QTL Analysis for Heading Date in Rice (Oryza sativa L.) Under Different Temperatures and Light Intensities

doi:10.16819/j.1001-7216.2016.5172

Abstract

Abstract:

QTLs for heading date were identified with 85 rice chromosome single segment substitution lines (CSSSLs) derived from Nipponbare as donor parent in the background of Guanglu’ai 4, for means comparisons between CSSSLs and the recipient parent Guanglu’ai 4 by one-way analysis of variance and Dunnett’s test. A total of 40 QTLs for heading date were identified on all 12 chromosomes under two different temperatures and light intensities with a significance of P≤0.001. Twenty-one QTLs for heading date were mapped in the two environments, 15 QTLs only in Nanjing, and four only in Sanya. Thirty-six QTLs were mapped in Nanjing, the additive effects of which ranged from 2.8 d to 15.7 d, the additive effect percentages from 3.8% to 21.1%; 25 QTLs were mapped in Sanya, the additive effect of which ranged from 1.8 to 12.1 d, the additive effect percentages from 1.7% to 11.3%. The results are important for the QTLs cloning and breeding of new high-quality rice variety in different environments.

Key words: rice, chromosome single segment substitution lines, quantitative trait loci, heading date, substitution mapping

摘要：

以广陆矮4号为受体,日本晴为供体的85个染色体单片段代换系群体为试验材料,通过单因素方差分析和Dunnett多重比较,测验单片段代换系与广陆矮4号之间抽穗期的差异,对代换片段上抽穗期相关的QTL进行了定位。以P≤0.001为阈值,在南京和海南不同温光条件下共定位到40个抽穗期相关的QTL。其中,21个QTL在2个环境中均被检测到;15个QTL只在南京环境中被检测到;4个QTL只在海南环境中被检测到。南京环境中定位到的36个抽穗期相关QTL,其加性效应值变化范围为2.8 d~15.7 d,加性效应百分率变化范围为3.8%~21.1%;海南环境中定位到的25个抽穗期相关QTL,加性效应值变化范围为1.8 d~12.1 d,加性效应百分率变化范围为1.7%~11.3%。这些QTL的定位,为进一步精细定位并克隆相应主效QTL和优异品种特定环境下的生育期改良奠定了基础。

关键词: 水稻, 染色体单片段代换系, 数量性状基因座, 抽穗期, 代换作图

CLC Number:

Jun WANG, Jin-yan ZHU, Yong ZHOU, Jie YANG, Fang-jun FAN, Wen-qi LI, Fang-quan WANG, Wei-gong ZHONG, Guo-hua LIANG. QTL Analysis for Heading Date in Rice (Oryza sativa L.) Under Different Temperatures and Light Intensities[J]. Chinese Journal OF Rice Science, 2016, 30(3): 247-255.

王军, 朱金燕, 周勇, 杨杰, 范方军, 李文奇, 王芳权, 仲维功, 梁国华. 不同温光条件下水稻抽穗期QTL的定位与分析[J]. 中国水稻科学, 2016, 30(3): 247-255.

