Abstract: Zhu 1S is an indica male sterile line with low critical temperature of fertility change, high ability of resistance, and good combining ability. For improving its unexpected plant height, somaclonal lines were established by culturing explants including young spikelets and mature embryos. Variants were obtained from somaclonal lines by subculturing these calli and treating them with chemical reagent. The variation percentage was above 40%. Somaclonal variant lines,SV5,SV10 and SV14, were got from variants above. They were dwarfing variants and could inherit stably. Dwarfing variant SV14 had obvious superiority in combining ability and rice yield, it might be spreaded widely in rice production.

Key words: rice, somaclonal lines, dwarf, mutant, male sterile line

摘要： 以幼穗和成熟胚为外植体经组织培养成功建立了体细胞无性系，结合愈伤组织多代培养和化学物质诱导，获得了高达40%以上的体细胞无性系变异，并从中选育出遗传稳定的株1S矮秆突变体SV5、SV10和SV14。鉴定结果表明矮秆突变体SV14是矮化了的光温敏核不育系，具有优良的光温敏不育特性，配合力强，杂种优势明显，较对照可以增产10%以上，具有较大的推广价值。

关键词: 水稻, 体细胞无性系, 矮秆, 突变体, 雄性不育系