SSR Analysis and Soluble Protein Content in Leaf of Rice Mutants Induced by High Pressure

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SSR Analysis and Soluble Protein Content in Leaf of Rice Mutants Induced by High Pressure

HE Xiu-ying 1; XU Shi-ping ²; LIAO Yao-ping ¹; MAO Xing-xue ¹; WENG Ke-nan ²; CHEN Zhao-ming ¹; CHEN Yue-han¹; XIAO Wan-sheng ²

(¹Rice Research Institute; Guangdong Academy of Agricultural Sciences; Guangzhou 510640, China; ²Guangzhou Institute of Geochemistry; Chinese Academy of Sciences, China)

Received:1900-01-01 Revised:1900-01-01 Online:2003-10-10 Published:2003-10-10

水稻高压变异材料的SSR标记和叶片可溶性蛋白质含量分析

何秀英1; 徐世平2; 廖耀平1; 毛兴学1; 翁克难2; 陈钊明1; 陈粤汉1; 肖万生2

1广东省农业科学院水稻研究所，广东广州 510640； 2中国科学院广州地球化学研究所，广东广州 510640

Abstract

Abstract: Rice variety Yuexiangzhan and its mutants induced by high pressure, were studied by using microsatellite marker and soluble protein content analysi s. Results indicated that 11 of the 88 microsatellite primer pairs showed polymorphism. The polymorphic frequency was 3.4%-11.3% between mutants and Yuexiangzhan. The polymorphic markers were randomly located on rice chromosomes. The more similar the plant type of the mutants looked like their original variety(Yuexiangzhan), the less polymorphic loci were obtained. In addition, content of soluble protein in leaves of mutant was significantly lower than that in the original variety.

Key words: high pressure, mutant, microsatellite marker, protein content, rice

摘要： 应用微卫星标记技术和可溶性蛋白质含量测定，对水稻品种粤香占及其由高压引起的变异材料粤压1、2、3、4号进行分子生物学研究。结果表明，在选取的88对微卫星引物中有11对表现多态性，变异材料与原种的多态性频率介于3.4%~11.3%，多态性标记随机地分布于水稻染色体上，形态上与原种越相近的材料多态性位点数越少。此外，每一变异材料叶片可溶性蛋白质含量互不相同，均显著低于对照。

关键词: 高压, 变异材料, 微卫星标记, 蛋白质含量, 水稻

HE Xiu-ying ,XU Shi-ping ,LIAO Yao-ping ,MAO Xing-xue ,WENG Ke-nan ,CHEN Zhao-ming ,CHEN Yue-han ,XIAO Wan-sheng . SSR Analysis and Soluble Protein Content in Leaf of Rice Mutants Induced by High Pressure[J]. Chinese Journal of Rice Science.

何秀英, 徐世平, 廖耀平, 毛兴学, 翁克难, 陈钊明, 陈粤汉, 肖万生. 水稻高压变异材料的SSR标记和叶片可溶性蛋白质含量分析[J]. 中国水稻科学.

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