Identification and Gene Mapping of a New Photo-thermo-sensitive Male Sterile Mutant tms3650 in Rice

doi:10.16819/j.1001-7216.2023.220109

Abstract

Abstract:

【Objective】 Male sterility is the basis of rice heterosis utilization. To further analyze the regulation mechanism, a male sterility mutant was identified.【Method】 The tms3650 mutant was isolated from the mutant library of ⁶⁰Co-γ-treated indica cultivar 93-11. The indica rice Minghui 63 was crossed with the mutant tms3650 as the male parent to construct F₂ and F₃ populations, and the target gene was fine-mapped by map-based cloning. 【Result】 The other agronomic traits of tms3650 were the same as those of the wild type, but the anthers of the mutant were white-green and thin, the pollen could not be stained blue-black by 1% iodine-potassium iodide solution with panicle-enclosure and delayed heading stage. Genetic analysis indicated that the mutant phenotype was controlled by a pair of recessive nuclear genes. The results of fine mapping showed that the gene was located within a distance of 135.25 kb between the SSR markers RM15927 and RM15934 on the long arm of chromosome 3, and co-segregated with the RM15931 marker. The identification of southern breeding in Lingshui in winter revealed that the fertility of mutant was affected by light and temperature, indicating that the tms3650 was a photo-thermo-sensitive male sterile mutant. 【Conclusion】 By comparing with the reported male sterility genes, the tms3650 was controlled by a new gene locus, TMS3650.

Key words: rice, photo-thermo-sensitive male sterility, gene mapping

摘要： 目的雄性不育是水稻杂种优势利用的基础。为进一步解析其调控机制，对一个雄性不育突变体进行了鉴定。方法利用⁶⁰Co-γ对籼稻品种93-11进行辐射诱变，从其后代中鉴定到一个雄性不育突变体tms3650。将籼稻明恢63作父本同突变体tms3650杂交构建F₂和F₃群体，采用图位克隆的方法对目的基因进行精细定位。结果 tms3650突变体其他农艺性状与野生型一致，但花药白绿且瘦小，花粉不能被1%碘-碘化钾溶液染成蓝黑色，穗子包颈，抽穗期延迟。遗传分析表明该突变体表型受一对隐性核基因控制。精细定位结果表明基因位于第3染色体长臂SSR标记RM15927和RM15934之间135.25 kb距离内，且与RM15931标记共分离。陵水冬季南繁鉴定发现，该突变体育性受光温环境影响，说明tms3650突变体的雄性育性在短日低温条件下发生了转育，是一个光温敏雄性不育突变体。结论通过将定位位点与已报道的雄性不育基因比较，发现tms3650是一个新的基因位点，暂命名为TMS3650。

关键词: 水稻, 光温敏雄性不育, 基因定位

TANG Jie, LONG Tuan, WU Chunyu, LI Xinpeng, ZENG Xiang, WU Yongzhong, HUANG Peijin. Identification and Gene Mapping of a New Photo-thermo-sensitive Male Sterile Mutant tms3650 in Rice[J]. Chinese Journal OF Rice Science, 2023, 37(1): 45-54.

唐杰, 龙湍, 吴春瑜, 李新鹏, 曾翔, 吴永忠, 黄培劲. 水稻光温敏雄性不育突变体tms3650的鉴定和基因定位[J]. 中国水稻科学, 2023, 37(1): 45-54.

Figures/Tables 8

References 59

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育性 Fertility	株系Line									合计 Total
育性 Fertility	L1	L2	L3	L4	L5	L6	L7	L8	L9	合计 Total
可育花粉数Fertile pollen	170	174	156	120	202	71	95	100	39	1127
不育花粉数Sterile pollen	195	215	271	130	190	85	104	81	16	1287
自交收种Selfed seed	240	200	300	320	380	117	194	220	90	2061
结实率Seed setting rate/%	32.0	26.6	40.0	42.6	50.6	15.6	25.8	29.3	12.0	30.5

