Effects of Nitrogen Application Rate and Transplanting Density on Yield Formation of Small Seed Hybrid Rice

doi:10.16819/j.1001-7216.2024.231116

Abstract

Abstract:

【Objective】 To elucidate the yield formation characteristics of small-seed hybrid rice.【Method】 Zhuoliangyou 0985 (small-seed hybrid rice variety) and Fengliangyou 4 (control variety) were utilized as materials for a field experiment at varying nitrogen application rates (0, N0; 180 kg/hm², N1; 210 kg/hm², N2) and densities (26.7 cm×20.0 cm, D1; 20.0 cm×20.0 cm, D2; 20.0 cm×16.7 cm, D3)【Result】 The leaf area index (LAI) and leaf SPAD values of the two varieties increased with nitrogen application rate, with no significant difference between N1 and N2, but significantly higher than N0 treatment. The dry matter output rate of stems and sheaths decreased with rising nitrogen application, while leaf matter output rate followed the opposite trend. Grain yield of both varieties increased with increasing nitrogen application, with no significant difference between N1 and N2. Among different density treatments, D2 generally yielded higher results. Under N0 treatment, Zhuoliangyou 0985 had significantly higher yield than the control variety, while under nitrogen application conditions, both varieties had equivalent yields. 【Conclusion】 The yield formation characteristics of Zhuoliangyou 0985 include smaller leaf area, more grains per panicle, larger sink-source ratio, noticeable photosynthetic advantages during mid to late grain filling stages, higher post-flowering substance accumulation and transport rate of stem and leaf substances, higher harvest index, and increased yield under nitrogen-deficient conditions. In the context of this experiment, N1D2 (180 kg/hm², 20cm×20cm) emerged as the optimal treatment for Zhuoliangyou 0985.

Key words: small-seed hybrid rice, nitrogen-density interaction, yield formation, material transport, source sink properties

摘要：

【目的】 明确小粒型杂交稻的产量形成特性。【方法】 以卓两优0985(小粒型杂交稻)和丰两优4号(对照)为材料，在不同施氮量(0，N0；180 kg/hm²，N1；210 kg/hm²，N2)和密度(26.7 cm×20.0 cm，D1；20.0 cm×20.0 cm，D2；20.0 cm×16.7 cm，D3)条件下开展大田试验。【结果】 两品种LAI、叶片SPAD值随施氮量增大而提高，N1与N2差异不显著，但显著高于N0处理；两品种茎鞘物质输出率随施氮量增大而下降，而叶片物质输出率表现相反；两品种产量随施氮量增加而提高，但N1与N2间差异不显著，不同密度处理下一般以D2处理较高；N0处理下卓两优0985产量显著高于对照品种，而施氮条件下两品种产量相当。【结论】 卓两优0985产量形成特性为叶面积较小、穗粒数较多、库源比较大，灌浆中后期光合优势明显，花后物质积累量与茎叶物质转运率、经济系数较高，不施氮条件下具有较高产量等。本研究条件下，卓两优0985以N1D2(180 kg/hm²、20 cm×20 cm)为最佳处理。

关键词: 小粒型杂交稻, 氮密互作, 产量形成, 物质转运, 源库特性

LÜ Zhou, YI Binghuai, CHEN Pingping, ZHOU Wenxin, TANG Wenbang, YI Zhenxie. Effects of Nitrogen Application Rate and Transplanting Density on Yield Formation of Small Seed Hybrid Rice[J]. Chinese Journal OF Rice Science, 2024, 38(4): 422-436.

吕宙, 易秉怀, 陈平平, 周文新, 唐文帮, 易镇邪. 施氮量与移栽密度对小粒型杂交水稻产量形成的影响[J]. 中国水稻科学, 2024, 38(4): 422-436.

