Chinese Journal OF Rice Science

Chinese Journal of Rice Science

• 实验技术 • Previous Articles    

Determination of Amylose Content in Single Rice Grain by Near Infrared Transmittance Spectroscopy(NITS)

XIAO Xin; CHEN Yi; LUO Wen-yong; LIU Yan-zhuo; MAO Xing-xue; LI Xiao-fang *   

  1. （Guangdong Key Laboratory of New Biotechnology in Rice Breeding; Rice Research Institute; Guangdong Academy of Agricultural Sciences; Guangzhou 510640; China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-07-10 Published:2003-07-10

单粒活体稻谷种子直链淀粉含量的近红外透射光谱分析

肖昕; 陈奕; 罗文永; 刘彦卓; 毛兴学; 李晓方*   

  1. 广东省农业科学院 水稻研究所, 广东省水稻育种新技术重点实验室， 广东 广州 510640

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Abstract: Two hundred and twenty-two samples of single rice seed were scanned by FOSS Tecator Infratec 1255 Analyzer with Single Seed Adapter and the amylose contents were determined by reference method. With the powerful WinISI calibration maker software, several mathematics treatments to the sample spectra and several different regression methods were adopted to optimize a good calibration equation for determination of amylose content in a single rice grain. The standard error of calibration (SEC), standard error of cross-validation(SECV), validation performance (SEP) and regression squared (RSQ) of the calibration selected were 2.828, 3.088, 2.792 and 0.848, respectively.

Key words: near infrared transmittance spectroscopy, single seed, amylose content, rice, methodology

摘要： 利用FOSS Tecator公司的Infratec 1255型带单粒定标器的近红外谷物分析仪，对222粒单粒稻谷进行扫描并测定了直链淀粉含量的参比数据。借助于功能强大的近红外定标器软件(WinISI)，采用多种计量数学处理方法和不同的回归统计方法进行定标曲线的开发和比较，优化得到了单粒水稻种子直链淀粉含量测定的近红外定标方程。其定标标准偏差（SEC）、交叉检验标准误差（SECV）、检验工作标准误差（SEP）和定标相关系数（RSQ）分别为2.828、3.088、2.792、0.848。

关键词: 近红外透射光谱, 单粒种子, 直链淀粉含量, 水稻, 研究方法