Abstract: Strain 2539B₁ of rice blast fungus (Pyricularia oryzae) was selected as recipient to develop the transformation system for the fungus. Enough protoplasts were obtained by digestion of the fungal cell wall with the lyric enzymes of Novozyme-234 or that made in China. The efficiencies were similar for preparation of fungal protoplasts by using the domestic lytic enzyme or Novozyme-234, and the regenertion abilities were strong for protoplasts prepared by the two enzymes. The density of 10⁸ cells protoplasts per millilitre can be gained by digesting gram fresh mycelium for 2 hours with above two lytic enzymes. The protoplasts prepared possess similar characters with the condia of Pyricularia oryzae.

Key words: Lytic enzyme, Protoplast preparation, Pyricularia oryzae

摘要： 为建立稻瘟病菌转化体系，选用稻瘟病菌2539B为受体，用Novozyme-234或国产裂解酶分别消化该菌细胞壁，都获得了足够数量的原生质体，并具再生菌的能力，其效率大体相近。上述两种裂解酶按1 g鲜重菌丝加50 mg酶量，消化2 h即可获得4．65×10⁸细胞／mL密度的原生质体,制备的原生质体和稻瘟病菌分生孢子具有相似的习性。

关键词: 稻瘟病, 裂解酶, 原生质体, 制备, 水稻