Abstract: A technique for inoculating with Pyricularia oryzae Cav. for neck blast resistance screening was studied. Inoculation is carried out by spraying or smearing the mixture 1-2×10⁵ conidia/ml suspended in 2% carboxymethyl cellulose (CMC) onto the in vitro rice panicle with a brush. The new method is suitale for inoculation at 0-10 days after heading, especially at the 0-3 days after heading. The disease index after inoculation is injection > smearing > spraying two times > spraying one times > around neck with cotton soaked in spore suspension. The method requires only a small amount of spore suspension and gives a high infection frequency, when smearing inoculation was used. It is an effective method for studying the resistance resource screening and resistance inheritance of neck blast.

Key words: Artificial inoculation, In vitro rice panicle, Neck blast, Pyricularia oryzae

关键词: 人工接种, 离体稻穗, 穗颈瘟, 稻瘟病