Chinese Journal OF Rice Science

Chinese Journal of Rice Science

• 研究简报 • Previous Articles     Next Articles

Clustering of Major Genes Conferring Blast Resistance in Rice Cultivar Gumei 2

WU Jian-li1; 3; 4; CHAI R ong-yao2; FAN Ye-yang1; LI De-bao3; ZHENG Kang-le1; Hei LEUNG4; ZHUANG Jie-yun1; *   

  1. 1State Key Laboratory of Rice Biology; Chinese National Center for Rice Improvement; China National Rice Research Institute; Hangzhou 310006; China; 2Plant Protection Institute; Zhejiang Academy of Agricultural Sciences; Ha ngzhou 310021; 3Institute of Biotechnology; Zhejiang University; Hangz hou 310029; 4 Entomology and Plant Pathology Division; International Rice Research Institute, Manila, Philippines.
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-11-10 Published:2004-11-10

抗稻瘟病水稻材料谷梅2号中主效抗稻瘟病基因的成簇分布

吴建利1,3,4; 柴荣耀2; 樊叶杨1; 李德葆3; 郑康乐1; Hei LEUNG4; 庄杰云1,*   

  1. 1中国水稻研究所 国家水稻改良中心, 水稻生物学国家重点实验室， 浙江 杭州 310006； 2浙江省农业科学院 植物保护研究所， 浙江 杭州 310021； 3浙江大学 生物技术研究所， 浙江 杭州 310029； 4国际水稻研究所，菲律宾 马尼拉; *通讯联系人， E-mail:jz1803@hzcnc.com

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Abstract: By using 304 recombinant inbred lines derived fro m indica rice cross Zhong 156/Gumei 2, a linkage map consisting of 177 marker loci and covering 12 rice chromosomes was constructed and employed for mapping genes conferring blast resistance in rice. Genomic location of gene Pi25(t) conferring neck blast resistance to Chinese isolate 92-183 (race ZC15) was verified to be located between markers A7 and RG456 on chromosome 6, with genetic distances of 1.7 cM and 1.5 cM to A7 and RG456, respe ctively. Leaf blast resistance of Gumei 2 to Philippine isolate Ca89 (lineage 4 ) was found to be controlled by a single gene. The gene tentatively designated as Pi26(t) was located between markers B10 and R674 on chromosome 6, with genetic distances of 5.7 cM and 25.8 cM to B10 and R674 , respectively. Resistant alleles at both gene loci were derived from Gumei 2, indicating an existence of resistance gene clusters in Gumei 2.

Key words: blast resistance, molecular mapping, gene cluster, rice

摘要： 应用由籼稻组合中156/谷梅2号衍生的304个重组自交系，构建了由177个标记组成、覆盖12条水稻染色体的连锁图谱，定位控制水稻稻瘟病抗性的主效基因。前期定位的控制对中国稻瘟菌菌系92183（小种ZC15）穗瘟抗性的基因Pi25(t)的位置得到进一步确认，位于第6染色体标记A7和RG456之间，与A7和RG456的遗传距离分别为1.7和15 cM；发现群体对菲律宾稻瘟菌菌系Ca89（4谱系）的叶瘟抗性由单基因控制，将该暂命名为Pi26(t)的基因定位于第6染色体标记B10和R674之间，与B10和R674的遗传距离分别为5.7和25.8 cM。两个基因座位上的抗病等位基因均来源于谷梅2号，表明谷梅2号中存在控制水稻稻瘟病抗性的基因簇。

关键词: 稻瘟病抗性, 分子定位, 基因簇