Dissection of Quantitative Trait Loci for Grain Weight and Its Component Traits in the qTGW1.2 Region on Chromosome 1 of Rice

doi:10．3969/j．issn．1001G7216．2015．03．002

Abstract

Abstract:

This paper reports the dissection of three quantitative trait loci(QTLs)for 1000-grain weight (TGW),grain length(GL)and grain width (GW) on the long arm of chromosome 1 in rice. A plant carrying a heterozygous segment extending from RM212 to RM265 was selected from the Zhenshan 97³/Milyang 46 BC₂F₈ population. The resulting BC₂F₉ population was assayed with DNA markers. Two plants were identified,carrying heterozygous segments covering the intervals RM212-RM11787 and RM11787-RM265 which were previously found to harbor qTGW1.2a and qTGW1.2b for TGW,respectively. Two BC₂F₁₀populations were produced,from which three plants with sequential heterozygous regions were identified,respectively. Using the six BC₂F₁₁ populations derived,a QTL for GL was detected in the interval RM11730-RM11800, and the regions for qTGW1.2a and qTGW1.2b were narrowed down to the 580 kb interval RM11730-Wn33304 and 2.0 Mb interval RM11807-RM11885,respectively. In the meantime,five plants with smaller heterozygous regions were selected,from which five BC₂F₁₂ populations were constructed. A QTL for GL was detected in each of the five populations. The additive effect ranged from 0.03 mm to 0.06 mm with the enhancing alleles derived from Milyang 46. Based on comparison of the segregating regions among the five populations,qGL1.2 for GL was delimited to a 372 kb region flanked by RM11781 and Wn34526. This QTL had the additive genetic action mode and may simultaneously affect TGW, GL and GW.

Key words: rice(Oryza sativa L.), quantitative trait locus, grain length, 1000-grain weight, minor effect

摘要：

对水稻第1染色体长臂上控制粒重、粒长和粒宽的3个QTLs进行了剖析。从珍汕97³/密阳46的BC₂F₈群体中筛选到1个在RM212-RM265区间呈杂合的单株,应用DNA标记检测其衍生的BC₂F₉群体,筛选出在RM212-RM11787和RM11787-RM265区间呈杂合的单株各1个,其杂合区间分别覆盖前期定位的千粒重QTL qTGW1.2a和qTGW1.2b。种植2个BC₂F₁₀群体,筛选出杂合区间相互交叠的单株各3个,自交后获得6个BC₂F₁₁群体,在RM11781-RM11800区间检测到了1个控制粒长的QTL,并将qTGW1.2a和qTGW1.2b分别缩小至580 kb的RM11730-Wn33304和2.0 Mb的RM11807-RM11885区间内。同时,筛选出5个杂合区间相互交叠的单株,衍生了5个BC₂F₁₂群体。QTL分析结果表明,每个群体均检测到粒长QTL,加性效应为0.03~0.06 mm,增效等位基因来自密阳46;经比较各个群体的分离区间,将控制粒长的QTL qGL1.2界定在RM11781和Wn34526之间372 kb的区间内;该QTL呈加性作用,可能同时控制粒重、粒长与粒宽。

关键词: 水稻(Oryza sativa L.), 数量性状座位, 粒长, 千粒重, 微效作用

CLC Number:

Lin-lin WANG, Yu-yu CHEN, Liang GUO, Hong-wei ZHANG, Ye-yang FAN, Jie-yun ZHUANG. Dissection of Quantitative Trait Loci for Grain Weight and Its Component Traits in the qTGW1.2 Region on Chromosome 1 of Rice[J]. Chinese Journal OF Rice Science, 2015, 29(3): 232-240.

王琳琳, 陈玉宇, 郭梁, 张宏伟, 樊叶杨, 庄杰云. 水稻第1染色体qTGW1.2区域粒重组分性状QTL的剖析[J]. 中国水稻科学, 2015, 29(3): 232-240.

