Chinese Journal OF Rice Science

Chinese Journal of Rice Science

• 研究简报 • Previous Articles     Next Articles

RAPD Analysis of A New Resistance Gene Xa-25(t) to Rice Bacterial Blight

GAO Dong-ying 1; 2; XIANG Yang-hai 2; SUN Li-hua 2; LU Zuo-mei 1   

  1. (1Department of Agronomy; Nanjing Agricultural University; Nanjing 210095; China; 2Institute of Agrobiological Genetics and Physiology; Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-04-10 Published:2002-04-10

抗水稻白叶枯病新基因Xa-25(t)的RAPD分析

高东迎 1，2; 向阳海 2; 孙立华 2; 陆作楣 1   

  1. 1南京农业大学 农学系， 江苏 南京 210095； 2 江苏省农业科学院 农业生物遗传生理研究所， 江苏 南京 210014

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Abstract: Xa-25(t), a new resistance gene to bacterial blight identified from the somaclonal mutant HX-3, was studied by using RAPD marker. Out of the 306 random primers tested,two were found to be polymorphic between the parents and the resistant pool and susceptible pool(which were composed of 12 highly resistant plants and highly susceptible plants,respectively). With the two primers, 114 individuals from the F2 population of Longtefu A/HX-3 were analyzed for linkage relationship. Results indicated that S1269 and S1327 were located on the opposite sides of Xa-25(t), with the distances of 5.3 cM and 23.7 cM respectively.

Key words: rice, bacterial blight, somaclonal mutant, disease resistance, gene, randomly amplified polymorphic DNA

摘要： 利用RAPD标记对从体细胞突变体HX-3中鉴定出的抗水稻白叶枯病新基因Xa-25 (t)进行了研究，在306个随机引物中，两个引物S1269和S1327在亲本和抗感池（均由12个高抗或高感植株组成）之间表现多态性。用这两个引物对龙特甫A和HX-3的F2群体的114个单株进行连锁分析，表明 S1269和 S1327与Xa-25 (t)的连锁距离分别为5.3和23.7 cM，且位于Xa-25 (t)的两侧。

关键词: 水稻, 白叶枯病, 体细胞突变体, 抗病性, 基因, 随机扩增多态性DNA