Figures/Tables 4

References 37

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代换系 CSSSLs	代换区间 Substituted segment	长度 Length /Mb	染色体 Chromosome	QTL	E1			E2			类型 Type
代换系 CSSSLs	代换区间 Substituted segment	长度 Length /Mb	染色体 Chromosome	QTL	表型 Phenotype		加型效应 Additive effect/d		加性效应百分率 Additive effect contribution/%	表型 Phenotype	加型效应 Additive effect/d	加性效应百分率 Additive effect contribution/%
C32007	RM6324-S1-3	7.34	1	qHD1.1	67.0	-4.6	-6.0	84.6	-10.3	-9.8	Ⅰ
C32009	Lsts1-34-RM11189	7.92	1	qHD1.2	68.3	-4.0	-5.2	85.4	-9.9	-9.4	Ⅰ
C32016	RM11762-S1-15	0.69	1	qHD1.3	66.0	-5.1	-6.7	82.4	-11.4	-10.8	Ⅰ
C32042	RM13034-RM13263	8.67	2	qHD2.2	70.0	-3.3	-4.3	85.8	-9.9	-9.4	Ⅰ
C32050	S2-30-S2-9	1.01	2	qHD2.3	69.3	-3.7	-4.8	84.8	-10.4	-9.8	Ⅰ
C32076	S4-2	2.50	4	qHD4.1	68.2	-2.8	-3.8	85.8	-8.5	-8.3	Ⅰ
C32121	RM21767-S7-17	5.83	7	qHD7.2	69.2	-4.4	-5.8	85.2	-10.9	-10.2	Ⅰ
C32122	S7-5	1.48	7	qHD7.3	66.0	-6.0	-7.9	82.8	-12.1	-11.3	Ⅰ
C32129	RM22722	1.39	8	qHD8.2	70.4	-3.8	-5.0	86.6	-10.2	-9.5	Ⅰ
C32145	RM25213-S10-24	4.44	10	qHD10.1	67.4	-4.4	-5.8	84.8	-10.2	-9.7	Ⅰ
C32146	S10-27-RM25486	3.62	10	qHD10.2	68.2	-4.0	-5.2	84.2	-10.5	-10.0	Ⅰ
C32153	S11-9-ZHsts11-43	7.15	11	qHD11.3	67.4	-4.4	-5.8	84.6	-10.3	-9.8	Ⅰ
C32163	S12-17	0.80	12	qHD12.2	69.6	-3.3	-4.3	86.6	-9.3	-8.8	Ⅰ
C32070	S3-9-S3-10	3.80	3.0	qHD3.3	99.3	12.8	17.3	109.0	3.1	3	Ⅱ
C32102	RM7158-S6-18	1.50	6	qHD6.1	94.2	10.0	13.5	112.2	4.5	4.4	Ⅱ
C32107	RM20069	0.77	6	qHD6.3	95.2	10.5	14.2	117.2	7.0	6.8	Ⅱ
