Phenotypic Analysis and Fine Mapping of blg1(beak like grain 1), a Rice Mutant with Abnormal Palea and Lemma Development

doi:10.16819/j.1001-7216.2023.220506

Abstract

Abstract:

【Objective】 The development of rice floral organs is very important for the successful reproduction and yield of rice. Genetic mapping of genes related to floret organ development can improve the regulatory network of rice floret development, which has theoretical significance for rice breeding.【Method】A mutant blg1 with abnormal palea and lemma was isolated and identified from breeding materials. The floret morphology of blg1 and control line was observed. The mutant was hybridized with Dular to form an F₂ population, and then genetic analysis, fine mapping and candidate gene analysis were carried out.【Result】Phenotypic observation showed that the palea and lemma in blg1 could not closed normally and curved severely with smaller grains. Genetic analysis showed that the mutant phenotype was controlled by a pair of recessive nuclear genes. Through fine mapping, the objective gene was located between markers InD3 and InD5 at the end of the long arm on chromosome 5, with a physical distance of 50 kb, including eight Open Reading Frames. By candidate gene analysis, LOC_Os05g48760 may be a candidate gene for BLG1 gene.【Conclusion】BLG1 is a new gene controlling the development of palea and lemma in rice.

Key words: rice, palea and lemma, fine mapping

摘要：

【目的】水稻花器官的正常发育对水稻的成功繁殖和产量至关重要。对水稻花器官发育相关基因进行遗传定位，可以完善和丰富其花器官发育调控网络，对水稻育种具有指导意义。【方法】从育种材料中分离鉴定出一个内外稃发育异常的突变体blg1，对blg1突变体和对照株系花器官形态进行了观察。将该突变体与Dular杂交，构建F₂群体，对突变表型进行遗传分析，并对BLG1基因进行了精细定位和候选基因分析。【结果】表型观察显示，blg1突变体表现为内外稃不能正常闭合、稃间弯曲严重、籽粒变小等。遗传分析表明，blg1的突变性状由一对隐性核基因控制。通过精细定位，将目标基因定位于第5染色体长臂末端InD3和InD5两个标记之间，物理距离为50 kb，包含8个开放阅读框。通过分析，LOC_Os05g48760可能是BLG1基因的候选基因。【结论】BLG1是一个控制水稻内外稃发育的新基因。

关键词: 水稻, 内外稃, 精细定位

SHEN Yumin, CHEN Mingliang, XIONG Huanjin, XIONG Wentao, WU Xiaoyan, XIAO Yeqing. Phenotypic Analysis and Fine Mapping of blg1(beak like grain 1), a Rice Mutant with Abnormal Palea and Lemma Development[J]. Chinese Journal OF Rice Science, 2023, 37(3): 225-232.

沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232.

Figures/Tables 6

References 47

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分子标记 Marker	前引物 (5’-3’) Forward sequence (5’-3’)	后引物 (5’-3’) Reverse sequence (5’-3’)
S1	TAATTGCGGTCTCGTGCC	AACCACCTCAACTACTGCCG
S2	CGCCTGCAAAAAAGGTAGAG	GATCAAAGGAACCCCCGTAG
S3	GCCGGTAGATGCAGTATTGC	GTGCACAGTCACGCAGAAAG
S4	TGCTGTCTCCACTTCCCTTC	AAGGAGGCGTGCTTAACAGG
S5	CTTGTCTTGGCTTGGCTAGG	ATCCACGCAGATAGACCTGC
InD1	AAAACGATCATAGTTGCTTGC	GGAGTTCGGGCTCAAGTA
InD2	GGGGCTTATTCACTTTGT	TTCTTTCCTCCTGGCTCT
InD3	CCCTTTTATCCTCAGTAGT	TACAAGCAGTTGTTTGCA
InD4	GGCGACCGAGAAGTGTAG	CGAGATAGCGAAAGCGAC
InD5	CGGGTCAGTGTTATCGTC	CATCAATGAAGGGCAAAT

分子标记 Marker	前引物 (5’-3’) Forward sequence (5’-3’)	后引物 (5’-3’) Reverse sequence (5’-3’)
S1	TAATTGCGGTCTCGTGCC	AACCACCTCAACTACTGCCG
S2	CGCCTGCAAAAAAGGTAGAG	GATCAAAGGAACCCCCGTAG
S3	GCCGGTAGATGCAGTATTGC	GTGCACAGTCACGCAGAAAG
S4	TGCTGTCTCCACTTCCCTTC	AAGGAGGCGTGCTTAACAGG
S5	CTTGTCTTGGCTTGGCTAGG	ATCCACGCAGATAGACCTGC
InD1	AAAACGATCATAGTTGCTTGC	GGAGTTCGGGCTCAAGTA
InD2	GGGGCTTATTCACTTTGT	TTCTTTCCTCCTGGCTCT
InD3	CCCTTTTATCCTCAGTAGT	TACAAGCAGTTGTTTGCA
InD4	GGCGACCGAGAAGTGTAG	CGAGATAGCGAAAGCGAC
InD5	CGGGTCAGTGTTATCGTC	CATCAATGAAGGGCAAAT

基因	功能注释
Gene	Function annotation
LOC_Os05g48700	赤霉素2-β-双加氧酶 Gibberellin 2-beta-dioxygenase
LOC_Os05g48710	表达蛋白 Expressed protein 转座子蛋白Transposon protein
LOC_Os05g48720	转座子蛋白 Transposon protein
LOC_Os05g48730	表达蛋白 Expressed protein
LOC_Os05g48740	表达蛋白 Expressed protein
LOC_Os05g48750	3-脱氧-甘露醇辛酸胞苷酰转移酶 3-deoxy-manno-octulosonate cytidylyltransferase
LOC_Os05g48760	含有DUF1421结构域的功能未知蛋白质 Protein of unknown function containing DUF1421 domain
LOC_Os05g48770	表达蛋白 Expressed protein

基因	功能注释
Gene	Function annotation
LOC_Os05g48700	赤霉素2-β-双加氧酶 Gibberellin 2-beta-dioxygenase
LOC_Os05g48710	表达蛋白 Expressed protein 转座子蛋白Transposon protein
LOC_Os05g48720	转座子蛋白 Transposon protein
LOC_Os05g48730	表达蛋白 Expressed protein
LOC_Os05g48740	表达蛋白 Expressed protein
LOC_Os05g48750	3-脱氧-甘露醇辛酸胞苷酰转移酶 3-deoxy-manno-octulosonate cytidylyltransferase
LOC_Os05g48760	含有DUF1421结构域的功能未知蛋白质 Protein of unknown function containing DUF1421 domain
LOC_Os05g48770	表达蛋白 Expressed protein