Relationship Between Canopy Microclimate at Grain Filling Stage and Rice Quality of Directly Seeded Rice Under Water and Nitrogen Interaction

doi:10.16819/j.1001-7216.2021.0314

Abstract

Abstract:

【Objective】Field canopy microclimate is the most direct environment factor for rice growth and development. The management of water and nitrogen fertilizer affects the growth of rice and thus affect the canopy microclimate of paddy field. Studying the effect of water and nitrogen interaction on the canopy microclimate during the seed-filling stage and rice quality of directly seeded rice can enrich and improve water and nitrogen management of the directly seeded rice. 【Method】With hybrid rice F You 498 as material, three irrigation treatments were designed including flooding (W₁), dry-wet alternate (W₂) and drying (W₃) as well as three N fertilizer application methods with basal fertilizer: fertilizer for tillering: topdressing for panicle initiation 5:3:2(N₁), 3:3:4(N₂) and 3:1:6(N₃), The effect of water and nitrogen interaction on canopy temperature, light intensity and rice quality of directly-seeded rice were analyzed during grain filling stage. The relationship between canopy microclimate and rice quality was also discussed. 【Result】The results showed that irrigation mode and N fertilization interactions had a significant or extremely significant effect on milling quality, appearance quality, and cooking and palatability of directly seeded rice. Under the conditions of 150 kg/hm²N application rate for each irrigation method, the most suitable panicle N-fertilizer level should account for 20% of total N. Coupled with W₂, it can further improve rice quality. The ratio of N-fertilizer for panicle initiation increased to 60%, leading to the decrease of daily temperature difference, total temperature difference, accumulated temperature, effective accumulated temperature, daily average light intensity and rice quality of directly seeded rice under irrigation. Under the interaction of water and N, the canopy microclimate in the field exerted the greatest impact on milling quality, and cooking and palatability of directly seeded rice. The effect of temperature on the head milled rice rate was greater than the light intensity, and the effective accumulated temperature had the highest correlation with head milled rice rate. Light intensity was the most closely related to the appearance, taste and comprehensive score of rice cooking and palatability quality. 【Conclusion】It is preliminarily proved that the interaction between water and nitrogen affects rice quality by changing the microclimate of the direct-seeded rice canopy. The results will lay a theoretical and practical basis for improvement of the quality and yield of direct-seeded rice.

Key words: directly seeded rice, water management mode, N fertilizer, canopy microclimate, rice quality

摘要：

【目的】结实期田间穗部冠层小气候是水稻生长发育最直接的微环境因子,水分和氮肥运筹通过影响水稻群体构建进而影响水稻田间冠层小气候,研究水氮互作对直播稻结实期冠层小气候及稻米品质的影响,可以丰富和完善直播稻水氮管理技术。【方法】以杂交稻F优498为试材,通过淹灌(W₁)、干湿交替(W₂)、旱种(W₃)3种灌水方式及底肥:蘖肥:穗肥质量比分别为5:3:2(N₁)、3:3:4(N₂)、3:1:6(N₃)3种氮肥运筹模式,研究水氮互作对直播稻灌浆结实期冠层温度、光照强度及稻米品质的影响;并探讨水氮互作下直播稻灌浆结实期穗部冠层小气候特征与米质的关系。【结果】水氮互作对稻米加工、外观及食味品质均存在显著或极显著的影响。各灌水方式在150 kg/hm²施氮量下,氮肥后移比例均以占总施氮量的20%最优,且与W₂处理耦合可进一步改善稻米品质。各灌溉方式下,氮肥后移比例增加到总量的60%时,会导致直播稻灌浆结实期温差、总温差、积温、有效积温、光照强度及米质下降。水氮互作下穗部冠层小气候与加工品质及蒸煮食味品质关系密切,温度对整精米率的影响大于光照强度,且有效积温与整精米率相关性最高;光照强度与稻米蒸煮食味品质的外观、口感以及综合评分关系最密切【结论】初步探明,水氮互作会通过改变直播稻冠层小气候进而影响稻米品质。可为直播稻提质丰产增效提供理论依据与实践基础。

关键词: 直播稻, 灌溉方式, 氮肥运筹, 冠层小气候, 米质

Yunxia WU, Changchun GUO, Yongjian SUN, Fangyan LIU, Zhiyuan YANG, Yuanyuan SUN, Mingtian MA, Jun MA. Relationship Between Canopy Microclimate at Grain Filling Stage and Rice Quality of Directly Seeded Rice Under Water and Nitrogen Interaction[J]. Chinese Journal OF Rice Science, 2021, 35(3): 269-278.