Figures/Tables 7

References 25

[1]	林鸿宣,闵绍楷,熊振民,等. 应用RFLP图谱定位分析籼稻粒形数量性状基因座位. 中国农业科学,1995,28(4): 1-7.
[2]	邢永忠,谈移芳,徐才国,等. 利用水稻重组自交系群体定位谷粒外观性状的数量性状基因. 植物学报,2001,43(8): 840-845.
[3]	Huang R Y,Jiang L R,Zheng J S,et al.Genetic bases of rice grain shape: So many genes,so little known.Trends Plant Sci, 2013,18(4): 218-226.
[4]	王余龙,姚友礼,李昙云,等. 水稻籽粒品质性状与粒重关系的初步探讨. 作物学报,1995,21(5): 573-578.
[5]	Cai H W,Morishima H.QTL clusters reflect character associations in wild and cultivated rice.Theor Appl Genet, 2002,104(8): 1217-1228.
[6]	Zhuang J Y,Fan Y Y,Rao Z M,et al.Analysis on additive effects and additive-by-additive epistatic effects of QTLs for yield traits in a recombinant inbred line population of rice.Theor Appl Genet, 2002,105(8): 1137-1145.
[7]	Juenger T,Pérez-Pérez J M,Bernal S,et al. Quantitative trait loci mapping of floral and leaf morphology traits in Arabidopsis thaliana: Evidence for modular genetic architecture.Evol Dev, 2005,7(3): 259-271.
[8]	Gu J F,Yin X Y,Struik P C,et al.Using chromosome introgression lines to map quantitative trait loci for photosynthesis parameters in rice (Oryza sativa L.) leaves under drought and well-watered field conditions.J Exp Bot,2012,63(1): 455-469.
[9]	Wang P,Zhou G L,Cui K H,et al.Clustered QTL for source leaf size and yield traits in rice (Oryza sativa L.).Mol Breed, 2012, 29(1): 99-113.
[10]	Fan C C,Xing Y Z,Mao H L,et al.GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice,encodes a putative transmembrane protein.Theor Appl Genet,2006,112(6): 1164-1171.
[11]	Xie X,Jin F,Song M H,et al.Fine mapping of a yield-enhancing QTL cluster associated with transgressive variation in an Oryza sativa × O.rufipogon cross.Theor Appl Genet, 2008,116(5): 613-622
[12]	余守武,杨长登,樊叶杨,等. 水稻第1染色体千粒重QTL的遗传分解. 科学通报, 2008,53(12): 1389-1394.
[13]	余守武,樊叶杨,杨长登,等. 水稻第1染色体短臂粒长和粒宽QTL的精细定位. 中国水稻科学,2008,22(5): 465-471.
[14]	Guo L,Wang K,Chen J,et al.Dissection of two quantitative trait loci for grain weight linked in repulsion on the long arm of chromosome 1 of rice (Oryza sativa L.).Crop J, 2013,1(1): 70-76.
[15]	陈玉宇,朱玉君,张宏伟,等. 水稻第1染色体长臂上微效千粒重QTL qTGW1.2的验证与分解. 作物学报,2014,40(5): 761-768.
[16]	Zheng K L,Huang N,Bennett J,et al.PCR-based marker-assisted selection in rice breeding. IRRI Discussion Paper Series No.12.Los Banos,Phillipines:International Rice Research Institute,1995.
[17]	Chen X,Temnykh S,Xu Y,et al.Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.).Theor Appl Genet, 1997,95(4): 553-567.
[18]	Wang S,Basten C J,Zeng Z B.Windows QTL Cartographer 2.5. Raleigh,NC,USA:Department of Statistics,North Carolina State University,2011.
[19]	SAS Institute Inc.SAS/ STAT User’s Guide. Cary,NC: SAS Institute,1999.
[20]	Xue W,Xing Y,Weng X,et al.Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice.Nat Genet, 2008,40(6): 761-767.
[21]	Ding X,Li X,Xiong L.Evaluation of near-isogenic lines for drought resistance QTL and fine mapping of a locus affecting flag leaf width,spikelet number,and root volume in rice.Theor Appl Genet, 2011,123(5): 815-826.
[22]	Lin S Y,Sasaki T,Yano M.Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Oryza sativa L., using backcross inbred lines.Theor Appl Genet, 1998, 96(8): 977-1003.
[23]	Monforte A J, Tanksley S D.Fine mapping of a quantitative trait locus (QTL) from Lycopersicon hirsutum chromosome 1 affecting fruit characteristics and agronomic traits: Breaking linkage among QTLs affecting different traits and dissection of heterosis for yield.Theor Appl Genet, 2000, 100(3/4): 471-479.
[24]	张宏伟,朱玉君,陈玉宇,等. 水稻千粒重 QTLqTGW1.1的验证. 中国水稻科学, 2014,28(2): 125-131.
[25]	Lu B, Yang C, Xie K, et al.Quantitative trait loci for grain-quality traits across a rice F2 population and backcross inbred lines.Euphytica, 2013, 192(1):25-35.

