Abstract:

In silico cloning was a new way of gene cloning by bioinfomatics, which developed with genome enforcement and EST project. Since the rice draft sequence of genome was published in public, it was possible to isolate the functional genes of rice by in silico cloning. The principle, application, related resource of bioinformatics, problems and the prospect of in silico cloning of functional genes in rice were reviewed.

Key words: rice, in silico cloning, bioinformatics, functional gene

摘要： 电子克隆是随着基因组计划和EST计划实施发展起来的，利用生物信息学手段进行基因克隆的新方法。已经公布的水稻基因组序列框架图，使得水稻的电子克隆路线得以实施。介绍了水稻电子克隆的基本原理、应用实例、相关的生物信息资源、存在问题和展望。

关键词: 水稻, 电子克隆, 生物信息学