武云霞, 郭长春, 孙永健, 刘芳艳, 杨志远, 孙园园, 王明田, 马均. 水氮互作下直播稻结实期冠层小气候与米质的关系[J]. 中国水稻科学, 2021, 35(3): 269-278.

Figures/Tables 7

References 25

[1]	李新. 塔里木盆地绿洲边缘农田小气候特征分析[J]. 干旱区地理, 1992(2): 27-32.
	Li X. Analysis of microclimate characteristics of farmland in oasis edge of Tarim Basin[J]. Arid Land Geography, 1992(2): 27-32. (in Chinese)
[2]	李旭毅, 孙永健, 程宏彪, 郑洪帧, 杨志远, 贾现文, 刘树金, 胡蓉, 马均. 氮肥运筹和栽培方式对杂交籼稻Ⅱ优498结实期群体光合特性的影响等.[J]. 作物学报, 2011, 37(9): 1650-1659.
	Li X Y, Sun Y J, Cheng H B, Zheng H Z, Yang Z Y, Jia X W, Liu S J, Hu R, Ma J.Effects of nitrogen application strategy and cultivation model on the performances of canopy apparent photosynthesis of indica hybrid rice Ryou 498 during filling stage[J]. Acta Agronomica Sinica, 2011, 37(9): 1650-1659. (in Chinese with English abstract)
[3]	齐华, 梁熠, 赵明, 王敬亚, 吴亚男, 刘明. 栽培方式对玉米群体结构的调控效应[J]. 华北农学报, 2010, 25(3): 134-13.
	Qi H, Liang Y, Zhao M, wang J Y, Wu Y N, Liu M. The effects of cultivation ways on population structure of maize[J]. Acta Agriculturae Boreali-Sinica, 2011, 37(9): 1650-1659. (in Chinese with English abstract)
[4]	彭小光. 不同施氮条件下水稻农田小气候温湿度变化研究[D]. 长沙: 湖南农业大学, 2007.
	Peng X G.Study on the change of microclimate temperature and humidity in paddy field under different nitrogen application conditions[D]. Changsha: Hunan Agricultural University, 2007. (in Chinese with English abstract)
[5]	He F.Effects of N ratio on canopy microclimate and population health in irrigated rice[J]. Agricultural Science & Technology, 2009, 10(6): 79-83.
[6]	何生兵. 水稻生态节水灌溉模式研究[D]. 南京: 河海大学, 2007.
	He S B.Study on ecological water-saving irrigation model of rice[D]. Nanjing: Hehai University, 2007. (in Chinese with English abstract)
[7]	徐栋, 朱盈, 周磊, 韩超, 郑雷鸣, 张洪程, 魏海燕, 王珏, 廖桉桦, 蔡仕博. 不同类型籼粳杂交稻产量和品质性状差异及其与灌浆结实期气候因素间的相关性[J]. 作物学报, 2018, 44(10): 1548-1559.
	Xu D, Zhu Y, Zhou L, Han C, Zheng L M, Zhang H C, Wei H X, Wang J, Liao A Y, Cai S B.Differences in yield and quality characters of different types of Indica-Japonica hybrid rice and their correlation with climatic factors at grain filling stage[J]. Acta Agronomica Sinica, 2018, 44(10): 1548-1559. (in Chinese with English abstract)
[8]	徐富贤, 周兴兵, 刘茂, 蒋鹏, 张林, 郭晓艺, 朱永川, 熊洪. 川南冬水田杂交中稻品种与气候互作对稻米品质的影响[J]. 中国生态农业学报, 2018, 26(8): 1137-1148.
	Xu F X, Zhou X B, Liu M, Jiang P, Zhao L, Guo X Y, Zhu Y C, Xiong H.Effects of interaction between medium hybrid rice varieties and climate on rice quality in winter paddy field in south Sichuan[J]. Chinese Journal of Eco-Agriculture, 2018, 26(8): 1137-1148. (in Chinese with English abstract)
[9]	高继平, 张文忠, 隋阳辉, 姚晨, 高明超, 赵明辉. 水分胁迫下水稻拔节孕穗期冠气温度差与产量及品质的关系[J]. 核农学报, 2016, 30(3): 596-604.
	Gao J P, Zhang W Z, Sui Y H, Yao C, Gao M C, Zhao M H.Relationship between canopy temperature difference and yield and quality of rice at jointing and booting stage under water stress[J]. Journal of Nuclear Agricultural Sciences, 2016, 30(3): 596-604. (in Chinese with English abstract)
[10]	韩立宇, 刘洁, 董明辉. 水分和氮肥对大穗型水稻籽粒灌浆结实的影响与生理分析[J]. 中国稻米, 2014, 20(5): 8-12.
	Han L Y, Liu J, Dong M H.Effects of water and nitrogen fertilizer on grain filling and fruiting of large panicle rice and its physiological analysis[J]. China Rice, 2014, 20(5): 8-12. (in Chinese with English abstract)
[11]	武云霞, 刘芳艳, 孙永健, 郭长春, 杨志远, 何艳, 严田蓉, 孙知白, 丁峰, 马均. 水氮互作对直播稻产量及稻米品质的影响[J]. 四川农业大学学报, 2019, 37(5): 604-610, 622.
	Wu Y X, Liu F Y, Sun Y J, Guo C C, Yang Z Y, He Y, Yan T Y, Sun Z B, Ding F, Ma J.Effects of interaction of water and nitrogen on yield and quality of direct seeding rice[J]. Journal of Sichuan Agricultural University, 2019, 37(5): 604-610, 622. (in Chinese with English abstract)
[12]	孙永健, 杨志远, 孙园园, 徐徽, 严奉君, 赵建红, 马均. 成都平原两熟区水氮管理模式与磷钾肥配施对杂交稻冈优725产量及品质的影响[J]. 植物营养与肥料学报, 2014, 20(1): 17-28.
	Sun Y J, Yang Z Y, Sun Y Y, Xu W, Yan F J, Zhao J H, Ma J. Effects of water and nitrogen management model and combined application of phosphorus and potassium fertilizer on yield and quality of hybrid rice Gangyou 725 in double cropping area of Chengdu plain[J]. Journal of Plant Nutrition and Fertilizers,2014, 20(1): 17-28. (in Chinese with English abstract)
[13]	武云霞, 郭长春, 孙永健, 刘芳艳, 张桥, 向开宏, 孙园园, 马均. 水氮互作下直播稻群体质量与氮素利用特征的关系[J]. 应用生态学报, 2020, 31(3): 899-908.