性状 Trait	群体 Population	单株数 No. of plants	平均值 Mean	标准差 SD	范围 Range	变异系数 CV/%	偏斜度 Skewness	峰度 Kurtosis
千粒重TGW/g	XY1	167	26.18	0.83	24.14~27.93	3.17	-0.48	-0.31
	XY2	336	26.36	0.66	24.35~28.08	2.52	-0.44	0.39
	XY3	345	25.87	1.16	21.09~27.86	4.47	-1.53	3.02
	XZ1	246	25.50	0.72	22.91~27.09	2.82	-0.99	1.53
	XZ2	111	25.04	0.80	22.48~26.83	3.19	-0.84	1.22
	XZ3	109	25.31	0.63	23.74~26.59	2.49	-0.59	-0.06
粒长GL /mm	XY1	167	7.80	0.08	7.56~7.97	1.04	-0.33	-0.15
	XY2	336	7.92	0.15	7.63~8.35	1.85	0.73	0.08
	XY3	345	7.83	0.08	7.59~8.08	1.02	0.35	0.59
	XZ1	246	7.70	0.08	7.41~7.92	1.09	0.06	0.15
	XZ2	111	7.68	0.07	7.52~7.88	0.92	0.25	0.02
	XZ3	109	7.69	0.07	7.44~7.82	0.91	-0.32	0.20
粒宽GW /mm	XY1	167	2.94	0.04	2.80~3.05	1.52	-0.32	0.33
	XY2	336	2.94	0.08	2.81~3.19	2.78	1.12	0.44
	XY3	345	2.94	0.04	2.82~3.10	1.42	0.29	0.89
	XZ1	246	2.87	0.04	2.75~2.99	1.46	-0.14	-0.08
	XZ2	111	2.85	0.04	2.73~2.95	1.54	-0.22	-0.07
	XZ3	109	2.87	0.04	2.76~2.97	1.36	-0.48	0.69
抽穗期HD /d	XY1	167	76.33	1.94	72~82	2.55	0.21	0.08
	XY2	336	76.41	1.86	72~82	2.44	0.02	0.21
	XY3	345	75.22	1.48	72~80	1.96	0.36	-0.10
	XZ1	246	76.37	2.07	72~83	2.71	0.03	0.17
	XZ2	111	75.52	2.25	72~82	2.98	0.44	-0.54
	XZ3	109	76.88	1.54	73~80	2.00	-0.51	0.23

性状 Trait	群体 Population	单株数 No. of plants	平均值 Mean	标准差 SD	范围 Range	变异系数 CV/%	偏斜度 Skewness	峰度 Kurtosis
千粒重TGW/g	XY1	167	26.18	0.83	24.14~27.93	3.17	-0.48	-0.31
	XY2	336	26.36	0.66	24.35~28.08	2.52	-0.44	0.39
	XY3	345	25.87	1.16	21.09~27.86	4.47	-1.53	3.02
	XZ1	246	25.50	0.72	22.91~27.09	2.82	-0.99	1.53
	XZ2	111	25.04	0.80	22.48~26.83	3.19	-0.84	1.22
	XZ3	109	25.31	0.63	23.74~26.59	2.49	-0.59	-0.06
粒长GL /mm	XY1	167	7.80	0.08	7.56~7.97	1.04	-0.33	-0.15
	XY2	336	7.92	0.15	7.63~8.35	1.85	0.73	0.08
	XY3	345	7.83	0.08	7.59~8.08	1.02	0.35	0.59
	XZ1	246	7.70	0.08	7.41~7.92	1.09	0.06	0.15
	XZ2	111	7.68	0.07	7.52~7.88	0.92	0.25	0.02
	XZ3	109	7.69	0.07	7.44~7.82	0.91	-0.32	0.20
粒宽GW /mm	XY1	167	2.94	0.04	2.80~3.05	1.52	-0.32	0.33
	XY2	336	2.94	0.08	2.81~3.19	2.78	1.12	0.44
	XY3	345	2.94	0.04	2.82~3.10	1.42	0.29	0.89
	XZ1	246	2.87	0.04	2.75~2.99	1.46	-0.14	-0.08
	XZ2	111	2.85	0.04	2.73~2.95	1.54	-0.22	-0.07
	XZ3	109	2.87	0.04	2.76~2.97	1.36	-0.48	0.69
抽穗期HD /d	XY1	167	76.33	1.94	72~82	2.55	0.21	0.08
	XY2	336	76.41	1.86	72~82	2.44	0.02	0.21
	XY3	345	75.22	1.48	72~80	1.96	0.36	-0.10
	XZ1	246	76.37	2.07	72~83	2.71	0.03	0.17
	XZ2	111	75.52	2.25	72~82	2.98	0.44	-0.54
	XZ3	109	76.88	1.54	73~80	2.00	-0.51	0.23

