Effect of Feed Protein Contents on Gaseous Nitrogen Emissions, Growth Performance of Catfish and Nitrogen Utilization in Rice-yellow Catfish Co-culture Model

doi:10.16819/j.1001-7216.2022.211008

Abstract

Abstract:

【Objective】 Clarifying the effect of protein contents in the feeds on N₂O and NH₃ emissions and on the growth of yellow catfish (Pelteobagrus fulvidraco) is conducive to the efficient and green development of rice-catfish co-culture model. 【Methods】In this study, pot-experiments were conducted to systematically study the effects of different dietary protein contents on the dynamics of N₂O and NH₃ flux rates, the nitrogen accumulation in the water and bottom soil, and the growth performance of yellow catfish in the rice-catfish co-culture treatments compared to the catfish monoculture. 【Results】 Rice-catfish co-culture significantly reduced the N₂O emission, NH₃ emission, total and inorganic nitrogen in the water by 18.30%, 76.20%, 41.30% and 48.85%, and significantly increased the protein accumulation of catfish and nitrogen use efficiency by 20.00% and 171.50%, respectively, as compared with catfish monoculture under the same protein streatment. The N₂O emission and residual nitrogen in the water of rice-catfish co-culture treatments increased with the rise of the contents of protein in the feeds, but the NH₃ flux rates did not show positive correlation with the contents of protein in the feeds. The specific growth rate and protein gain of catfish showed a quadratic correlation with the contents of protein in the feeds. And the nitrogen use efficiency decreased linearly with the increase of protein contents in the feeds.【Conclusion】The recommended protein contents in the feeds for rice-catfish co-culture model is less than 43.04% based on the synthetical evaluation on the effect of protein contents on the growth performance of catfish, N₂O emission, and residue of nitrogen in the aquaculture water.

Key words: rice-yellow catfish co-culture, feed protein content, N₂O emission, NH₃ volatilization, growth

摘要：

【目的】明确饲料蛋白含量对稻-黄颡鱼共作模式下N₂O和NH₃挥发特征及黄颡鱼生长的影响，有利于稻-黄颡鱼综合种养的高效、绿色发展。【方法】采用盆栽模拟试验，以常规单养黄颡鱼模式为对照，系统研究了不同饲料蛋白含量对稻-黄颡鱼共作模式下N₂O和NH₃的排放特征、水体和底泥的氮含量以及黄颡鱼生长的影响。【结果】在相同蛋白含量下，稻-黄颡鱼共作模式的N₂O和NH₃挥发分别比单养黄颡鱼模式降低18.3%和76.20%，水体总氮和无机氮含量降低41.30%和48.85%，黄颡鱼蛋白累积量增加20.00%，氮利用率增加171.50%。在稻-黄颡鱼共作模式下，提高饲料蛋白含量会显著增加N₂O排放量和水体残留氮含量，但对NH₃挥发无显著影响;黄颡鱼特定生长率和蛋白累积量与饲料蛋白含量呈二次曲线关系。饲料氮利用效率随饲料蛋白含量增加呈线性降低趋势。【结论】综合考虑黄颡鱼生长和N₂O排放以及养殖水体氮残留等因素，确定稻-黄颡鱼共作模式饲料蛋白含量不宜超过43.04%。

关键词: 稻-黄颡鱼共作, 饲料蛋白含量, N₂O排放, NH₃挥发, 生长

WANG Mengjie, WU Junnan, LIU Yaobin, XU Chunchun, FENG Jinfei, LI Fengbo, FANG Fuping. Effect of Feed Protein Contents on Gaseous Nitrogen Emissions, Growth Performance of Catfish and Nitrogen Utilization in Rice-yellow Catfish Co-culture Model[J]. Chinese Journal OF Rice Science, 2022, 36(4): 428-438.

王梦杰, 吴俊男, 刘耀斌, 徐春春, 冯金飞, 李凤博, 方福平. 饲料蛋白含量对稻-黄颡鱼共作模式气态氮排放、黄颡鱼生长和氮利用率的影响[J]. 中国水稻科学, 2022, 36(4): 428-438.

