Abstract: F₁ of indica type dual purpose genic male sterile (DGMS) rice was used to anther culture. Some DH lines with pale green leaf marker developed from anther derived lines obtained from M2S/Pei’ai 64S cross were selected into growth chambers to study the sterile expression and fertile transformation of their responses to photoperiod and temperature. The difference of their responses to photoperiod and temperature was large among DH lines. Results included F 2 and BC 1 segregation indicated that when DGMS gene(s) was transferred from Nongken 58S of a japonica subspecies with sensitive to light to M2S of a indica DGMS line with weakly sensitive to light, DGMS line was controlled by two main recessive genes and modified clearly by minor genes. The character of pale green leaf was controlled by one gene. No linkage existed between DGMS genes and pgl gene. The conditions about researching on inheritance of DGMS genes, the effects of anther culture and actions of DGMS development are discussed.

Key words: dual purpose genic male sterile line, marker gene, anther culture, inheritance, Oryza sativa

摘要： 利用花药培养技术对籼型两用核不育水稻的杂种F1进行花药培养，获得了大量的绿苗，从组合M2S / 培矮S中选育出一批带淡绿叶标记的籼型两用核不育DH品系，选择部分DH系进入人工气候箱鉴定其光温反应特性，结果表明DH系之间的光温反应特性差异较大。通过亲本、F1、F2和BC1世代的遗传研究表明，两用核不育基因从感光的晚粳农垦58S转导至弱感光的籼稻M2S后，表现为由2对主基因控制，微效基因的修饰作用明显；淡绿叶性状由1对基因控制，两用核不育基因与淡绿叶基因之间不存在连锁。还对两用核不育基因的遗传分析条件、花药培养效果及其在两用核不育系选育中的作用进行了讨论。

关键词: 两用核不育系, 标记基因, 花药培养, 遗传, 水稻