Abstract: The interaction between rice host and its pathogen Xanthomonas oryzae pv. oryzae (Xoo) at cell level was studied using a resistant somaclonal mutant HX-3 and its donor Minghui 63. After inoculation of Xoo strain Zhe 173(Chinese pathotype Ⅳ), the activity of superoxide dismutase(SOD) and peroxidase(POD) in the callus of Minghui 63 was increased dramatically, and the active oxygen was produced at a higher rate; Meanwhile, the callus grew slowly with the reduction of protein content. However, the activity of SOD and POD, the product ion rate of active oxygen and the fresh weight in HX-3 callus varied little after inoculation. It could be proposed that there were great differences between the resistance of HX-3 and Mighui 63 at cell level. There was no difference in resistance to bacterial leaf blight in HX-3 between the plant and the callus.

Key words: rice, bacterial leaf blight, somaclonal mutant, active oxygen, resistance to disease, callus

摘要： 对水稻抗白叶枯病体细胞突变体HX-3及其感病供体明恢63在细胞水平上与白叶枯病菌的互作进行了研究。结果表明，接种水稻白叶枯病菌浙173后，明恢63愈伤组织的超氧化物歧化酶（SOD）和过氧化物酶（POD）活性均急剧大幅升高，O[sub]2[/sub]产生速率升高，而愈伤组织生长量减小，蛋白质含量下降。HX-3愈伤组织接菌后SOD、POD活性和O[sub]2[/sub]产生速率与未接菌愈伤组织相似，愈伤组织生长量减小不明显。说明HX-3与明恢63的细胞水平抗性存在较大差别，HX-3细胞水平抗性与植株水平抗性是一致的。

关键词: 水稻, 白叶枯病, 体细胞突变体, 活性氧, 抗病性, 愈伤组织