C32034	Zsts2-1-RM12705	3.81	2	qHD2.1	96.4	9.9	12.9	99.6	-3.0	-2.8	Ⅲ
C32086	RM17305-S4-29	3.79	4	qHD4.2	92.9	9.6	12.9	99.2	-1.8	-1.8	Ⅲ
C32113	RM20659-S6-40	2.24	6	qHD6.5	85.3	5.6	7.5	98.8	-2.2	-2.1	Ⅲ
C32124	S7-26-RM22185	0.33	7	qHD7.4	101.7	11.9	15.6	103.4	-1.8	-1.7	Ⅲ
C32126	RM6925-S8-1	2.55	8	qHD8.1	99.0	10.5	13.9	103.2	-1.9	-1.8	Ⅲ
C32020	S1-15-S1-27	3.60	1	qHD1.4	102.1	13.0	17.0	104.2	-	-	Ⅳ
C32053	RM3894	1.02	2	qHD3.1	92.1	7.8	10.1	103.2	-	-	Ⅳ
C32071	S3-11-S3-12	5.22	3	qHD3.4	90.8	8.5	11.5	101.6	-	-	Ⅳ
C32098	S5-15-RM3170	4.26	5	qHD5.2	100.4	13.1	17.7	102.8	-	-	Ⅳ
C32105	S6-21-S6-7	3.06	6	qHD6.2	88.9	7.4	9.9	104.8	-	-	Ⅳ
C32112	RM162-S6-16	3.08	6	qHD6.4	102.2	14.0	18.9	105.4	-	-	Ⅳ
C32115	RM21344-S7-3	6.60	7	qHD7.1	95.0	10.4	14.0	103.2	-	-	Ⅳ
C32130	RM22825-RM22905	4.32	8	qHD8.3	91.7	6.9	9.0	109.2	-	-	Ⅳ
C32133	RM23175-RM23642	8.07	8	qHD8.4	85.5	3.8	4.9	109.0	-	-	Ⅳ
C32140	S9-4	1.54	9	qHD9.1	97.3	9.7	12.7	109.8	-	-	Ⅴ
C32144	RM24805	1.47	9	qHD9.2	99.4	10.7	14.1	109.0	-	-	Ⅳ
C32147	RM26076-S11-4	2.17	11	qHD11.1	86.8	5.3	7.0	105.8	-	-	Ⅳ
C32154	S11-29-S11-31	4.33	11	qHD11.4	88.7	6.3	8.2	104.8	-	-	Ⅳ
C32157	RM6094	0.08	11	qHD11.5	94.3	9.1	11.9	103.2	-	-	Ⅳ
C32158	RM27460-S12-8	0.56	12	qHD12.1	97.1	10.5	13.7	108.2	-	-	Ⅳ
C32061	RM3434-Zsts3-8	7.20	3	qHD3.2	72.3	-	-	87.2	-7.8	-7.6	Ⅴ
C32088	M17605-RM17693	2.51	4	qHD4.3	70.0	-	-	86.4	-8.4	-8.1	Ⅴ
C32091	S5-1	1.70	5	qHD5.1	70.2	-	-	86.6	-8.3	-8.0	Ⅴ
C32149	S11-5-RM26343	3.60	11	qHD11.2	72.4	-	-	84.8	-10.2	-9.7	Ⅴ

