Chinese Journal of Rice Science

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Analysis of Introgressed Segments in Near-Isogenic Lines for F1 Pollen Sterility in Rice

LI Wen-tao; ZENG Rui-zhen; ZHANG Ze-min; ZHANG Gui-quan *   

  1. (Plant Molecular Breeding Research Center, South China Agricultural University, Guangzhou 510642, China)

  • Received:1900-01-01 Revised:1900-01-01 Online:2003-04-10 Published:2003-04-10

水稻F1花粉不育性近等基因系导入片段的分析

李文涛; 曾瑞珍; 张泽民; 张桂权*   

  1. 华南农业大学 植物分子育种研究中心, 广东 广州 510642

Abstract: One hundred and fifty eight microsatellite markers,with polymorphism among parents were used to survey the introgressed segments in 50 F 1 pollen sterility near isogenic lines. Two hundred and sixty introgressed segments were detected in 50 introgression lines, each carrying 5.2 introgressed segments. Among them,100 introgressed segments carrying F1 pollen sterility loci concentrated on the region of F 1 pollen sterility genes while 160 introgressed segments not related to F1 pollen sterility loci distributed randomly over 12 chromosomes. Both the average number and length of introgressed segments decreased along with the increase of backcross generations. The number of introgressed segments was less than four and the length of introgressed segments was less than 20 cM beyond the third generations (BC3).

Key words: introgressed segment, near isogenic line, pollen sterility, rice

摘要: 选用158个亲本间有多态性的微卫星标记,对50个F[sub]1[/sub]花粉不育近等基因系的导入片段进行了分析。50个近等基因系中共检测出260个导入片段,平均每个近等基因系有5.2个。其中100个F[sub]1[/sub]花粉不育基因所在的导入片段集中分布于第1、第3和第5染色体上,其余160个非F[sub]1[/sub]花粉不育基因所在的导入片段随机分布于各染色体上。统计分析表明,平均导入片段数和平均导入片段长度均随回交世代的增加而逐渐减少。BC[sub]3[/sub]代以后两者趋向稳定,导入片段数均少于4个,导入片段长度均在20 cM以下。

关键词: 导入片段, 近等基因系, 花粉不育性, 水稻