	Wu Y X, Guo C C, Sun Y J, Liu F Y, Zhang Q, Xiang K H, Sun Y Y, Ma J.Relationship between population quality and nitrogen utilization characteristics of direct seeding rice under water-nitrogen interaction[J]. Chinese Journal of Applied Ecology,2020, 31(3): 899-908. (in Chinese with English abstract)
[14]	黎星, 汪勇, 成臣, 程慧煌, 谭雪明, 商庆银, 石庆华, 曾勇军. 氮肥运筹对南方优质常规晚粳产量和品质的影响[J]. 中国稻米, 2019, 25(1): 29-33.
	Li X, Wang Y, Cheng C, Cheng H H, Tan X M, Shang Q Y, Shi Q H, Zeng Y J.Effects of nitrogen fertilizer application on yield and quality of high-quality conventional late japonica rice in south China[J]. China Rice, 2019, 25(1): 29-33. (in Chinese with English abstract)
[15]	周婵婵, 黄元财, 贾宝艳, 王岩, 李瑞峰, 王术, 冯跃, Fugen Dou.施氮量和灌溉方式的交互作用对东北粳稻稻米品质的影响[J]. 中国水稻科学, 2019, 33(4): 357-367.
	Zhou C C, Huang Y C, Jia B Y, Wang Y, Li R F, Wang S, Feng Y, Dou F G.Effects of interaction between nitrogen application rate and irrigation methods on rice quality of japonica rice in northeast China[J]. Chinese Journal of Rice Science, 2019, 33(4): 357-367. (in Chinese with English abstract)
[16]	季红娟, 张小祥, 戴正元, 王宝和, 谭长乐, 张洪熙, 赵步洪. 肥料管理对直播粳稻扬粳4038稻米品质影响的研究[J]. 扬州大学学报: 农业与生命科学, 2011, 32(4): 39-44.
	Ji H J, Zhang X X, Dai Z Y, Wang B H, Tan C L, Zhang H X, Zhao B H.Effect of fertilizer management on rice quality of direct-seeded japonica rice Yangjing 4038[J]. Journal of Yangzhou University: Agricultural and Life Science Edition, 2011, 32(4): 39-44. (in Chinese with English abstract)
[17]	徐正进, 陈温福, 马殿荣, 吴晓冬, 郑煜焱, 王嘉宇. 辽宁水稻食味值及其与品质性状的关系[J]. 作物学报, 2005, 31(8): 1092-1094.
	Xu Z J, Chen W F, Ma D R, Wu X D, Zheng Y Y, Wang J Y.Taste value and its relationship with quality characters of rice in Liaoning Province[J]. Acta Agronomica Sinica, 2005, 31(8): 1092-1094. (in Chinese with English abstract)
[18]	程旺大. 水稻节水高效栽培的生理生态效应及对产量与品质的影响[D]. 杭州: 浙江大学, 2001.
	Cheng W D.Physiological and ecological effects of water-saving and efficient cultivation of rice and its effects on yield and quality[D]. Hangzhou: Zhejiang University, 2001. (in Chinese with English abstract)
[19]	杨国涛, 范永义, 卓驰夫, 黎腊梅, 王萌冉, 彭友林, 胡运高. 氮肥处理对水稻群体小气候及其产量的影响[J]. 云南大学学报: 自然科学版, 2017, 39(2): 324-332.
	Yang G T, Fan Y Y, Zhuo C F, Li L M, Wang M R, Peng Y L, Hu Y G.Effects of nitrogen fertilizer treatment on population microclimate and yield of rice[J]. Journal of Yunnan University: Natural Sciences Edition, 2017, 39(2): 324-332.
[20]	周立宏. 稻田微气候及其对水稻产量和品质的影响[D]. 沈阳: 沈阳农业大学, 2016: 74-80.
	Zhou L H.Microclimate in paddy field and its effect on yield and quality of rice[D]. Shenyang: Shenyang Agricultural University, 2016: 74-80.
[21]	杨东, 段留生, 谢华安, 黄庭旭. 不同生育期弱光对超级稻Ⅱ优航2号产量及品质的影响[J]. 福建农业学报, 2013, 28(2): 107-112.
	Yang D, Duan L S, Xie H A, Huang T X.Effects of low light at different growth stages on yield and quality of super riceⅡYouhang 2[J]. Fujian Journal of Agricultural Sciences, 2013, 28(2): 107-112. (in Chinese with English abstract)
[22]	董明辉, 惠锋, 顾俊荣, 陈培峰, 杨代凤, 乔中英. 灌浆期不同光强对水稻不同粒位籽粒品质的影响[J]. 中国生态农业学报, 2013, 21(2): 164-170.
	Dong M H, Hui F, Gu J R, Chen P F, Yang D F, Qiao Z Y.Effects of different light intensity at grain filling stage on grain quality at different grain positions in rice[J]. Chinese Journal of Eco-Agriculture, 2013, 21(2): 164-170. (in Chinese with English abstract)
[23]	谢光辉, 杨建昌, 王志琴, 朱庆森. 水稻籽粒灌浆特性及其与籽粒生理活性的关系[J]. 作物学报, 2001, 27(5): 557-565.
	Xie G H, Yang J C, Wang Z Q, Zhu Q S.Grain filling characteristics and its relationship with grain physiological activity in rice[J]. Acta Agronomica Sinica, 2001, 27(5): 557-565. (in Chinese with English abstract)
[24]	何海兵, 杨茹, 廖江, 武立权, 孔令聪, 黄义德. 水分和氮肥管理对灌溉水稻优质高产高效调控机制的研究进展[J]. 中国农业科学, 2016, 49(2): 305-318.
	He H B, Yang R, Liao J, Wu L Q, Kong L C, Huang Y D.Research progress on regulation mechanism of water and nitrogen fertilizer management on high quality, high yield and high efficiency of irrigated rice[J]. Scientia Agricultura Sinica, 2016, 49(2): 305-318. (in Chinese with English abstract)
[25]	朱宽宇. 水氮交互作用对水稻弱势粒灌浆的影响[D]. 扬州: 扬州大学, 2016: 34-45.
	Zhu K Y.Effects of interaction of water and nitrogen on grain filling of inferior grains in rice[J]. Yangzhou: Yangzhou University, 2016: 34-45. (in Chinese with English abstract)

