Abstract: By using rice plasmid as materials, the feature of MegaBACE 1000 DNA analysis system and the key parameters affecting the quality of DNA sequencing were analyzed. It showed that the quality of plasmid DNA was the key parameter affecting sequencing, and the homogeneity of the sequencing reaction products in the same plate was critical to improve efficiency of high-throughput DNA sequencing.

Key words: plasmid DNA, sequencing, rice, factor

摘要： 以水稻cDNA克隆为材料，讨论了应用MegaBACE 1000 DNA分析系统进行大规模质粒测序的特点,并着重对影响大规模质粒测序效果的相关因素进行了分析,认为质粒DNA的质量是影响测序效果的决定性因素,而同一批测序反应中保证各反应终产物的均一性是提高大规模测序成功率的关键。

关键词: 质粒DNA, 测序, 水稻, 影响因素