Figures/Tables 9

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[37]	周江明, 赵琳, 董越勇, 徐进, 边武英, 毛杨仓, 章秀福. 氮肥和栽植密度对水稻产量及氮肥利用率的影响[J]. 植物营养与肥料学报, 2010, 16(2): 274-281.
	Zhou J M, Zhao L, Dong Y Y, Xu J, Bian W Y, Mao Y C, Zhang X F. Nitrogen and transplanting density interactions on the rice yield and N use rate[J]. Journal of Plant Nutrition and Fertilizers, 2010, 16(2): 274-281. (in Chinese with English abstract)
[38]	胡杨, 段斌, 何世界, 扶定, 方玲, 李慧龙, 霍二伟, 沈光辉. 基本苗数和氮肥运筹对深两优19产量及稻米品质的影响[J]. 安徽农业科学, 2023, 51(19): 23-26+40.
	Hu Y, Duan B, He S J, Fu D, Fang L, Li H L, Huo E W, Shen G H. Effects of basic seedling number and nitrogen fertilizer management on yield and quality of Shenliangyou 19[J]. Journal of Anhui Agricultural Sciences, 2019, 51(19): 23-26+40. (in Chinese with English abstract)
[39]	金峰, 邵玺文, 李彦利, 耿艳秋, 华霜, 王帅, 朱明霞, 韩康顺, 时羽. 株行距配置对吉林省水稻生长发育动态及物质生产的影响[J]. 东北农业科学, 2016, 41(5): 17-23.
	Jin F, Shao X W, Li Y L, Geng Y Q, Hua S, Wang S, Zhu M X, Han K S, Shi Y. Effects of row-spacing on dynamics of growth and development, and matter production of rice in Jilin province[J]. Journal of Northeast Agricultural Sciences, 2016, 41(5): 17-23. (in Chinese with English abstract)
[40]	方文英, 陈佳麒, 沈兴连, 莫红华, 刘海威, 纪国成. 氮肥调控运筹实现甬优12超15 t·hm^-2的性状特征与技术途径[J]. 浙江农业科学, 2022, 63(6): 1218-1222.
	Fang W Y, Chen J Q, Shen X L, Mo H H, Liu H W, Ji G C. Characteristics and technical approaches of nitrogen control and management to achieveyield exceeding 15 t·hm^-2 of Yongyou 12[J]. Journal of Zhejiang Agricultural Sciences, 2022, 63(6): 1218-1222. (in Chinese)
[41]	李圆圆, 何平, 茅桁. 稻田水肥管理研究进展及思考[J]. 排灌机械工程学报, 2023, 41(8): 825-832.
	Li Y Y, He P, Mao H. Research progress and thinking on water and fertilizer management inrice field[J]. Journal of Drainage and Irrigation Machinery Engineering, 2023, 41(8): 825-832. (in Chinese with English abstract)

