Genetic Analysis and Fine Mapping for a Novel split glume Mutant in Rice

doi:10.3969/j.issn.1001-7216.2015.01.003

Abstract

Abstract:

A split glume mutant is from the mutant library of indica variety Zhongxian 3037 radiated by ⁶⁰Co γ-ray. Compared to its wild type, the mutant has not only split glumes, but also characterized by early heading, lower plant height, reduced seed setting rate and degenerated spikelets, which implies that the mutation has a pleiotropic effect. Genetic and molecular analysis indicated that the mutant was controlled by a recessive nuclear gene, temporally designated as split glume 1 (sg1), and then SG1 was fine mapped to a 47-kb DNA region between the STS markers M1-z21 and M1-z27 on 1, where six ORFs in total were predicted. On the basis of the location of SG1, it is deduced that SG1 is a novel gene regulating the rice glume development.

Key words: rice, flower organ, sg1, fine mapping

摘要：

从中籼3037辐射突变体库中获得一个颖花开裂突变体(split glume1, sg1),表型分析发现该突变体不仅颖壳异常,且伴随着生育期提前,植株变矮,颖花退化等现象,结实率也显著降低。遗传分析和分子生物学分析结果表明该突变受1对隐性核基因控制,并最终将其定位在水稻第1染色体上分子标记M1-z21与M1-z27之间,物理距离约为47 kb,这是一种控制颖花发育的新基因,通过预测发现该区段内存在6个开放阅读框。

关键词: 水稻, 花器官, 颖花开裂突变体, 精细定位

CLC Number:

Q343.5
Q755

Sheng-yuan ZENG, Min GUO, Rong-de LI, Sheng-lan SHENG, Hong-bin GONG, Chang-jie YAN. Genetic Analysis and Fine Mapping for a Novel split glume Mutant in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(1): 22-27.

曾生元, 郭旻, 李荣德, 盛生兰, 龚红兵, 严长杰. 水稻新裂颖突变体sg1的遗传分析与基因定位[J]. 中国水稻科学, 2015, 29(1): 22-27.