性状 Trait	群体 Population	单株数 No. of plants	平均值 Mean	标准差 SD	范围 Range	变异系数 CV/%	偏斜度 Skewness	峰度 Kurtosis
千粒重TGW/g	XY2-1	270	29.41	0.56	28.16~30.96	1.92	0.20	-0.19
	XY2-2	271	29.80	0.62	28.28~31.50	2.07	0.16	-0.39
	XY2-3	239	29.80	0.62	28.10~31.23	2.08	-0.24	-0.25
	XY3-1	281	30.48	0.55	28.90~32.03	1.80	0.11	-0.19
	XY3-2	267	29.85	0.55	28.58~31.28	1.83	0.08	-0.42
粒长GL/mm	XY2-1	270	8.11	0.09	7.92~8.35	1.10	0.14	-0.49
	XY2-2	271	8.16	0.08	7.95~8.40	1.04	0.10	-0.38
	XY2-3	239	8.23	0.08	8.04~8.40	0.95	0.05	-0.47
	XY3-1	281	8.40	0.08	8.14~8.59	1.00	-0.39	0.31
	XY3-2	267	8.28	0.08	8.10~8.50	0.99	0.14	-0.43
粒宽GW/mm	XY2-1	270	3.20	0.03	3.10~3.30	1.08	-0.22	-0.02
	XY2-2	271	3.23	0.04	3.12~3.33	1.10	0.00	0.07
	XY2-3	239	3.24	0.03	3.16~3.33	1.01	0.01	-0.09
	XY3-1	281	3.34	0.03	3.26~3.40	0.81	-0.24	-0.18
	XY3-2	267	3.30	0.03	3.20~3.37	0.86	-0.22	0.04
抽穗期HD/d	XY2-1	270	93.93	1.61	92~101	1.72	1.23	2.06
	XY2-2	271	93.70	1.67	91~101	1.78	1.54	2.89
	XY2-3	239	93.72	1.79	91~101	1.91	1.43	1.73
	XY3-1	281	91.65	1.48	89~100	1.62	1.02	3.64
	XY3-2	267	91.85	1.30	89~97	1.42	0.57	0.76