处理 Treatment	日均温差 Daily average temperature difference/℃	总温差 Total temperature difference/℃	积温 Accumulated temperature/℃	有效积温 Effective accumulated temperature/℃
W₁N₀	11.08±3.25	400.47±2.82	957.4±3.2	576.8±2.6
W₁N₁	10.42±1.08	402.47±1.93	961.4±1.4	582.4±1.2
W₁N₂	10.33±2.87	400.21±3.27	963.8±1.6	583.6±2.4
W₁N₃	8.27±1.55	336.89±4.87	947.6±2.7	556.3±1.6
W₂N₀	11.30±2.25	403.70±2.24	959.5±2.1	579.5±2.0
W₂N₁	10.83±2.48	411.54±3.26	963.3±2.2	583.3±1.9
W₂N₂	10.59±2.27	402.70±1.00	964.0±2.0	584.0±2.0
W₂N₃	8.94±2.03	339.47±1.64	949.8±1.9	568.8±1.8
W₃N₀	11.20±3.20	403.20±2.25	931.6±1.9	571.6±1.7
W₃N₁	10.75±3.11	408.50±2.56	951.3±1.9	581.3±1.8
W₃N₂	10.57±3.14	401.66±3.12	952.1±1.8	582.1±1.9
W₃N₃	9.42±3.18	339.12±2.64	878.8±1.6	518.8±1.9
W×N	5.02**	2.87*	2.68*	3.72*