处理 Treatment	卓两优0985 Zhuoliangyou 0985
	2022					2023
	孕穗期 Booting		灌浆中期 Mid-filling	成熟期 Maturity		孕穗期 Booting	灌浆中期 Mid-filling		成熟期 Maturity
N0D1	3.72±0.16 bc		3.27±0.19 b	2.36±0.18 c		3.88±0.17 b	3.28±0.16 c		2.44±0.14 c
N0D2	4.05±0.20 c		3.44±0.18 b	2.65±0.17 c		4.06±0.14 b	3.54±0.13 bc		2.67±0.12 c
N0D3	3.48±0.18 b		2.93±0.16 b	2.27±0.15 c		3.52±0.15 b	3.98±0.14 b		2.31±0.12 c
平均 Mean	3.75		3.21	2.43		3.82	3.60		2.47
N1D1	6.85±0.17 a		5.92±0.17 a	5.02±0.15 b		6.94±0.21 a	6.02±0.16 a		5.12±0.15 b
N1D2	7.02±0.22 a		6.12±0.21 a	5.24±0.19 a		7.21±0.16 a	6.21±0.16 a		5.26±0.20 a
N1D3	6.77±0.20 a		5.86±0.19 a	4.88±0.20 b		6.78±0.17 a	5.92±0.16 a		4.89±0.16 b
平均Mean	6.88		5.97	5.05		6.98	6.05		5.09
N2D1	6.97±0.19 a		6.15±0.13 a	5.23±0.13 a		7.02±0.15 a	6.21±0.16 a		5.26±0.18 a
N2D2	7.22±0.14 a		6.33±0.21 a	5.51±0.14 a		7.36±0.15 a	6.38±0.14 a		5.64±0.19 a
N2D3	6.82±0.21 a		6.02±0.20 a	5.12±0.15 ab		6.94±0.19 a	6.04±0.19 a		5.21±0.18 ab
平均Mean	7.00		6.17	5.29		7.11	6.21		5.37
F值 F value	72.82		60.81	71.54		93.44	67.32		73.43
施氮量 N rate(N)	**		**	**		**	**		**
密度 Density(D)	*		*	*		**	ns		*
施氮量×密度 N×D	ns		ns	ns		ns	ns		ns
处理 Treatment	丰两优4号 Fengliangyou 4
	2022					2023
	孕穗期 Booting	灌浆中期 Mid-filling			成熟期 Maturity	孕穗期 Booting	灌浆中期 Mid-filling	成熟期 Maturity
N0D1	4.12±0.13 c	2.98±0.16 cd			2.26±0.16 c	4.19±0.15 cd	2.99±0.16 c	2.31±0.13 d
N0D2	4.45±0.10 c	3.39±0.16 c			2.43±0.14 c	4.56±0.17 c	3.41±0.15 c	2.51±0.12 d
N0D3	3.68±0.14 d	2.83±0.16 d			2.12±0.13 c	3.84±0.14 d	2.89±0.16 cd	2.24±0.14 d
平均Mean	4.08	3.07			2.27	4.20	3.10	2.35
N1D1	7.55±0.16 ab	6.77±0.17 ab			5.94±0.15 ab	7.66±0.13 ab	6.81±0.19 ab	6.04±0.11 abc
N1D2	7.89±0.16 a	6.98±0.15 a			6.08±0.16 a	7.96±0.17 ab	7.13±0.19 a	6.24±0.12 ab
N1D3	7.31±0.16 b	6.48±0.16 b			5.63±0.14 b	7.42±0.13 b	6.51±0.17 b	5.71±0.15 c
平均Mean	7.58	6.74			5.88	7.68	6.82	6.00
N2D1	7.62±0.16 ab	6.81±0.15 ab			6.02±0.17 ab	7.69±0.16 ab	6.92±0.19 ab	6.04±0.14 abc
N2D2	7.96±0.19 a	7.14±0.15 a			6.28±0.12 a	8.03±0.16 a	7.24±0.18 a	6.36±0.12 a
N2D3	7.55±0.15 ab	6.72±0.16 ab			5.94±0.17 ab	7.58±0.15 b	6.81±0.18 a	5.89±0.14 bc
平均Mean	7.71	6.89			6.08	7.77	6.99	6.10
F值 F value	138.80	142.74			144.74	137.30	121.58	204.87
施氮量 N rate(N)	**	**			**	**	**	**
密度 Density(D)	*	*			*	*	ns	ns
施氮量×密度 N×D	ns	ns			ns	ns	ns	ns

