Abstract: Four transgenic rice lines harboring binary chitinase gene and β-1,3-glucanase gene were challenged in the greenhouse by inoculating 33 isolates belonging to 24 races of Magnaporthe grisea collected from Yunnan Province. Results showed that transgenic rice lines exhibited high resistance to 20 isolates which could infect the receptor cultivar Nan 29 and almost all of the isolates which could not infect the receptor cultivar. It indicated that introduction of binary chitinase gene and β-1,3-glucanase gene into restorer line of Dian-type hybrid rice enhanced resistance to blast, and the transgenic rice lines possessed wide-spectrum resistance against various rice blast races. Disease score of leaf blast were 0-1 degree and disease incidence of neck blast were 0.3%-10.23% in the transgenic lines under the blast nursery, showing resistance to the disease enhanced in transgenic line. It suggested that these transgenic rice lines could be available for breeding resistant variety.

Key words: rice, transgene, chitinase gene, β-1, 3-glucanase gene, rice blast, resistance spectrum

摘要： 用来源于云南各地属于24个稻瘟病菌（[i]Magnaporthe grisea[/i]）生理小种的33个菌株，对受体品种南29及其4个转几丁质酶β-1,3-葡聚糖酶串联基因水稻T5代品系进行了温室接种鉴定。结果表明，不能侵染受体品种南29 的13个菌株也不能侵染转基因品系，可侵染受体品种南29 的20个菌株一般不能侵染转基因品系，转基因水稻对稻瘟病菌抗性范围较受体对照扩大，证明转几丁质酶葡聚糖酶基因水稻对稻瘟病具有一定的广谱抗性；诱发条件下，转基因品系大田叶瘟病情指数为0～1级，穗瘟发病率为0.3%～10.23%，抗性较受体对照大幅度提高，表明这些品系在稻瘟病抗病育种中是有应用潜力的。

关键词: 水稻, 转基因, 几丁质酶基因, 葡聚糖酶基因, 稻瘟病