处理 Treatment	日均温差 Daily average temperature difference/℃	总温差 Total temperature difference/℃	积温 Accumulated temperature/℃	有效积温 Effective accumulated temperature/℃
W₁N₀	11.08±3.25	400.47±2.82	957.4±3.2	576.8±2.6
W₁N₁	10.42±1.08	402.47±1.93	961.4±1.4	582.4±1.2
W₁N₂	10.33±2.87	400.21±3.27	963.8±1.6	583.6±2.4
W₁N₃	8.27±1.55	336.89±4.87	947.6±2.7	556.3±1.6
W₂N₀	11.30±2.25	403.70±2.24	959.5±2.1	579.5±2.0
W₂N₁	10.83±2.48	411.54±3.26	963.3±2.2	583.3±1.9
W₂N₂	10.59±2.27	402.70±1.00	964.0±2.0	584.0±2.0
W₂N₃	8.94±2.03	339.47±1.64	949.8±1.9	568.8±1.8
W₃N₀	11.20±3.20	403.20±2.25	931.6±1.9	571.6±1.7
W₃N₁	10.75±3.11	408.50±2.56	951.3±1.9	581.3±1.8
W₃N₂	10.57±3.14	401.66±3.12	952.1±1.8	582.1±1.9
W₃N₃	9.42±3.18	339.12±2.64	878.8±1.6	518.8±1.9
W×N	5.02**	2.87*	2.68*	3.72*

灌水方式 Irrigation method	氮肥运筹比例Nitrogen application ratio	糙米率 Brown rice rate /%	整精米率 Head rice rate /%	垩白粒率 Chalky grain rate /%	垩白度 Chalkiness degree /%	长宽比 Aspect ratio
W₁	N₀	80.44 b	62.47 bc	49.60 a	17.93 a	2.60 a
	N₁	81.08 a	63.65 ab	29.67 c	9.67 c	2.53 b
	N₂	81.23 a	62.01 c	33.87 b	12.90 b	2.60 a
	N₃	80.94 a	64.88 a	34.13 b	13.27 b	2.57 ab
	平均Average	80.92	63.25	36.82	13.44	2.57
W₂	N₀	80.26 ab	63.37 a	43.40 a	15.60 a	2.60 a
	N₁	82.05 a	64.18 a	27.63 c	9.60 c	2.53 b
	N₂	80.61 bc	63.05 a	32.40 b	10.63 b	2.57 ab
	N₃	81.17 ab	61.45 a	28.13 c	10.10 bc	2.53 b
	平均Average	81.02	62.49	32.80	11.48	2.56
W₃	N₀	79.31 b	63.05 a	50.37 a	17.37 a	2.53 a
	N₁	80.72 a	63.91 a	38.37 b	13.13 b	2.50 a
	N₂	80.90 a	64.09 a	30.93 c	10.07 c	2.50 a
	N₃	80.57 a	63.18 a	36.47 b	13.87 b	2.53 a
	平均Average	80.38	63.55	39.03	13.61	2.52
F值 F value	W	2.31	0.14	66.60**	12.13*	3.71
	N	43.99**	4.02**	284.03**	169.96**	2.12
	W×N	4.92**	2.72*	12.88**	15.05**	1.24