处理 Treatment	卓两优0985 Zhuoliangyou 0985
	2022					2023
	孕穗期 Booting		灌浆中期 Mid-filling	成熟期 Maturity		孕穗期 Booting	灌浆中期 Mid-filling		成熟期 Maturity
N0D1	3.72±0.16 bc		3.27±0.19 b	2.36±0.18 c		3.88±0.17 b	3.28±0.16 c		2.44±0.14 c
N0D2	4.05±0.20 c		3.44±0.18 b	2.65±0.17 c		4.06±0.14 b	3.54±0.13 bc		2.67±0.12 c
N0D3	3.48±0.18 b		2.93±0.16 b	2.27±0.15 c		3.52±0.15 b	3.98±0.14 b		2.31±0.12 c
平均 Mean	3.75		3.21	2.43		3.82	3.60		2.47
N1D1	6.85±0.17 a		5.92±0.17 a	5.02±0.15 b		6.94±0.21 a	6.02±0.16 a		5.12±0.15 b
N1D2	7.02±0.22 a		6.12±0.21 a	5.24±0.19 a		7.21±0.16 a	6.21±0.16 a		5.26±0.20 a
N1D3	6.77±0.20 a		5.86±0.19 a	4.88±0.20 b		6.78±0.17 a	5.92±0.16 a		4.89±0.16 b
平均Mean	6.88		5.97	5.05		6.98	6.05		5.09
N2D1	6.97±0.19 a		6.15±0.13 a	5.23±0.13 a		7.02±0.15 a	6.21±0.16 a		5.26±0.18 a
N2D2	7.22±0.14 a		6.33±0.21 a	5.51±0.14 a		7.36±0.15 a	6.38±0.14 a		5.64±0.19 a
N2D3	6.82±0.21 a		6.02±0.20 a	5.12±0.15 ab		6.94±0.19 a	6.04±0.19 a		5.21±0.18 ab
平均Mean	7.00		6.17	5.29		7.11	6.21		5.37
F值 F value	72.82		60.81	71.54		93.44	67.32		73.43
施氮量 N rate(N)	**		**	**		**	**		**
密度 Density(D)	*		*	*		**	ns		*
施氮量×密度 N×D	ns		ns	ns		ns	ns		ns
处理 Treatment	丰两优4号 Fengliangyou 4
	2022					2023
	孕穗期 Booting	灌浆中期 Mid-filling			成熟期 Maturity	孕穗期 Booting	灌浆中期 Mid-filling	成熟期 Maturity
N0D1	4.12±0.13 c	2.98±0.16 cd			2.26±0.16 c	4.19±0.15 cd	2.99±0.16 c	2.31±0.13 d
N0D2	4.45±0.10 c	3.39±0.16 c			2.43±0.14 c	4.56±0.17 c	3.41±0.15 c	2.51±0.12 d
N0D3	3.68±0.14 d	2.83±0.16 d			2.12±0.13 c	3.84±0.14 d	2.89±0.16 cd	2.24±0.14 d
平均Mean	4.08	3.07			2.27	4.20	3.10	2.35
N1D1	7.55±0.16 ab	6.77±0.17 ab			5.94±0.15 ab	7.66±0.13 ab	6.81±0.19 ab	6.04±0.11 abc
N1D2	7.89±0.16 a	6.98±0.15 a			6.08±0.16 a	7.96±0.17 ab	7.13±0.19 a	6.24±0.12 ab
N1D3	7.31±0.16 b	6.48±0.16 b			5.63±0.14 b	7.42±0.13 b	6.51±0.17 b	5.71±0.15 c
平均Mean	7.58	6.74			5.88	7.68	6.82	6.00
N2D1	7.62±0.16 ab	6.81±0.15 ab			6.02±0.17 ab	7.69±0.16 ab	6.92±0.19 ab	6.04±0.14 abc
N2D2	7.96±0.19 a	7.14±0.15 a			6.28±0.12 a	8.03±0.16 a	7.24±0.18 a	6.36±0.12 a
N2D3	7.55±0.15 ab	6.72±0.16 ab			5.94±0.17 ab	7.58±0.15 b	6.81±0.18 a	5.89±0.14 bc
平均Mean	7.71	6.89			6.08	7.77	6.99	6.10
F值 F value	138.80	142.74			144.74	137.30	121.58	204.87
施氮量 N rate(N)	**	**			**	**	**	**
密度 Density(D)	*	*			*	*	ns	ns
施氮量×密度 N×D	ns	ns			ns	ns	ns	ns

