Abstract: S-b is one of the hybrid sterility loci between indica and japonica cultivars in rice. The genotype of Taichung 65 (T65) and Guanglu’ai 4 (GLA 4) at the locus is S^j/S^j and Sⁱ/Sⁱ, respectively. Four indica-compati ble japonica lines (ICJLs), G2416-3, G2417-2-1, G2605 and G3004-4 were cros sed with testers, T65 and GLA 4, and their S-b locus genotypes were identified. The segregation of PCR-based molecular markers linked tightly with S-b locus in F₂ population showed that G2416-3 and G2417-2-1 possessed Sⁱ/Sⁱ genotype, while G2605 and G3004-4 contained genotype Sⁿ/Sⁿ. Comparison of genetic effect among the alleles at S-b locus in four ICJLs showed no significant difference between the Sⁱ genes in G241 6-3 and G2417-2-1, and between the Sⁿ genes in G2605 and G3004-4, but significant difference between the Sⁱ and Sⁿ genes.

Key words: rice, indica-compatible japonica lines, pollen ster ility, multi-alleles, microsatellite marker

摘要： S-b是控制水稻籼粳亚种间杂种不育性的基因座位之一。在该座位，台中65的基因型为Sj/Sj，广陆矮4号的基因型为Si/Si。以台中65和广陆矮4号为遗传测验种，分别与4个粳型亲籼系配组，根据紧密连锁分子标记基因型在这些组合F2群体中的偏态分离程度， 测定了这4个粳型亲籼系在F1花粉不育性S-b基因座位的基因型，结果表明，G2416-3和G2417-2-1在S-b基因座位的基因型为Si/Si，而G2605和G3004-4的基因型为Sn/Sn。比较了不同粳型亲籼系S-b基因座位等位基因遗传效应的大小，结果表明G2416-3携带的Si基因与G2417-2-1携带的Si基因之间，以及G2605携带的Sn基因与G30044携带的Sn基因之间没有显著差异，而Si 与Sn之间遗传效应差异极显著。

关键词: 水稻, 粳型亲籼系, 花粉不育性, 复等位基因, 微卫星标记