Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (3): 241-249.DOI: 10.3969/j.issn.1001G7216.2015.03.003
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Qing DONG1,2, Ying-xin ZHANG1,2, Zhen-hua ZHANG1,2, Quan ZHOU1,2, Ya-zhi QIN1,2,3, Hong WANG1,2, Shi-hua CHENG1,2, Li-yong CAO1,2,*(), Xi-hong SHEN1,2,*()
Received:
2014-11-27
Revised:
2014-12-24
Online:
2015-05-10
Published:
2015-05-10
Contact:
Li-yong CAO, Xi-hong SHEN
董青1,2, 张迎信1,2, 张振华1,2, 周全1,2, 秦亚芝1,2,3, 王宏1,2, 程式华1,2, 曹立勇1,2,*(), 沈希宏1,2,*()
通讯作者:
曹立勇,沈希宏
基金资助:
CLC Number:
Qing DONG, Ying-xin ZHANG, Zhen-hua ZHANG, Quan ZHOU, Ya-zhi QIN, Hong WANG, Shi-hua CHENG, Li-yong CAO, Xi-hong SHEN. Genetic Analysis and Gene Mapping of a Yellow-green Leaf Mutant w390 in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2015, 29(3): 241-249.
董青, 张迎信, 张振华, 周全, 秦亚芝, 王宏, 程式华, 曹立勇, 沈希宏. 水稻黄绿叶突变体w390的遗传分析和基因定位[J]. 中国水稻科学, 2015, 29(3): 241-249.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001G7216.2015.03.003
光合色素含量 Photosynthetic pigment content | 中恢8015 R8015 | w390 | P |
---|---|---|---|
叶绿素a Chlorophyll a | 2.65±0.03 | 1.34±0.05 | <0.0001 |
叶绿素b Chlorophyll b | 0.74±0.05 | 0.00 | <0.0001 |
类胡萝卜素 Carotenoids | 0.66±0.04 | 0.36±0.01 | <0.0003 |
总含量 Total content | 4.05±0.07 | 1.70±0.06 | <0.0001 |
Table 1 Comparison of photosynthetic pigment contents in leaves between the mutant and its wild-type parent.mg/g
光合色素含量 Photosynthetic pigment content | 中恢8015 R8015 | w390 | P |
---|---|---|---|
叶绿素a Chlorophyll a | 2.65±0.03 | 1.34±0.05 | <0.0001 |
叶绿素b Chlorophyll b | 0.74±0.05 | 0.00 | <0.0001 |
类胡萝卜素 Carotenoids | 0.66±0.04 | 0.36±0.01 | <0.0003 |
总含量 Total content | 4.05±0.07 | 1.70±0.06 | <0.0001 |
性状 Trait | 中恢8015 R8015 | w390 | P |
---|---|---|---|
株高 Plant height/cm | 112.3±5.2 | 98.9±5.5 | 0.0003 |
单株有效穗数 No. of panicles per plant | 8.1±1.4 | 6.3±1.8 | 0.0227 |
每穗总粒数 No. of spikelets per panicle | 118.0±9.1 | 96.2±7.3 | <0.0001 |
每穗实粒数 No. of grains per panicle | 71.1±6.7 | 51.5±5.9 | <0.0001 |
千粒重 1000-grain weight /g | 33.5±0.4 | 31.9±0.5 | 0.3067 |
单株产量 Grain yield per plant /g | 19.3±2.8 | 10.3±1.4 | <0.0001 |
Table 2 Comparison of main agronomic traits between the mutant and its wild-type parent.
性状 Trait | 中恢8015 R8015 | w390 | P |
---|---|---|---|
株高 Plant height/cm | 112.3±5.2 | 98.9±5.5 | 0.0003 |
单株有效穗数 No. of panicles per plant | 8.1±1.4 | 6.3±1.8 | 0.0227 |
每穗总粒数 No. of spikelets per panicle | 118.0±9.1 | 96.2±7.3 | <0.0001 |
每穗实粒数 No. of grains per panicle | 71.1±6.7 | 51.5±5.9 | <0.0001 |
千粒重 1000-grain weight /g | 33.5±0.4 | 31.9±0.5 | 0.3067 |
单株产量 Grain yield per plant /g | 19.3±2.8 | 10.3±1.4 | <0.0001 |
Fig. 2. Comparison of ultrastructures of chloroplasts in the mesophyll cells between the mutant and its wild-type parent.(A and B, Mesophyll cell and chloroplast of wild-type, respectively; C and D, Mesophyll cell and chloroplast of mutant, respectively. N, Nucleus; CP, Chloroplast; G, Grana.)
