The Detection of Xanthomonas campestris pv. oryzae with the McAb-ELISA after Multiplication

Chinese Journal of Rice Science

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The Detection of Xanthomonas campestris pv. oryzae with the McAb-ELISA after Multiplication

Li Qingxian; Li Shiwen; Gao Jinliang; Hu Guanggan; Zhu Hua; Huang Benli

Received:1900-01-01 Revised:1900-01-01 Online:1992-06-05 Published:1992-06-05

用单克隆抗体酶联免疫吸附测定法检测水稻种子上的白叶枯病菌

李清铣; 刘斯文; 高锦梁; 胡广淦; 朱华; 黄奔立

江苏农学院，扬州225001

Abstract

Abstract: A double antibodies sandwich ELISA with the monoclonal antibody as the second antibody for the rapid detection of Xanthomonas campestris pv. oryzae in rice seeds was developed. The bacterial from husk of the seed samples were shake-cultured in sterile liquid medium for 48 h at 25℃, and followed by ELISA detection. 126 seed samples from Jiangsu and Zhejiang were tested, 93% of seed samples from infected rice were positive.

Key words: ELISA test, Monoclonal antibody, seed, Xanthomonas campestris pv. oryzae

关键词: 种子, 水稻, 白叶枯病, 单克隆抗体, 免疫吸附测定法

Li Qingxian,Li Shiwen,Gao Jinliang,Hu Guanggan,Zhu Hua,Huang Benli. The Detection of Xanthomonas campestris pv. oryzae with the McAb-ELISA after Multiplication [J]. Chinese Journal of Rice Science.

李清铣, 刘斯文, 高锦梁, 胡广淦, 朱华, 黄奔立. 用单克隆抗体酶联免疫吸附测定法检测水稻种子上的白叶枯病菌[J]. 中国水稻科学.

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The Detection of Xanthomonas campestris pv. oryzae with the McAb-ELISA after Multiplication

用单克隆抗体酶联免疫吸附测定法检测水稻种子上的白叶枯病菌

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