Genetic Dissection of Broad Spectrum Resistance of the Rice Maintainer Ganxiang B

doi:10.16819/j.1001-7216.2023.221105

Abstract

Abstract:

【Objective】 Ganxiang B is a WA type rice maintainer line developed by the Rice Research Institute of Jiangxi Academy of Agricultural Sciences. It shows broad spectrum resistance against rice blast in Jiangxi, Fujian, Guizhou, Hubei, and Zhejiang Provinces. Genetic analysis of blast resistance loci in Ganxiang B will contribute to the future work for blast resistance breeding. 【Method】 The rice blast resistance loci in Ganxiang B were analyzed by genome comparison between resistant and susceptible lines, marker detection of rice blast resistance locus, resistance gene sequencing, and phenotypic identification of segment substitution lines. 【Results】 There were resistance genes Pish, Pia, and Pita in Ganxiang B. After introgressed with Pish, the blast resistance of highly susceptible line was significantly enhanced. However, after introgressed with Pita or Pia, the resistance was slightly enhanced or remained unchanged.【Conclusion】 Ganxiang B carries several blast resistance genes. Among them, Pish is the main resistant gene, showing broad resistance to rice blast.

Key words: rice blast, resistance, genetic dissection

摘要：

【目的】 赣香B是江西省农业科学院水稻研究所育成的水稻野败型保持系，在江西、福建、贵州、湖北和浙江等多地都表现较强的稻瘟病广谱抗性。对赣香B的稻瘟病抗性位点的遗传解析，有助于今后稻瘟病抗性育种工作。【方法】 通过抗感株系基因组比较、稻瘟病抗性位点标记检测、抗性位点测序和片段代换系表型鉴定，分析赣香B中的稻瘟病抗性基因位点。【结果】 赣香B中含稻瘟病抗性基因Pish、Pia和Pita。在稻瘟病高感株系中导入Pish后，抗性得到显著增强；而导入Pita或者Pia后，抗性略微增强或者没有显著效果。【结论】 赣香B携带多个稻瘟病抗性基因，其中，Pish是主效抗性基因，表现出广谱稻瘟病抗性。

关键词: 稻瘟病, 抗性, 遗传解析

CHEN Mingliang, XIONG Wentao, SHEN Yumin, XIONG Huanjin, LUO Shiyou, WU Xiaoyan, HU Lanxiang, XIAO Yeqing. Genetic Dissection of Broad Spectrum Resistance of the Rice Maintainer Ganxiang B[J]. Chinese Journal OF Rice Science, 2023, 37(5): 470-477.

陈明亮, 熊文涛, 沈雨民, 熊焕金, 罗世友, 吴小燕, 胡兰香, 肖叶青. 广谱抗稻瘟病水稻保持系赣香B的抗性遗传解析[J]. 中国水稻科学, 2023, 37(5): 470-477.

Figures/Tables 6

Table 1. Molecular markers used in detection for resistance genes to rice blast in present study.

基因位点Gene locus	引物 Primer	前引物 Forward primer	后引物 Backward primer	文献Document
标记引物 Marker primer
Pit	LJT3	GAGAATATCTTATGACAAACTAG	CATCTACGTTCTAATCCCCCAACG	[19]
Pish	LInD1-746	AGATCGTGCACTACCGACAAGT	TCCCATTGCATGAGAGGTGGTT	[20]
Pik	CKM1	TGAGCTCAAGGCAAGAGTTGAGGA	TGTTCCAGCAACTCGATGAG	[21]
	CKM2	CAGTAGCTGTGTCTCAGAACTATG	AAGGTACCTCTTTTCGGCCAG	[21]
Pia	STS	CTTTTGAGCTTGATTGGTCTGC	CTATTGCACCAGAGGGACCAG	[7]
Pb1	M1	ATCAACGCTACCTTCCC	GTGCCATCACAATTTCTTC	[22]
Pib	dom	GAACAATGCCCAAACTTGAGA	GGGTCCACATGTCAGTGAGC	[7]
Pita	YL155/YL87	AGCAGGTTATAAGCTAGGCC	CTACCAACAAGTTCATCAAA	[23]
测序引物 Primer for sequencing
Pish	Pish-1	CACGCTTTCTTGTCTCTGCT	CTATATGAGCATGAGGCGCT	[24]
Pi37	Pi37-1	CCATCACCATCTGTTCTTAT	GGGTTCCACCATATATATACC	[24]
Pi64	Pi64-1	GTCCGGCTAGAATATCTTAG	ATATGTGCCAATCTTGGGGT	[24]
Pita	Pita2	TACTTTGCAGGGGATGAGGC	TGATTCAGGGTCACAACAATAACA	[25]

Fig. 1. Comparison of phenotype and genome between susceptible line IL5133 and Ganxiang B. A, Phenotypic comparison between susceptible line IL5133 and Ganxiang B. The line IL5133 developed serious panicle blast, and Ganxiang B didn’t. B, Genome comparison between line IL5133 and Ganxiang B. Blue indicates the same loci between IL5133 and Ganxiang B, and red indicates the different loci. The circle indicates the rice blast resistance loci differed in sequence.

Fig. 2. Validation of the molecular markers in Ganxiang B and its parents. Lanes 1 to 6 are Ganxiang B, IR58025B, Jin 2B, Jiang 2B, Xinlu B and 03B, respectively; The positive controls(CK) of each resistance locus from A to H are IRBLt-K59, Nipponbare, Tetep, Tetep, IRBLa-A, Zhendao 18, IRBLb-B and Huazhan, respectively.

Table 2. Distribution of the resistant genes to rice blast in Ganxiang B and its parents.

基因 Gene	染色体 Chromosome	赣香B Ganxiang B	IR58025B	金23B Jin 23B	江2B Jiang 2B	新露B Xinlu B	03B
Pish	1	+	-	-	+	-	+
Pia	11	+	+	+	-	+	+
Pik	11
CKM1		-	+	-	+	-	-
CKM2		+	+	+	+	+	+
Pita	12	+	-	-	+	-	-

Fig. 3. Resistance to panicle blast of lines with different resistance loci from Ganxiang B(GXB) at Jinggangshan City, Hunan Province. 1, GXB; 2, IL5133; 3, IL-Pia; 4, IL-Pik; 5, IL-Pita; 6, IL-Pia+Pita; 7, IL-Pia+Pik+Pita; 8, IL-Pish; 9, IL-Pia+Pish; 10, IL-Pish+Pita; 11, IL-Pia+Pish+Pita.

Fig. 4. Resistance of backcrossed ZJZ18 lines. A, Panicle blast of different lines; B, Incidence of rice neck blast of different lines.

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