filling stage for Huajing 1 and Teyou 559,respectively.Moreover,the response of seed-setting and grain quality to temperature differed with the temperature treated time.The most sensitive time of seed setting rate and grain weight to high temperature was at 1-5th and 11-20th day after flowering.However,high temperature at 16-20th day after flowering produced the most inferior appearance and eating qualities.High temperature had slight effect on grain quality at the later grain-filling stage(25 days after flowering).Therefore,temperature conditions during grain filling is one of the most important agricultural factors affecting rice good quality production.

Select

Effect of Controlled Release Nitrogen Fertilizer on Protein Quality of Brown Rice and Key Enzyme Activity Involved in Nitrogen Metabolism in Hybrid Rice LUO Lan-fang ,ZHENG Sheng-xian ,LIAO Yu-lin ,NIE Jun ,XIE Jian ,XIANG Yan-wen 2007, 21(4): 403-410 .  Abstract ( )   PDF (159KB) ( )   The protein and amino acids contents and components in brown rice,the activities of nitrate reductase(NRase),glutamine synthetase(GS)and protease in functional leaves and grains in hybrid rice as regulated by controlled release nitrogen fertilizer(CRNF)were analyzed by using Baliangyou 100(early hybrid rice)and Weiyou 46(late hybrid rice)as materials.There were three treatments:1)Control(no nitrogen applied,CK);2)Urea(90 kg N/hm2 in early hybrid rice,105 kg N/hm2 in late hybrid rice,2/3 N as basal application,1/3 N as top-dressing at the tillering stage,Urea);3)Controlled release nitrogen fertilizer(90 kg N/hm2 in early hybrid rice,105 kg N/hm2 in late hybrid rice,all N as basal application,CRNF).Results showed that total protein and complex protein contents in brown rice of both the early and late hybrid rice were significantly higher in CRNF treatment than in urea treatment.Glutelin and glutelin/prolamine ratio were increased significantly under CRNF treatment for both of the hybrid rice combinations.Total amino acids,essential amino acids and limited amino acids contents in brown rice under CRNF treatment for both of the hybrid rice combinations were increased greatly compared with urea treatment.Dynamic changes in NRase and protease activities in functional leaves and glutamine synthetase activities in grains for both of the hybrid rice combinations followed the same tendency.Moreover,CRNF application was favorable to maintain the activities of NRase,GS and protease at high level.CRNF application had obvious regulation for key enzyme activities involved in nitrogen metabolism in functional leaves and grains of hybrid rice,so it might contribute to a significantly enhanced grain yield,protein content and amino acids content of hybrid rice comparing with urea application.

Select

Effects of Different Nitrogen Fertilizer Levels on Concentrations of Fe,Mn,Cu and Zn and Grain Quality in Rice (Oryza sativa) HAO Hu-lin ,WEI You-zhang ,YANG Xiao-e ,FENG Ying ,WU Chun-yong 2007, 21(4): 411-416 .  Abstract ( )   PDF (146KB) ( )   In a pot experiment at greenhouse,effects of N fertilizer on concentrations of Fe,Mn,Cu and Zn in shoot of rice and quality of brown rice were studied.With the increase of N fertilizer application,the concentrations of Fe,Mn,Cu and Zn in shoot increased.Effects of N fertilizer on IR68144 were similar to IR64,but different in the contents of trace elements in plant,suggesting that characteristic expression of the tested two genotypes is not controlled by the amount of N fertilizer supplied.Concentrations of those microelements in brown rice increased at first and then decreased with the increase of N fertilizer application.Contents of Fe,Mn,Cu and Zn in brown rice of the two varieties reached the highest level at 160 kg/hm2 N.Comparing with control,contents of Fe,Mn,Cu and Zn in brown rice of IR64 increased by 28.96%,41.34%,58.31% and 16.0%,while those of IR68144 increased by 22.16%,13.75%,8.75% and 20.21%,respectively.Meanwhile,N fertilizer promoted the accumulation of protein in grains,decreased the accumulation of amylose,and enhanced gel consistency of brown rice.Those results indicated that appropriate N fertilizer management could increase micronutrient content in grains and improve nutrition quality of rice.

Select

Relationship Between Weed Seeds Dispersed by Irrigation Water and Soil Weed Seedbank of Paddy Field in Rice-Growing Region ZUO Ran-ling ,QIANG Sheng ,LI Ru-hai 2007, 21(4): 417-424 .  Abstract ( )   PDF (125KB) ( )   In order to study the correlation between soil weed seedbank and weed seed dispersed by irrigation water,three plots of paddy fields with an irrigation system in a typical rice-growing region was chosen to observe weed community and seedbank both in surrounding habitats and paddy field.The species and number of weed seeds that dispersed by irrigation water were recorded and counted,the weed communities on the ridge of the paddy field and in surrounding habitats of the ditch were surveyed,and they were compared with those in wheat field of next season.The weed seeds of 21 species belonging to 14 families(mainly Gramineae,Primulaceae,Polygonaceae,and Chenopodiaceae)could be dispersed into the paddy field by irrigation water.Seeds of 41 weed species belonging to 19 families were found in the soil weed seedbank of paddy field.The similarity among weed species dispersed by irrigation water,weed communities on the ridge of the paddy field and in the surrounding habitats of the ditch,successive weed communities in wheat field and soil weed seedbank in paddy field was considerablely high with Sorensen similarity indexes all above 0.5,suggesting that weeds were dispersed among them by irrigation water.