灌水方式 Irrigation method	氮肥运筹比例Nitrogen application ratio	糙米率 Brown rice rate /%	整精米率 Head rice rate /%	垩白粒率 Chalky grain rate /%	垩白度 Chalkiness degree /%	长宽比 Aspect ratio
W₁	N₀	80.44 b	62.47 bc	49.60 a	17.93 a	2.60 a
	N₁	81.08 a	63.65 ab	29.67 c	9.67 c	2.53 b
	N₂	81.23 a	62.01 c	33.87 b	12.90 b	2.60 a
	N₃	80.94 a	64.88 a	34.13 b	13.27 b	2.57 ab
	平均Average	80.92	63.25	36.82	13.44	2.57
W₂	N₀	80.26 ab	63.37 a	43.40 a	15.60 a	2.60 a
	N₁	82.05 a	64.18 a	27.63 c	9.60 c	2.53 b
	N₂	80.61 bc	63.05 a	32.40 b	10.63 b	2.57 ab
	N₃	81.17 ab	61.45 a	28.13 c	10.10 bc	2.53 b
	平均Average	81.02	62.49	32.80	11.48	2.56
W₃	N₀	79.31 b	63.05 a	50.37 a	17.37 a	2.53 a
	N₁	80.72 a	63.91 a	38.37 b	13.13 b	2.50 a
	N₂	80.90 a	64.09 a	30.93 c	10.07 c	2.50 a
	N₃	80.57 a	63.18 a	36.47 b	13.87 b	2.53 a
	平均Average	80.38	63.55	39.03	13.61	2.52
F值 F value	W	2.31	0.14	66.60**	12.13*	3.71
	N	43.99**	4.02**	284.03**	169.96**	2.12
	W×N	4.92**	2.72*	12.88**	15.05**	1.24

灌水方式Irrigation method	氮肥运筹比例 Nitrogen application ratio	外观 Appearance	口感 Taste	综合评分 Comprehensive score	硬度 Hardness	黏度 Viscosity	平衡 Balance	弹性 Elasticity
W₁	N₀	8.89 a	8.59 a	88.50 a	7.57 a	0.21 d	0.030 d	0.750 a
	N₁	8.70 ab	8.47 a	86.67 a	6.16 b	0.27 c	0.043 c	0.713 b
	N₂	8.53 b	8.17 b	80.00 b	7.00 a	0.51 a	0.073 a	0.735 a
	N₃	7.87 c	7.40 c	77.67 c	7.45 a	0.43 b	0.057 b	0.703 b
	平均Average	8.50	8.15	83.21	7.05	0.35	0.051	0.725
W₂	N₀	8.67 a	8.68 a	87.67 a	6.40 a	0.37 a	0.060 a	0.685 b
	N₁	8.67 a	8.43 a	86.33 a	6.58 a	0.30 b	0.047 b	0.755 a
	N₂	7.95 b	7.90 b	79.67 b	7.20 a	0.37 a	0.060 a	0.750 a
	N₃	7.90 b	7.63 bc	78.00 b	6.94 a	0.33 ab	0.050 ab	0.733 a
	平均Average	8.30	8.16	82.92	6.78	0.34	0.054	0.731
W₃	N₀	8.73 a	8.53 a	87.50 a	6.56 ab	0.37 ab	0.057 a	0.710 bc
	N₁	8.05 b	7.93 b	83.33 b	6.29 b	0.32 b	0.055 a	0.745 a
	N₂	7.87 b	7.63 bc	78.00 c	7.60 a	0.41 a	0.055 a	0.740 ab
	N₃	7.80 b	7.53 c	77.67 c	6.59 ab	0.39 a	0.060 a	0.690 c
	平均Average	8.11	7.91	81.63	6.76	0.37	0.057	0.721
F值 F value	W	8.79*	1.98	3.65	1.69	0.93	1.09	1.29
	N	30.36**	55.01**	61.01**	2.84	22.73**	5.79**	5.75**
	W×N	3.17*	2.70*	3.71*	1.33	10.43**	5.44**	4.82**