代换系 CSSSLs	代换区间 Substituted segment	长度 Length /Mb	染色体 Chromosome	QTL	E1			E2			类型 Type
代换系 CSSSLs	代换区间 Substituted segment	长度 Length /Mb	染色体 Chromosome	QTL	表型 Phenotype		加型效应 Additive effect/d		加性效应百分率 Additive effect contribution/%	表型 Phenotype	加型效应 Additive effect/d	加性效应百分率 Additive effect contribution/%
C32007	RM6324-S1-3	7.34	1	qHD1.1	67.0	-4.6	-6.0	84.6	-10.3	-9.8	Ⅰ
C32009	Lsts1-34-RM11189	7.92	1	qHD1.2	68.3	-4.0	-5.2	85.4	-9.9	-9.4	Ⅰ
C32016	RM11762-S1-15	0.69	1	qHD1.3	66.0	-5.1	-6.7	82.4	-11.4	-10.8	Ⅰ
C32042	RM13034-RM13263	8.67	2	qHD2.2	70.0	-3.3	-4.3	85.8	-9.9	-9.4	Ⅰ
C32050	S2-30-S2-9	1.01	2	qHD2.3	69.3	-3.7	-4.8	84.8	-10.4	-9.8	Ⅰ
C32076	S4-2	2.50	4	qHD4.1	68.2	-2.8	-3.8	85.8	-8.5	-8.3	Ⅰ
C32121	RM21767-S7-17	5.83	7	qHD7.2	69.2	-4.4	-5.8	85.2	-10.9	-10.2	Ⅰ
C32122	S7-5	1.48	7	qHD7.3	66.0	-6.0	-7.9	82.8	-12.1	-11.3	Ⅰ
C32129	RM22722	1.39	8	qHD8.2	70.4	-3.8	-5.0	86.6	-10.2	-9.5	Ⅰ
C32145	RM25213-S10-24	4.44	10	qHD10.1	67.4	-4.4	-5.8	84.8	-10.2	-9.7	Ⅰ
C32146	S10-27-RM25486	3.62	10	qHD10.2	68.2	-4.0	-5.2	84.2	-10.5	-10.0	Ⅰ
C32153	S11-9-ZHsts11-43	7.15	11	qHD11.3	67.4	-4.4	-5.8	84.6	-10.3	-9.8	Ⅰ
C32163	S12-17	0.80	12	qHD12.2	69.6	-3.3	-4.3	86.6	-9.3	-8.8	Ⅰ
C32070	S3-9-S3-10	3.80	3.0	qHD3.3	99.3	12.8	17.3	109.0	3.1	3	Ⅱ
C32102	RM7158-S6-18	1.50	6	qHD6.1	94.2	10.0	13.5	112.2	4.5	4.4	Ⅱ
C32107	RM20069	0.77	6	qHD6.3	95.2	10.5	14.2	117.2	7.0	6.8	Ⅱ
C32034	Zsts2-1-RM12705	3.81	2	qHD2.1	96.4	9.9	12.9	99.6	-3.0	-2.8	Ⅲ
C32086	RM17305-S4-29	3.79	4	qHD4.2	92.9	9.6	12.9	99.2	-1.8	-1.8	Ⅲ
C32113	RM20659-S6-40	2.24	6	qHD6.5	85.3	5.6	7.5	98.8	-2.2	-2.1	Ⅲ
C32124	S7-26-RM22185	0.33	7	qHD7.4	101.7	11.9	15.6	103.4	-1.8	-1.7	Ⅲ
C32126	RM6925-S8-1	2.55	8	qHD8.1	99.0	10.5	13.9	103.2	-1.9	-1.8	Ⅲ
C32020	S1-15-S1-27	3.60	1	qHD1.4	102.1	13.0	17.0	104.2	-	-	Ⅳ
C32053	RM3894	1.02	2	qHD3.1	92.1	7.8	10.1	103.2	-	-	Ⅳ
C32071	S3-11-S3-12	5.22	3	qHD3.4	90.8	8.5	11.5	101.6	-	-	Ⅳ
C32098	S5-15-RM3170	4.26	5	qHD5.2	100.4	13.1	17.7	102.8	-	-	Ⅳ
C32105	S6-21-S6-7	3.06	6	qHD6.2	88.9	7.4	9.9	104.8	-	-	Ⅳ
C32112	RM162-S6-16	3.08	6	qHD6.4	102.2	14.0	18.9	105.4	-	-	Ⅳ
C32115	RM21344-S7-3	6.60	7	qHD7.1	95.0	10.4	14.0	103.2	-	-	Ⅳ
C32130	RM22825-RM22905	4.32	8	qHD8.3	91.7	6.9	9.0	109.2	-	-	Ⅳ
C32133	RM23175-RM23642	8.07	8	qHD8.4	85.5	3.8	4.9	109.0	-	-	Ⅳ
C32140	S9-4	1.54	9	qHD9.1	97.3	9.7	12.7	109.8	-	-	Ⅴ
C32144	RM24805	1.47	9	qHD9.2	99.4	10.7	14.1	109.0	-	-	Ⅳ
C32147	RM26076-S11-4	2.17	11	qHD11.1	86.8	5.3	7.0	105.8	-	-	Ⅳ
C32154	S11-29-S11-31	4.33	11	qHD11.4	88.7	6.3	8.2	104.8	-	-	Ⅳ
C32157	RM6094	0.08	11	qHD11.5	94.3	9.1	11.9	103.2	-	-	Ⅳ
C32158	RM27460-S12-8	0.56	12	qHD12.1	97.1	10.5	13.7	108.2	-	-	Ⅳ
C32061	RM3434-Zsts3-8	7.20	3	qHD3.2	72.3	-	-	87.2	-7.8	-7.6	Ⅴ
C32088	M17605-RM17693	2.51	4	qHD4.3	70.0	-	-	86.4	-8.4	-8.1	Ⅴ
C32091	S5-1	1.70	5	qHD5.1	70.2	-	-	86.6	-8.3	-8.0	Ⅴ
C32149	S11-5-RM26343	3.60	11	qHD11.2	72.4	-	-	84.8	-10.2	-9.7	Ⅴ