Abstract: A detached rice appraisement method for evaluating the resistance of conventional rice and transgenic rice to striped stem borer was developed. This method overcame problems such as: stems were difficult to maintain freshly in longer time, operation was complex, and striped stem borers often escaped and so on in previous methods. It was reliable and the operation could be easily normalized.

Key words: rice, striped stem borer, isolated culture, resistance, screening, methodology

摘要： 提出了一套规范的水稻抗二化螟离体稻株鉴定技术。该项技术克服了以往鉴定方法难保鲜、操作烦琐、试虫易逃逸等问题，准确可靠、操作易规范，适用于常规水稻和转基因水稻抗二化螟特性鉴定和评价。

关键词: 水稻, 二化螟, 离体培养, 抗性, 鉴定, 研究法