Abstract: A new method to detect the first base of splice donor of Wx intron 1 in rice was reported, and some indica rice samples from Guangdong Province were analyzed using this method. The results showed that this new method is credible, easier and cheaper than the usual PCR-Acc Ⅰ method. It meets the requirement for amylose content selection in rice breeding.

Key words: rice, amylose content, polymerase chain reaction, research methodology

摘要： 报道了一种利用PCR快速检测水稻蜡质基因第一内含子剪接供体+1位碱基的方法，并用这种方法分析了一批广东籼稻材料。结果显示，该方法简便易行，结果可靠，可广泛用于育种筛选中的直链淀粉含量选择。

关键词: 水稻, 直链淀粉含量, 聚合酶链式反应, 研究方法