Determination of Cytokinins in Rice Root Exudates by High Performance Liquid Chromatography(HPLC)

Chinese Journal of Rice Science

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Determination of Cytokinins in Rice Root Exudates by High Performance Liquid Chromatography(HPLC)

SHEN Bo ^{1; 2}; PING Xiao-fei ²; TANG Fu-bing ²; YAN Hong-lan²; WANG Xi ²

（ ¹Key Laboratory for Biochemistry and Molecular Biology; Hangzhou Normal College; Hangzhou 310012; China; ²State Key Laboratory of Rice Biology; China National Rice Research Institute; Hangzhou 310006; China）

Received:1900-01-01 Revised:1900-01-01 Online:2004-07-10 Published:2004-07-10

高压液相色谱法检测水稻根系伤流液中细胞分裂素类物质

沈波1;2; 平霄飞2; 汤富彬2; 颜红岚2;王熹2

1杭州师范学院生物化学与分子生物学重点实验室，浙江杭州 310012； 2中国水稻研究所水稻生物学国家重点实验室，浙江杭州310006

Abstract

Abstract: A high performance liquid chromatographic method had been established to determine cytokinin contents of root exudates in rice. This method was simple, reliable and accurate, and could be used for measuring zeatin, dihydrozeatin, dihydrozeatin riboside, and N₆ isopentenyladenine in rice root exudates.

Key words: high performance liquid chromatography, cytokinin, rice, methodology

摘要： 建立了应用高压液相色谱法检测水稻根系伤流液中细胞分裂素类物质的方法。该法具有灵敏、可靠、准确的特点，可检测到玉米素（Z）、二氢玉米素（diHZ）、二氢玉米素核苷（diHZR）和异戊烯基腺嘌呤(iP)4种细胞分裂素。

关键词: 高压液相色谱, 细胞分裂素, 水稻, 方法

SHEN Bo ,PING Xiao-fei ,TANG Fu-bing ,YAN Hong-lan ,WANG Xi . Determination of Cytokinins in Rice Root Exudates by High Performance Liquid Chromatography(HPLC)[J]. Chinese Journal of Rice Science.

沈波, 平霄飞, 汤富彬, 颜红岚,王熹. 高压液相色谱法检测水稻根系伤流液中细胞分裂素类物质[J]. 中国水稻科学.

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Determination of Cytokinins in Rice Root Exudates by High Performance Liquid Chromatography(HPLC)

高压液相色谱法检测水稻根系伤流液中细胞分裂素类物质

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