Aluminium Tolerance of OsPIN2 Overexpressed Rice Seedlings under Pot Culture

doi:10．3969/j．issn．1001G7216．2015．03．004

Abstract

Abstract:

Differences in Al tolerance of OsPIN2 overexpressed rice seedlings (OXs) and its wild-type (WT) were investigated in 20% and 55% Al saturated soils in a pot experiment. Results indicated that in 55% Al-saturated soil, growth of root and shoot of rice seedlings was inhibited, the first tillering time was delayed, and tiller angle and numbers were reduced. OXs showed less growth inhibition both in roots and shoots than those of WT. More tiller numbers and larger tiller angles were also found in OXs than those in WT. Moreover, the contents of phosphorus, calcium and magnesium in the shoots of OXs were 1.71, 1.25 and 1.32 times of those of WT, respectively. In comparison to WT, OXs accumulated less Al in the roots, root-shoot injunction and stems, and 28.5%-109.8% more Al in the leaves. These results suggest that overexpressing of OsPIN2 in rice seedlings enhance the uptake of phosphorus, calcium and magnesium, reduce Al accumulation in roots and root-shoot injunction, and thus alleviate the toxicity of Al to rice roots and tillering under Al stress.

Key words: rice (Oryza sativa), OsPIN2, pot culture, aluminum stress

摘要：

通过盆栽实验,研究了水稻OsPIN2超表达材料(OXs)及其野生型材料(WT)在铝饱和度为20%和55%土壤中的生长及养分吸收的差异。结果表明,土壤铝胁迫明显抑制水稻根系和地上部的生长,水稻第一次分蘖出现的时间推迟,分蘖角减小,分蘖数减少。与WT相比,OXs的生长受抑制程度较小。在铝饱和度55%的土壤中,OXs茎叶中磷、钙和镁含量分别是WT的1.71、1.25和1.32倍。OXs的根系、根茎连接处和茎的铝累积比WT少,而叶片的铝累积比WT多28.5%~109.8%。这些结果表明,土壤铝胁迫下,OsPIN2超表达能增强水稻对磷、钙、镁的吸收,减少铝在根系和根茎连接处累积而增加其在叶片累积,这样减轻了铝对水稻根系和分蘖的毒害作用。

关键词: 水稻, OsPIN2, 盆栽, 铝胁迫

CLC Number:

Dao-ming WU, Hua-ping CAO, Xiao-li YU, Hong SHEN. Aluminium Tolerance of OsPIN2 Overexpressed Rice Seedlings under Pot Culture[J]. Chinese Journal OF Rice Science, 2015, 29(3): 250-258.

吴道铭, 曹华苹, 于晓莉, 沈宏. 盆栽条件下OsPIN2超表达水稻材料耐铝性研究[J]. 中国水稻科学, 2015, 29(3): 250-258.

Figures/Tables 6

Table 1 The properties of the two soils.

理性性状 Physicochemical property	赤红壤1 Latosolic red soil 1	赤红壤2 Latosolic red soil 2
Al₂(SO₄)₃·16H₂O	0	0.25%
有机质 Organic matter/(g·kg^-1)	8.26±0.73 a	8.60±0.88 a
碱解氮 Available nitrogen/(mg·kg^-1)	75.37±0.82 a	80.62±2.16 a
速效钾 Available potassium/(mg·kg^-1)	14.82±0.43 a	11.71±0.63 a
速效磷 Available phosphorus/(mg·kg^-1)	6.06±0.23 a	7.00±0.29 a
pH值pH value(水∶土= 2.5∶1)	4.87±0.02 a	4.16±0.20 b
铝饱和度 Aluminium saturation/%	20.17±2.72 b	55.38±3.57 a

Fig. 1. Growth of WT, OX1 and OX2 at variaus Al saturation levels.（WT, Wild type; OX1 and OX2,OsPIN2 overexpressed rice seedling. A, Phenotypes of WT, OX1 and OX2. B, Roots, Bar =3 cm. C, Dry weight of shoots. D, Dry weight of roots. Data are means ± SE (n = 4). Different letters above the bars mean significant difference (P<0.05, Tukey test). 22% Al,22% Al saturation; 55% Al,55% Al saturation.The same as in figures below.）

Fig. 2. Effect of Al stress on the tillering of WT, OX1 and OX2 in soil culture.（A, Time of the first tiller, B,Tiller number;C,Tiller angle of WT, OX1 and OX2. Data are means ± SE (n = 4). Different letters above the bars mean significant difference (P<0.05, Tukey test); D, Tillering. Arrows show the first tiller.）

Fig. 3. Plant nitrogen (N), phosphorus (P), and potassium (K) contents as affected by soil Al saturation.（Data are means ± SE (n = 3). Different letters above the bars mean significant difference (P<0.05, Tukey test).）

Fig. 4. Plant calcium (Ca), and magnesium (Mg) contents as affected by soil Al saturation.( Data are means ± SE (n = 3). Different letters above the bars mean significant difference (P<0.05, Tukey test).)

Fig. 5. Aluminum accumulation in different parts of rice plants at various soil Al saturation levels. (A and B, Roots stained with hematoxilin; C, Aluminum accumulation in the roots, D, Aluminum accumulation in root-shoot junctions, E, Aluminum accumulation in stems; F, Aluminum accumulation in leaves. Data are means ± SE (n = 3). Different letters above the bars mean significant difference (P<0.05, Tukey test).)

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