Select

Evaluation of Rice Germplasm for Resistance to the Small Brown Planthopper and Analysis on Resistance Mechanism DUAN Can-xing ,ZHANG Shi-xian ,CHEN Qing ,CHENG Zhi-jun ,ZHAI Hu-qu ,WAN Jian-min 2007, 21(4): 425-430 .  Abstract ( )   PDF (135KB) ( )   One hundred and thirty-eight rice accessions were evaluated for resistance to the small brown planthopper(SBPH)(Laodelphax striatellus Fallén)by seedbox screening test with modification.Twenty-five rice accessions with different levels of resistance to SBPH were detected,accounting for 18.1% of the total accessions,which included two highly resistant,nine resistant and 14 moderately resistant varieties.Compared with indica rice,japonica rice was more susceptible to SBPH.Antixenosis test,antibiosis test and correlation analysis were performed to elucidate the resistance mechanism.The highly resistant varieties such as Rathu Heenati(RHT),Mudgo and Kasalath and resistant variety IR36 expressed strong antixenosis and antibiosis against SBPH,indicating the close relationship between resistance level and these two resistance mechanisms in the four rice varieties.Antibiosis was the dominant resistance pattern in the resistant varieties Daorenqiao and Yangmaogu due to their high antibiosis.Dular,ASD7 and Milyang 23 had relatively strong antixenosis and antibiosis,indicating the two resistance mechanisms were significant in these three varieties.The resistant variety DV85 expressed relatively high level of antixenosis but low antibiosis,while Zhaiyeqing 8 and Guiyigu conferred only moderate antibiosis and antixenosis to SBPH,suggesting tolerance in these three varieties.Antibiosis and antixenosis governed the resistance to SBPH in the moderately resistant variety 9311.Antixenosis was the main resistance type in V20A.Tolerance was considered to be an important resistance mechanism in Minghui 63 and Yangjing 9538 due to their poor antibiosis and antixenosis resistance.The above accessions with strong antibiosis or antixenosis were the ideal materials for resistance breeding.

Select

Comparison of Burkholderia cepacia Complex Genomovars from Rice Rhizosphere and Clinic ZHANG Li-xin ,SONG You-liang ,LUO Yuan-chan ,LI Bin ,XIE Guan-lin 2007, 21(4): 431-435 .  Abstract ( )   PDF (491KB) ( )   Burkholderia cepacia is regarded as genetically distinct but phenotypically similar bacterial group referring to Burkholderia cepacia complex(Bcc).It’s not only found in clinic but also in rice growing environment.It is very important in microbial safety of rice to understand the genomovar status of Bcc.Genomovar analysis was performed among 87 Bcc isolates by means of Hae Ⅲ-recA RFLP assays and species-specific PCR tests.Three genomovars were found in the rice rhizosphere includingⅠ,ⅢB and Ⅴ,and genomovarⅤwas the predominant.GenomovarsⅠ,ⅢA and ⅢB existed in the clinical samples,and genomovar ⅢA was the most popular.It showed genomovar composition was different between the Bcc strains from the rice rhizosphere and clinical environment.Moreover,the results revealed the genetic diversity of Bcc strains from the rice rhizosphere,and genomovar Ⅲ referred as virulent species in clinic also existed in the rice rhizosphere.

研究简报

Select

QTL Mapping for Leaf Malondialdehyde Content Associated with Stress Tolerance in Rice JIANG Jing ,ZHUANG Jie-yun ,FAN Ye-yang ,SHEN Bo 2007, 21(4): 436-438 .  Abstract ( )   PDF (220KB) ( )   Malondialdehyde(MDA)is the final product of lipid peroxidation,and its content can reflect stress tolerance of plants.By employing a recombination inbreed line(RIL)population involving 247 lines derived from an indica-indica cross Zhenshan 97B× Milyang 46,a linkage map consisting of 207 DNA markers was used for genetic analysis and gene mapping of malondialdehyde(MDA)content in rice leaves.The MDA content in the RIL population showed transgressive segregation.Two QTLs for MDA content of rice leaves were detected at intervals RG532-RG811 and RG381-RG236 on chromosome 1,accounting for 4.33% and 4.62% of phenotypic variation.Their additive effects were from both of parents.

Select

Identification of a Herbicide Safener AD-67 Inducible cDNA in Rice YIN De-suo ,SUN Xiao-qiong ,LI Ke ,WANG Shi-quan ,DENG Qi-ming ,LI Ping 2007, 21(4): 439-442 .  Abstract ( )   PDF (134KB) ( )   A safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display.The transcript increased 6 h after the rice seedlings were sprayed with the safener solution and 5 days later,the expression could still be detected.The fragment was recycled from the poly-gel and sequenced,and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice data base,and showed 60%-84% homologous to the gene Yippee in several eukaryotes in amino acid sequence.The fragment was extended to a 831 bp cDNA,a primer was designed and RT-PCR analysis supported the induction result.

Select

SSLP-Based SSR Fingerprinting and indica/japonica Classification of Yongyou Series Hybrid Rice LU Yong-fa ,MA Rong-rong ,WANG Xiao-yan ,LI Xin-nian ,ZHOU Hua-cheng ,ZHANG Zhi-yuan ,HUA Guo-lai 2007, 21(4): 443-446 .  Abstract ( )   PDF (69KB) ( )   Three standard indica test varieties,three standard japonica test varieties and other thirteen rice accessions,which are Yongyou series hybrid rice combinations and their parents,were analyzed by using 22 simple sequence repeat(SSR)primer pairs.Totally 87 alleles were detected.These primers revealed polymorphism with a range of 2-8 alleles per primer pair.Fingerprintings of Yongyou series hybrid rice combinations and their parents were set up.The cluster analysis showed that the tested materials were grouped into two clusters in accordance with the indica/japonica classification based on the Cheng’s index.It was confirmed that Yongyou 4 and Yongyou 6 are inter-subspecific hybrid rice combinations between indica and japonica at molecular levels.