Figures/Tables 10

References 32

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原料成分 Raw material	配方目标含量 Formula target content
原料成分 Raw material	45%	40%	35%	30%	25%
豆粕Soybean meal/g	24.00	25.00	22.00	18.00	24.00
鱼粉Fish meal/g	49.00	39.00	31.00	24.00	12.00
小麦粉Wheat flour/g	9.00	14.00	17.00	27.00	38.00
麦麸 Wheat bran/g	10.00	14.00	21.00	22.00	17.00
微晶纤维素Microcrystallie cellulose/g	1.50	1.50	1.50	1.50	1.50
菜籽油 Rapeseed oil/g	1.00	1.00	2.00	2.00	2.00
卵磷脂Lecthin/g	4.00	4.00	4.00	4.00	4.00
抗氧化剂Antioxidants/g	1.50	1.50	1.50	1.50	1.50
总计Total/g	100.00	100.00	100.00	100.00	100.00
粗蛋白含量Crude protein content/%	45.16	39.38	34.15	31.52	26.73
粗脂肪含量Crude fat content/%	9.75	9.66	9.75	9.75	8.79
总能量Total energy/(kJ·g⁻¹)	22.37	20.51	19.12	18.17	15.80

原料成分 Raw material	配方目标含量 Formula target content
原料成分 Raw material	45%	40%	35%	30%	25%
豆粕Soybean meal/g	24.00	25.00	22.00	18.00	24.00
鱼粉Fish meal/g	49.00	39.00	31.00	24.00	12.00
小麦粉Wheat flour/g	9.00	14.00	17.00	27.00	38.00
麦麸 Wheat bran/g	10.00	14.00	21.00	22.00	17.00
微晶纤维素Microcrystallie cellulose/g	1.50	1.50	1.50	1.50	1.50
菜籽油 Rapeseed oil/g	1.00	1.00	2.00	2.00	2.00
卵磷脂Lecthin/g	4.00	4.00	4.00	4.00	4.00
抗氧化剂Antioxidants/g	1.50	1.50	1.50	1.50	1.50
总计Total/g	100.00	100.00	100.00	100.00	100.00
粗蛋白含量Crude protein content/%	45.16	39.38	34.15	31.52	26.73
粗脂肪含量Crude fat content/%	9.75	9.66	9.75	9.75	8.79
总能量Total energy/(kJ·g⁻¹)	22.37	20.51	19.12	18.17	15.80

处理 Treatment	TN /(mg∙L⁻¹)	NH₄⁺-N /(mg∙L⁻¹)	NO₃⁻-N /(mg∙L⁻¹)	NO₂⁻-N /(mg∙L⁻¹)
RF-P25	4.31±0.22 e	0.58±0.07 c	1.91±0.15 e	0.17±0.02 c
RF-P30	5.67±0.15 d	2.04±0.51 b	2.36±0.17 d	0.21±0.03 c
RF-P35	6.08±0.48 cd	2.53±0.56 b	2.72±0.08 c	0.28±0.03 b
RF-P40	6.61±0.1 c	2.64±0.26 b	3.27±0.09 b	0.30±0.02 b
RF-P45	7.24±0.33 b	2.87±0.22 b	3.47±0.12 b	0.31±0.02 b
F-P40	11.26±0.54 a	5.95±1.16 a	3.68±0.03 a	0.56±0.07 a
F值F value	142.09**	27.70**	106.15**	49.72**

处理 Treatment	TN /(mg∙L⁻¹)	NH₄⁺-N /(mg∙L⁻¹)	NO₃⁻-N /(mg∙L⁻¹)	NO₂⁻-N /(mg∙L⁻¹)
RF-P25	4.31±0.22 e	0.58±0.07 c	1.91±0.15 e	0.17±0.02 c
RF-P30	5.67±0.15 d	2.04±0.51 b	2.36±0.17 d	0.21±0.03 c
RF-P35	6.08±0.48 cd	2.53±0.56 b	2.72±0.08 c	0.28±0.03 b
RF-P40	6.61±0.1 c	2.64±0.26 b	3.27±0.09 b	0.30±0.02 b
RF-P45	7.24±0.33 b	2.87±0.22 b	3.47±0.12 b	0.31±0.02 b
F-P40	11.26±0.54 a	5.95±1.16 a	3.68±0.03 a	0.56±0.07 a
F值F value	142.09**	27.70**	106.15**	49.72**

处理 Treatment	TN /(g∙kg⁻¹)	NH₄⁺-N /(mg∙kg⁻¹)	NO₃⁻-N /(mg∙kg⁻¹)
RF-P25	1.30±0.01 c	25.69±0.81 c	4.42±0.32 a
RF-P30	1.32±0.01 bc	27.27±1.75 bc	4.12±0.17 ab
RF-P35	1.30±0.01 c	29.22±0.76 b	4.00±0.09 b
RF-P40	1.31±0.02 bc	29.18±0.64 b	4.39±0.04 a
RF-P45	1.35±0.01 a	28.66±1.14 b	4.41±0.18 a
F-P40	1.33±0.01 ab	45.27±0.87 a	3.40±0.10 c
F值F value	7.00**	137.156**	15.334**