Abstract: Some rice varieties were transformed with glgC-TM gene mediated by Agrobacterium.It was showed that hygromycin had a good selective effect on non transformed calli. During the transformation, it was not recommended to use mature embryos as explants and take native carbenicillin as antibiotics. Varieties and plasmids had little effects on the formation of hygromycin resistant calli and percentage of positive transformed plantlets. Results of PCR analysis in T 1 progenies indicated that a homozygous line could be obtained in T₀ generation.

Key words: rice, Agrobacterium tumefaciens, gene transfer, adenosine diphosphate glucose pyrophosphatase gene

摘要： 利用农杆菌介导将glgC-TM基因导入多个水稻品种。研究表明农杆菌转化过程中，潮霉素是一种很好的筛选剂，成熟胚以及国产的羧苄青霉素不适宜用作农杆菌介导的遗传转化。不同品种以及不同的质粒在转化过程中对抗性愈伤的得率和阳性植株的转化率不存在显著影响。T1代PCR检测表明，在转基因当代就有可能获得转基因纯系。

关键词: 水稻, 农杆菌, 基因转化, 葡萄糖腺苷二磷酸焦磷酸化酶基因