OsSIZ1 Regulates the Development and Architecture of the Roots under Phosphate Starvation Conditions in Rice

doi:10.3969/j.issn.1001-7216.2015.01.005

Abstract

Abstract:

Plants cope with the low P environment by altering their root architecture. Here, we studied the role of SUMO E3 ligase SIZ1 on the root development and architecture, and the relationship among OsSIZ1, phosphate stress and auxin using ossiz1 mutants. Compared with wild-type (WT), the ossiz1 mutation inhibited the elongation of seminal roots and adventitious roots, promoted lateral roots formation and root hair proliferation. However, the response of the ossiz1 mutation was stronger than WT under Pi deficiency, namely, the trend of root elongation and the increase of the lateral root density and the root hair number of ossiz1 were more obvious relative to WT seedlings. This suggests that OsSIZ1 regulates the root architecture, and the effect is more obvious under Pi deficiency. The total P concentration of shoots and roots in ossiz1 is higher than WT, this suggests that OsSIZ1 negatively regulates phosphorus acquisition and use. And relative expression of OsYUCCA1 and OsPIN1a/1b in ossiz1 roots is markedly up-regulated compared with WT. This suggests that OsSIZ1 negatively regulates the auxin biosynthesis and polar auxin transport, which is weakened under Pi-deficient conditions. Together, these results suggest that OsSIZ1 regulates Pi starvation-induced root architecture remodeling possibly through the control of auxin patterning.

Key words: rice, phosphate, OsSIZ1, root architecture

摘要：

植物应对磷胁迫的方法之一是改变根系的构型。以水稻SUMO化E3连接酶SIZ1突变体ossiz1为供试材料,研究了OsSIZ1在水稻根发育中的作用以及其与磷胁迫、生长素之间的关系。与野生型相比,OsSIZ1抑制ossiz1种子根和不定根的伸长,促进侧根密度的增加和根毛的增多。缺磷时,突变体ossiz1的反应更强烈,即不定根伸长、侧根密度增大和根毛增多的趋势更加明显。说明OsSIZ1参与调控水稻根构型的改变,低磷时效果更明显。ossiz1地上部和地下部的总磷浓度显著高于野生型,说明OsSIZ1在水稻中负调控磷素的吸收利用。定量RT-PCR结果显示,ossiz1中OsYUCCA1和OsPIN1a/1b的相对表达量显著高于野生型,说明OsSIZ1负调控根中生长素的合成与极性运输,并且缺磷时负调控作用减弱。结果表明,SUMO化E3连接酶OsSIZ1调控缺磷条件下根构型的形成,而且这一过程可能是通过调控生长素分布完成的。

关键词: 水稻, 磷, OsSIZ1, 根构型

CLC Number:

Hong-min ZHOU, Hua-dun WANG, Rui SUN, Wen-xia PEI, Xue-neng WU, Yue CAO, Ya-fei SUN, Guo-hua XU, Shu-bin SUN. OsSIZ1 Regulates the Development and Architecture of the Roots under Phosphate Starvation Conditions in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(1): 35-44.

周红敏, 王化敦, 孙瑞, 裴文霞, 吴学能, 曹越, 孙雅菲, 徐国华, 孙淑斌. 水稻SUMO化E3连接酶SIZ1调控缺磷条件下根的发育和根构型形成[J]. 中国水稻科学, 2015, 29(1): 35-44.

Figures/Tables 7

Table 1 Primers used to amplify the OsYUCCA1 and OsPINs cDNA fragments.

基因 Gene	引物序列 Primer sequences (5'-3')
OsActin	F: GGAACTGGTATGGTCAAGGC
	R: AGTCTCATGGATAACCGCAG
OsPIN1a	F: TCATCTGGTCGCTCGTCTGC
	R: CGAACGTCGCCACCTTGTTC
OsPIN1b	F: TGCACCCTAGCATTCTCAGCA
	R: CCCTCCTCCCAAATTCTACTT
OsYUCCA1	F: TCATCGGACGCCCTCAACGTCGC
	R: GGCAGAGCAAGATTATCAGTC
OsPIN2	F:CAACACCTACTCCAGCCTC
	R:TGGACCAGTCAAGAACCTC

Fig.1. Growth performances of seminal roots of ossiz1 and wild type. A, Root phenotype of rice seedlings 7 d after germination, bar=1 cm; B, The root hair proliferation on the seminal roots of rice seedlings 7 d after germination, bar=2 cm; C,D, The length and the growth rate of seminal root of rice seedlings for 7 d after transfer to the nutrient solution. WT, Wild type.

Fig. 2. Root phenotypic analysis of wild type(WT) and ossiz1 seedlings in the Pi-sufficient(+P) and Pi-deficient(-P) solid culture medium. In A, Bar=1 cm; In C, Bar=2 cm.

Fig. 3. Root phenotype of wild type (WT) and ossiz1 seedlings under Pi-sufficient(+P) and Pi-deficient(-P) conditions. In A, Bar=1 cm; In C; Bar=2 cm.

Fig. 4. Statistical analysis of adventitious roots of ossiz1 and its wild type (WT) seedlings under Pi-sufficient (+P) and Pi-deficient (-P) conditions. A,Root scanning; B, Adventitious roots without large lateral root formation in clumps (a, c, e, g) and adventitious roots with large lateral root formation in clumps (b, d, f, h); C,The ratio of adventitious roots with large lateral root formation in clumps to the total adventitious roots.

Fig. 5. Total P concentration in the shoots and roots of ossiz1 and its wild type (WT) seedlings under Pi-sufficient (+P) and Pi-deficient (-P) conditions in hydroponics culture.

Fig. 6. Expression of auxin genes in roots of WT and ossiz1 in hydroponics experiment. A,Relative expression of auxin biosynthesis gene OsYUCCA1 subjected to +P and -P conditions for 2 weeks; B, Relative expression of auxin efflux transporters OsPINs subjected to +P and -P conditions for 2 weeks.

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