引物名称 Primer name | 上游引物 Forward primer (5'-3') | 下游引物 Reverse primer (5'-3') | 退火温度 Anealing temperature/℃ |
---|---|---|---|
D2 | CAGCCTCTCTAATTGACTCTC | CCAAGTAGAATGGCCAAATGT | 55 |
D3 | GCATCAAACTATAATAACTGACT | AGGAAAGTAAACAAGGCCTTA | 55 |
D4 | ACACATATGTAGAGTATTATCCG | GCAAGCTGTCTACACGGTT | 55 |
D8 | AACTCTATTGTTTATGGTGG | TAATTCCGAATTTCAGCTCT | 55 |
CAO1-1 | CATGCCTACTTGTGTCACT | ACCGCTCATGTGTACCATC | 55 |
CAO1-2 | GGCGTATTCCAAACCTATTCG | TTAGAATAAAATACGGTGTGCT | 55 |
CAO1-3 | ATTTCCTACTACCCGAAGCTG | GACTATAGTATGCGGTTACCTT | 55 |
CAO1-4 | GATTACAATTGCATCCCGTGA | ATGCCATCCACAAAGATGCTC | 55 |
CAO1-5 | CAGAAGAAGTCCATGCTCCA | CCGGTTCGATATCCAGTATTGC | 55 |
CAO1-6 | AGATGATACTCTAGTTTCCGACA | AATTAGACAAAACCACCCTCG | 60 |
Table 3 Markers used for fine mapping and sequencing.
引物名称 Primer name | 上游引物 Forward primer (5'-3') | 下游引物 Reverse primer (5'-3') | 退火温度 Anealing temperature/℃ |
---|---|---|---|
D2 | CAGCCTCTCTAATTGACTCTC | CCAAGTAGAATGGCCAAATGT | 55 |
D3 | GCATCAAACTATAATAACTGACT | AGGAAAGTAAACAAGGCCTTA | 55 |
D4 | ACACATATGTAGAGTATTATCCG | GCAAGCTGTCTACACGGTT | 55 |
D8 | AACTCTATTGTTTATGGTGG | TAATTCCGAATTTCAGCTCT | 55 |
CAO1-1 | CATGCCTACTTGTGTCACT | ACCGCTCATGTGTACCATC | 55 |
CAO1-2 | GGCGTATTCCAAACCTATTCG | TTAGAATAAAATACGGTGTGCT | 55 |
CAO1-3 | ATTTCCTACTACCCGAAGCTG | GACTATAGTATGCGGTTACCTT | 55 |
CAO1-4 | GATTACAATTGCATCCCGTGA | ATGCCATCCACAAAGATGCTC | 55 |
CAO1-5 | CAGAAGAAGTCCATGCTCCA | CCGGTTCGATATCCAGTATTGC | 55 |
CAO1-6 | AGATGATACTCTAGTTTCCGACA | AATTAGACAAAACCACCCTCG | 60 |
Fig. 3. Fine mapping and positional cloning of the mutant gene.(A, The mutant locus was mapped on the chromosome 10 between RM228 and RM6673. B, The locus was narrowed to a 71.8 kb genomic region between InDel marker D3 and D4. C, Sequence analysis revealed that the mutant carried two nucleotide substitutions in the eighth exon of LOC_Os10g41760, which led to the substitution of leucine and glycine for histidine and glutamic acid, respectively.)
Fig. 4. Comparison of 3D protein structure model of CAO1 between wild-type and the mutant.(A, Wild-type; B, Mutant; C, Superposition of the mutant and its wild-type. The red and dark blue lines represent the structure haboring the mutations in the mutant and its wild-type, respectively. The purple and light blue regions represent the pheophorbide a oxygenase(PaO) domain in the mutant and its wild-type, respectively.)
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