处理 Treatment	卓两优0985 Zhuoliangyou 0985
	2022					2023
	孕穗期 Booting		灌浆中期 Mid-filling		成熟期 Maturity	孕穗期 Booting		灌浆中期 Mid-filling	成熟期 Maturity
N0D1	29.05±0.891 d		27.11±0.92 b		22.51±0.52 b	29.14±0.77 d		27.58±0.52 b	22.54±0.49 b
N0D2	31.22±0.60 cd		28.24±0.65 b		23.01±0.17 b	30.17±0.57 cd		28.48±0.50 b	22.63±0.47 b
N0D3	32.65±0.53 c		28.69±0.66 b		23.71±0.70 b	31.22±0.58 c		28.65±0.57 b	23.15±0.57 b
平均Mean	30.97		28.01		23.08	30.18		28.24	22.77
N1D1	42.66±0.78 b		42.56±0.77 a		34.66±0.62 a	42.51±0.59 b		42.66±0.59 a	34.88±0.50 a
N1D2	42.55±0.70 b		43.54±0.82 a		35.62±0.58 a	42.57±0.56 b		44.15±0.50 a	35.21±0.56 a
N1D3	43.02±0.48 b		43.35±0.71 a		36.05±0.55 a	43.15±0.54 b		43.68±0.83 a	35.24±0.63 a
平均Mean	42.74		43.15		35.44	42.74		43.50	35.11
N2D1	42.57±0.42 b		43.17±0.54 a		34.68±0.53 a	42.68±0.48 b		43.12±0.49 a	35.14±0.40 a
N2D2	44.79±0.77 ab		43.31±0.75 a		35.47±0.57 a	43.58±0.51 ab		44.02±0.48 a	35.27±0.52 a
N2D3	46.25±0.60 a		43.44±0.66 a		36.24±0.62 a	45.15±0.49 a		43.94±0.56 a	36.12±0.62 a
平均Mean	44.54		43.31		35.46	43.80		43.69	35.51
F值 F value	101.58		109.7		128.83	133.82		181.67	137.97
施氮量 N rate(N)	**		**		**	**		**	**
密度 Density(D)	**		ns		*	**		ns	ns
施氮量×密度N×D	ns		ns		ns	ns		ns	ns
处理 Treatment		丰两优4号 Fengliangyou 4
		2022				2023
		孕穗期 Booting	灌浆中期 Mid-filling	成熟期 Maturity		孕穗期 Booting	灌浆中期 Mid-filling		成熟期 Maturity
N0D1		30.25±0.66 c	27.44±0.52 b	18.77±0.49 b		30.25±0.43 b	27.54±0.47 c		18.89±0.50 b
N0D2		30.85±0.47 c	27.67±0.31 b	18.66±0.51 b		30.87±0.30 b	27.68±0.40 c		18.47±0.30 b
N0D3		30.25±0.51 c	27.75±0.71 b	19.02±0.59 b		31.25±0.49 b	27.99±0.51 c		19.35±0.52 b
平均Mean		30.45	27.62	18.82		30.79	27.74		18.90
N1D1		44.58±0.58 b	41.29±0.55 a	27.55±0.53 a		44.68±0.52 a	40.28±0.40 b		27.68±0.40 a
N1D2		44.21±0.54 b	41.05±0.85 a	28.01±0.41 a		44.98±0.53 a	40.98±0.34 ab		28.42±0.58 a
N1D3		45.04±0.54 ab	41.25±0.48 a	28.61±0.60 a		45.24±0.44 a	41.25±0.51 ab		28.69±0.54 a
平均Mean		44.61	41.20	28.06		44.97	40.84		28.26
N2D1		45.02±0.57 ab	41.78±0.51 a	27.42±0.57 a		43.98±0.43 a	41.58±0.42 a		28.01±0.46 a
N2D2		45.41±0.52 ab	40.87±0.59 a	28.11±0.36 a		44.68±0.44 a	41.27±0.42 ab		27.59±0.41 a
N2D3		46.25±0.60 a	41.55±0.55 a	28.67±0.52 a		45.29±0.40 a	41.65±0.42 a		28.64±0.47 a
平均Mean		45.56	41.40	28.07		44.65	41.50		28.08
F值 F value		179.44	144.07	82.44		244.52	239.43		97.53
施氮量 N rate(N)		**	**	**		**	**		**
密度 Density(D)		ns	ns	ns		ns	ns		ns
施氮量×密度N×D		ns	ns	ns		ns	ns		ns

