Chinese Journal OF Rice Science

Chinese Journal of Rice Science

• 研究报告 • Previous Articles     Next Articles

Expression and Mapping of cDNA Fragments Responsive to Magnaporthe grisea in Rice

RAO Zhi-ming 1; DONG Hai-tao 2; ZHUANG Jie-yun 1; CHAI Rong-yao 3; FAN Ye-yang 1; LI De-bao 2; *; ZHENG Kang-le 1; *   

  1. （1Chinese National Center for Rice Improvement; China National Rice Research Institute; Hangzhou 310006; China; 2Key Laboratory of Plant Pathology and Biotechnology; Ministry of Agriculture; P. R. China; Zhejiang University; Hangzhou 310029; 3Institute of Plant Protection; Zhejiang Academy of Agricultural Sciences; Hangzhou 310029, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-04-10 Published:2003-04-10

水稻稻瘟病菌胁迫应答cDNA片段的表达及定位

饶志明1; 董海涛2; 庄杰云1; 柴荣耀３; 樊叶杨1; 李德葆2,*; 郑康乐1,*   

  1. 1中国水稻研究所 国家水稻改良中心, 浙江 杭州 310006； 2浙江大学 农业部植物病理学重点实验室， 浙江 杭州 310029； 3浙江省农业科学院 植物保护研究所， 浙江 杭州 310021

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Abstract: The expression profiles of a resistant line G205 derived from a RIL population of Zhong 156×Gumei 2 in response to Magnaporthe grisea were investigated by using cDNA microarray containing 2200 expressed sequence tags (ESTs). One cDNA fragment encoding nucleotide binding site (NBS) and leucine rich repeat (LRR) was up regulated and the other cDNA fragment encoding nitrilase associated protein (NIT) was down regulated significantly by the fungus, which were confirmed by Northern blot. They were mapped on chromosomes 2 and 3 by RFLP analysis respectively. Both of them were located on the intervals where QTLs controlling partial resistance to blast had been detected.

Key words: rice, cDNA microarray, nitrilase, gene mapping, Magnaporthe grisea

摘要： 以抗稻瘟病品系G205为材料，应用cDNA微阵列分别获得了一个受稻瘟病菌诱导的含NBSLRR的cDNA克隆（暂命名为RIM1, rice induced by Magnaporthe grisea）和一个受稻瘟病菌抑制的编码腈水解酶（Nitrilase）的cDNA克隆（暂命名为NIT），并通过Northern得到证实。RFLP分析将RIM1和NIT分别定位于水稻第2和第3染色体上，它们均位于控制水稻稻瘟病部分抗性QTL区间。

关键词: 水稻, cDNA微阵列, 腈水解酶, 基因定位