育性 Fertility	株系Line									合计 Total
育性 Fertility	L1	L2	L3	L4	L5	L6	L7	L8	L9	合计 Total
可育花粉数Fertile pollen	170	174	156	120	202	71	95	100	39	1127
不育花粉数Sterile pollen	195	215	271	130	190	85	104	81	16	1287
自交收种Selfed seed	240	200	300	320	380	117	194	220	90	2061
结实率Seed setting rate/%	32.0	26.6	40.0	42.6	50.6	15.6	25.8	29.3	12.0	30.5

杂交组合 Cross combination	F₁结实率 Seed-setting rate of F₁/%	F₂		χ²_3:1	χ²_0.05
杂交组合 Cross combination	F₁结实率 Seed-setting rate of F₁/%	野生型 Wild type plant	突变体 Mutant plant	χ²_3:1	χ²_0.05
tms3650/93-11	58.13	79	31	0.44	3.841
tms3650/ZH11	76.92	56	14	0.70	3.841
tms3650/明恢63 tms3650/Minghui 63	75.19	186	61	0.01	3.841

杂交组合 Cross combination	F₁结实率 Seed-setting rate of F₁/%	F₂		χ²_3:1	χ²_0.05
杂交组合 Cross combination	F₁结实率 Seed-setting rate of F₁/%	野生型 Wild type plant	突变体 Mutant plant	χ²_3:1	χ²_0.05
tms3650/93-11	58.13	79	31	0.44	3.841
tms3650/ZH11	76.92	56	14	0.70	3.841
tms3650/明恢63 tms3650/Minghui 63	75.19	186	61	0.01	3.841

序号 Serial number	基因 Gene	功能注释 Function annotation
1	LOC_Os03g53570	反转录转座子蛋白 Retrotransposon protein, putative, unclassified, expressed
2	LOC_Os03g53580	表达蛋白 Expressed protein
3	LOC_Os03g53590	表达蛋白 Expressed protein
4	LOC_Os03g53600	HTH DNA结合蛋白 HTH DNA-binding protein, putative, expressed
5	LOC_Os03g53610	晚期胚胎发育丰富的蛋白质 D-34 late embryogenesis abundant protein D-34, putative, expressed
6	LOC_Os03g53620	晚期胚胎发育丰富的蛋白质 D-34 late embryogenesis abundant protein D-34, putative, expressed
7	LOC_Os03g53630	PHD锌指家族蛋白 PHD finger family protein, putative, expressed
8	LOC_Os03g53640	表达蛋白 Expressed protein
9	LOC_Os03g53650	半胱氨酸合酶 Cysteine synthase, putative, expressed
10	LOC_Os03g53660	含有肌球蛋白头部结构域的蛋白质 Myosin head domain containing protein, expressed
11	LOC_Os03g53670	含有YT521-B样家族结构域的蛋白质 YT521-B-like family domain containing protein, expressed
12	LOC_Os03g53680	表达蛋白 Expressed protein
13	LOC_Os03g53690	氧化还原酶，含有短链脱氢酶/还原酶家族结构域的蛋白质 Oxidoreductase, short chain dehydrogenase/reductase family domain containing protein, expressed
14	LOC_Os03g53700	PHD锌指家族蛋白 PHD-finger domain containing protein, putative, expressed
15	LOC_Os03g53710	醛糖1-差向异构酶 Aldose 1-epimerase, putative, expressed
16	LOC_Os03g53720	SRPK4蛋白 SRPK4, putative, expressed
17	LOC_Os03g53730	黄素蛋白wrbA Flavoprotein wrbA, putative, expressed
18	LOC_Os03g53740	表达蛋白 Expressed protein
19	LOC_Os03g53750	核前蛋白A识别因子 Nuclear prelamin A recognition factor, putative, expressed
20	LOC_Os03g53760	核前蛋白A识别因子 Nuclear prelamin A recognition factor, putative, expressed
21	LOC_Os03g53770	含有蛋白质的RNA识别基序 RNA recognition motif containing protein, putative, expressed
22	LOC_Os03g53780	铵转运体2 Ammonium transporter 2, putative, expressed
23	LOC_Os03g53790	周质β-葡萄糖苷酶前体 Periplasmic beta-glucosidase precursor, putative, expressed
24	LOC_Os03g53800	周质β-葡萄糖苷酶前体 Periplasmic beta-glucosidase precursor, putative, expressed