处理 Treatment	卓两优0985 Zhuoliangyou 0985
	2022					2023
	孕穗期 Booting		灌浆中期 Mid-filling		成熟期 Maturity	孕穗期 Booting		灌浆中期 Mid-filling	成熟期 Maturity
N0D1	29.05±0.891 d		27.11±0.92 b		22.51±0.52 b	29.14±0.77 d		27.58±0.52 b	22.54±0.49 b
N0D2	31.22±0.60 cd		28.24±0.65 b		23.01±0.17 b	30.17±0.57 cd		28.48±0.50 b	22.63±0.47 b
N0D3	32.65±0.53 c		28.69±0.66 b		23.71±0.70 b	31.22±0.58 c		28.65±0.57 b	23.15±0.57 b
平均Mean	30.97		28.01		23.08	30.18		28.24	22.77
N1D1	42.66±0.78 b		42.56±0.77 a		34.66±0.62 a	42.51±0.59 b		42.66±0.59 a	34.88±0.50 a
N1D2	42.55±0.70 b		43.54±0.82 a		35.62±0.58 a	42.57±0.56 b		44.15±0.50 a	35.21±0.56 a
N1D3	43.02±0.48 b		43.35±0.71 a		36.05±0.55 a	43.15±0.54 b		43.68±0.83 a	35.24±0.63 a
平均Mean	42.74		43.15		35.44	42.74		43.50	35.11
N2D1	42.57±0.42 b		43.17±0.54 a		34.68±0.53 a	42.68±0.48 b		43.12±0.49 a	35.14±0.40 a
N2D2	44.79±0.77 ab		43.31±0.75 a		35.47±0.57 a	43.58±0.51 ab		44.02±0.48 a	35.27±0.52 a
N2D3	46.25±0.60 a		43.44±0.66 a		36.24±0.62 a	45.15±0.49 a		43.94±0.56 a	36.12±0.62 a
平均Mean	44.54		43.31		35.46	43.80		43.69	35.51
F值 F value	101.58		109.7		128.83	133.82		181.67	137.97
施氮量 N rate(N)	**		**		**	**		**	**
密度 Density(D)	**		ns		*	**		ns	ns
施氮量×密度N×D	ns		ns		ns	ns		ns	ns
处理 Treatment		丰两优4号 Fengliangyou 4
		2022				2023
		孕穗期 Booting	灌浆中期 Mid-filling	成熟期 Maturity		孕穗期 Booting	灌浆中期 Mid-filling		成熟期 Maturity
N0D1		30.25±0.66 c	27.44±0.52 b	18.77±0.49 b		30.25±0.43 b	27.54±0.47 c		18.89±0.50 b
N0D2		30.85±0.47 c	27.67±0.31 b	18.66±0.51 b		30.87±0.30 b	27.68±0.40 c		18.47±0.30 b
N0D3		30.25±0.51 c	27.75±0.71 b	19.02±0.59 b		31.25±0.49 b	27.99±0.51 c		19.35±0.52 b
平均Mean		30.45	27.62	18.82		30.79	27.74		18.90
N1D1		44.58±0.58 b	41.29±0.55 a	27.55±0.53 a		44.68±0.52 a	40.28±0.40 b		27.68±0.40 a
N1D2		44.21±0.54 b	41.05±0.85 a	28.01±0.41 a		44.98±0.53 a	40.98±0.34 ab		28.42±0.58 a
N1D3		45.04±0.54 ab	41.25±0.48 a	28.61±0.60 a		45.24±0.44 a	41.25±0.51 ab		28.69±0.54 a
平均Mean		44.61	41.20	28.06		44.97	40.84		28.26
N2D1		45.02±0.57 ab	41.78±0.51 a	27.42±0.57 a		43.98±0.43 a	41.58±0.42 a		28.01±0.46 a
N2D2		45.41±0.52 ab	40.87±0.59 a	28.11±0.36 a		44.68±0.44 a	41.27±0.42 ab		27.59±0.41 a
N2D3		46.25±0.60 a	41.55±0.55 a	28.67±0.52 a		45.29±0.40 a	41.65±0.42 a		28.64±0.47 a
平均Mean		45.56	41.40	28.07		44.65	41.50		28.08
F值 F value		179.44	144.07	82.44		244.52	239.43		97.53
施氮量 N rate(N)		**	**	**		**	**		**
密度 Density(D)		ns	ns	ns		ns	ns		ns
施氮量×密度N×D		ns	ns	ns		ns	ns		ns

处理 Treatment	2022		2023
处理 Treatment	卓两优0985 Zhuoliangyou 0985	丰两优4号 Fengliangyou 4	卓两优0985 Zhuoliangyou 0985	丰两优4号 Fengliangyou 4
N0D1	18.22±0.30 c	16.25±0.46 c	18.46±0.29 c	16.56±0.27 c
N0D2	18.25±0.33 c	16.87±0.31 c	18.49±0.30 c	16.74±0.24 c
N0D3	18.67±0.42 c	16.59±0.41 c	18.52±0.20 c	16.68±0.29 c
平均Mean	18.38	16.57	17.49	16.66
N1D1	23.86±0.45 b	20.44±0.46 b	23.88±0.30 b	20.65±0.25 b
N1D2	24.58±0.34 ab	21.24±0.41 ab	23.96±0.45 ab	20.87±0.27 ab
N1D3	24.98±0.40 ab	21.41±0.28 ab	24.15±0.44 ab	21.17±0.39 ab
平均Mean	24.47	21.03	24.00	20.90
N2D1	23.99±0.26 ab	20.46±0.29 ab	23.98±0.23 b	20.55±0.29 b
N2D2	24.66±0.44 ab	21.31±0.32 ab	24.54±0.39 ab	21.54±0.24 a
N2D3	25.06±0.45 a	21.54±0.30 a	24.98±0.31 a	21.69±0.42 a
平均Mean	24.57	21.10	24.50	21.26
F值 F value	63.49	39.064	72.40	54.82
施氮量 N rate(N)	**	**	**	**
密度 Density(D)	ns	ns	ns	ns
施氮量×密度 N×D	ns	ns	ns	ns