性状 Trait	群体 Population	单株数 No. of plants	平均值 Mean	标准差 SD	范围 Range	变异系数 CV/%	偏斜度 Skewness	峰度 Kurtosis
千粒重TGW/g	XY2-1	270	29.41	0.56	28.16~30.96	1.92	0.20	-0.19
	XY2-2	271	29.80	0.62	28.28~31.50	2.07	0.16	-0.39
	XY2-3	239	29.80	0.62	28.10~31.23	2.08	-0.24	-0.25
	XY3-1	281	30.48	0.55	28.90~32.03	1.80	0.11	-0.19
	XY3-2	267	29.85	0.55	28.58~31.28	1.83	0.08	-0.42
粒长GL/mm	XY2-1	270	8.11	0.09	7.92~8.35	1.10	0.14	-0.49
	XY2-2	271	8.16	0.08	7.95~8.40	1.04	0.10	-0.38
	XY2-3	239	8.23	0.08	8.04~8.40	0.95	0.05	-0.47
	XY3-1	281	8.40	0.08	8.14~8.59	1.00	-0.39	0.31
	XY3-2	267	8.28	0.08	8.10~8.50	0.99	0.14	-0.43
粒宽GW/mm	XY2-1	270	3.20	0.03	3.10~3.30	1.08	-0.22	-0.02
	XY2-2	271	3.23	0.04	3.12~3.33	1.10	0.00	0.07
	XY2-3	239	3.24	0.03	3.16~3.33	1.01	0.01	-0.09
	XY3-1	281	3.34	0.03	3.26~3.40	0.81	-0.24	-0.18
	XY3-2	267	3.30	0.03	3.20~3.37	0.86	-0.22	0.04
抽穗期HD/d	XY2-1	270	93.93	1.61	92~101	1.72	1.23	2.06
	XY2-2	271	93.70	1.67	91~101	1.78	1.54	2.89
	XY2-3	239	93.72	1.79	91~101	1.91	1.43	1.73
	XY3-1	281	91.65	1.48	89~100	1.62	1.02	3.64
	XY3-2	267	91.85	1.30	89~97	1.42	0.57	0.76

群体 Population	性状 Trait	表型值±标准差 Phenotypic mean±SD			LOD	A	D	D/[A]	R² /%
群体 Population	性状 Trait	ZS97			LOD	A	D	MY46	H
XY1	TGW/g	25.90±0.84 b	26.55±0.75 a	26.16±0.81 b	2.88	0.32	-0.06	-0.20	7.59
	GL/mm	7.80±0.08 a	7.81±0.07 a	7.79±0.08 a	0.36
	GW/mm	2.92±0.05 b	2.96±0.05 a	2.94±0.04 b	3.82	0.02	-0.01	-0.27	9.93
	HD/d	76.60±2.09 a	76.18±1.92 a	76.25±1.88 a	0.27
XY2	TGW/g	26.25±0.61 a	26.41±0.68 a	26.42±0.66 a	0.95
	GL/mm	7.86±0.15 b	7.95±0.14 a	7.94±0.15 a	4.64	0.05	0.03	0.74	6.21
	GW/mm	2.95±0.08 a	2.93±0.08 a	2.95±0.08 a	0.68
	HD/d	76.41±1.77 a	76.47±1.85 a	76.42±1.94 a	0.03
XY3	TGW/g	25.77±1.03 ab	25.68±1.46 b	26.04±0.97 a	1.49
	GL/mm	7.81±0.07 b	7.85±0.08 a	7.83±0.08 b	3.24	0.02	0.00	-0.08	4.21
	GW/mm	2.93±0.04 a	2.94±0.05 a	2.94±0.04 a	0.29
	HD/d	75.37±1.57 a	75.16±1.45 a	75.18±1.44 a	0.25
XZ1	TGW/g	25.31±0.76 b	25.54±0.63 ab	25.57±0.74 a	1.13
	GL/mm	7.69±0.09 a	7.69±0.10 a	7.70±0.08 a	0.18
	GW/mm	2.84±0.04 c	2.89±0.04 a	2.87±0.04 b	9.07	0.02	0.01	0.24	15.70
	HD/d	76.22±2.39 a	76.52±2.37 a	76.29±1.74 a	0.34
XZ2	TGW/g	24.66±0.87 b	25.18±0.77 a	25.17±0.72 a	1.98
	GL/mm	7.68±0.07 a	7.67±0.06 a	7.69±0.08 a	0.34
	GW/mm	2.83±0.04 b	2.87±0.03 a	2.85±0.04 ab	2.58	0.02	0.00	-0.01	10.07
	HD/d	76.00±2.57 a	75.62±2.16 a	75.25±2.12 a	0.48
XZ3	TGW/g	25.12±0.70 a	25.43±0.48 a	25.33±0.65 a	0.81
	GL/mm	7.68±0.06 a	7.70±0.07 a	7.69±0.08 a	0.17
	GW/mm	2.85±0.03 b	2.89±0.04 a	2.87±0.04 a	3.75	0.02	0.01	0.30	14.51
	HD/d	76.85±1.38 a	76.83±1.47 a	76.92±1.67 a	0.02