Figures/Tables 5

References 29

[1]	Coen E S, Meyerowitz E M.The war of the whorls: Genetic interactions controlling flower development.Nature, 1991, 353: 31-37.
[2]	Ma H, dePamphilis C. The ABCs of floral evolution.Cell, 2000, 101: 5-8.
[3]	Thei Ben G.Development of floral organ identity: Stories from the MADS house.Curr Opin Plant Biol, 2001, 4: 75-85.
[4]	Thei Ben G, Saedler H.Plant biology: Floral quartets.Nature, 2001, 409: 469-471.
[5]	Rounsley S D, Ditta G S, Yanofsky M F.Diverse roles for MADS box genes in Arabidopsis development.Plant Cell Online, 1995, 7: 1259-1269.
[6]	Angenent G C, Colombo L.Molecular control of ovule development.Trends Plant Sci, 1996, 1: 228-232.
[7]	Pelaz S, Ditta G S, Baumann E, et al.B and C floral organ identity functions require SEPALLATA MADS-box genes.Nature, 2000, 405: 200-203.
[8]	Yoshida H.Is the lodicule a petal: Molecular evidence?Plant Sci, 2012, 184: 121-128.
[9]	Yun D P, Liang W Q, Dreni L, et al.OsMADS16 genetically interacts with OsMADS3 and OsMADS58 in specifying floral patterning in rice.Mol Plant, 2013, 6: 743-756.
[10]	Furutani I, Sukegawa S, Kyozuka J.Genome-wide analysis of spatial and temporal gene expression in rice panicle development.Plant J, 2006, 46: 503-511.
[11]	Kater M M, Dreni L, Colombo L.Functional conservation of MADS-box factors controlling floral organ identity in rice andArabidopsis. J Exp Bot, 2006, 57: 3433-3444.
[12]	Yamaguchi T, Lee D Y, Miyao A, et al.Functional diversification of the two C-class MADS box genes OsMADS3 and OsMADS58 inOryza sativa. Plant Cell Online, 2006, 18: 15-28.
[13]	Yamaguchi T, Hirano H Y.Function and diversification of MADS-box genes in rice.Sci World J, 2006, 6: 1923-1932.
[14]	Arora R, Agarwal P, Ray S, et al.MADS-box gene family in rice: Genome-wide identification, organization and expression profiling during reproductive development and stress.BMC Genom, 2007, 8: 242.
[15]	Cui R F, Han J K, Zhao S Z, et al.Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa).Plant J, 2010, 61: 767-781.
[16]	Gao X C, Liang W Q, Yin C S, et al.The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development.Plant Physiol, 2010, 153: 728-740.
[17]	Kobayashi K, Yasuno N, Sato Y, et al.Inflorescence meristem identity in rice is specified by overlapping functions of three AP1/FUL-like MADS box genes and PAP2, a SEPALLATA MADS box gene.Plant Cell Online, 2012, 24: 1848-1859.
[18]	Wang K J, Tang D, Hong L L, et al.DEP and AFO regulate reproductive habit in rice.PLoS Genet, 2010, 6: e1000818.
[19]	Jeon J S, Jang S, Lee S, et al.LEAFY HULL STERILE 1 is a homeotic mutation in a rice MADS box gene affecting rice flower development.Plant Cell Online, 2000, 12: 871-884.
[20]	Prasad K, Parameswaran S, Vijayraghavan U.OsMADS1, a rice MADS‐box factor, controls differentiation of specific cell types in the lemma and palea and is an early‐acting regulator of inner floral organs.Plant J, 2005, 43: 915-928.
[21]	Roger S O, Bendich A J. Extraction of DNA from plant tissues. Plant Mol Biol Manual, 1988(A6): 1-10.
[22]	Lander E S, Green P, Abrahamson J, et al.Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.Genomics, 1987, 1: 174-181.
[23]	Sun Q W, Zhou D X.Rice JMJC domain-containing gene JMJ706 encodes H3k9 demethylase required for floral organ development.Proc Natl Acad Sci, 2008, 105: 13679-13684.
[24]	Li H F, Liang W Q, Hu Y, et al.Rice MADS6 interacts with the floral homeotic genes Superwoman1, MADS3, MADS58, MADS13, and drooping leaf in specifying floral organ identities and meristem fate.Plant Cell Online, 2011, 23: 2536-2552.
[25]	Ohmori S, Kimizu M, Sugita M, et al.MOSAIC FLORAL ORGAN 1, an AGL6-like MADS box gene, regulates floral organ identity and meristem fate in rice.Plant Cell Online, 2009, 21: 3008-3025.
[26]	Sang X C, Li Y F, Luo Z K, et al.CHIMERIC FLORAL ORGAN 1, encoding a monocot-specific MADS box protein, regulates floral organ identity in rice.Plant Physiol, 2012, 160: 788-807.
[27]	Li A H, Zhang Y F, Wu X J, et al.DH1, a LOB domain-like protein required for glume formation in rice. Plant Mol Biol, 2008, 66: 491-502.
[28]	Li H G, Xue D W, Gao Z Y, et al.A putative lipase gene EXTRA GLUME 1 regulates both empty-glume fate and spikelet development in rice.Plant J, 2009, 57: 593-605.
[29]	Yuan Z, Gao S, Xue D W, et al.RETARDED PALEA1 controls palea development and floral zygomorphy in rice.Plant Physiol, 2009, 149: 235-244.

性状 Trait	中籼3037 Zhongxian 3037 (Mean±SD)	突变体 sg1 (Mean±SD)	P值 P-value
株高 Plant height/cm	112.7±1.8	85.2±3.4	0.000
穗长 Panicle length/cm	24.4±0.5	23.8±1.3	0.504
每穗粒数 Grain number per panicle	227.6±40.1	145.7±19.3	0.033
结实率 Seed setting rate/%	89.0±0.2	28.0±0.9	0.000

性状 Trait	中籼3037 Zhongxian 3037 (Mean±SD)	突变体 sg1 (Mean±SD)	P值 P-value
株高 Plant height/cm	112.7±1.8	85.2±3.4	0.000
穗长 Panicle length/cm	24.4±0.5	23.8±1.3	0.504
每穗粒数 Grain number per panicle	227.6±40.1	145.7±19.3	0.033
结实率 Seed setting rate/%	89.0±0.2	28.0±0.9	0.000

组合 Combination	F₁	F₂
组合 Combination	F₁	正常表型株数 Normal plants	突变表型株数 Individuals with mutant type	χ²(3:1)
中花11/sg1 Zhonghua 11/sg1	正常 Normal	1705	95	373.4
sg1/越光 sg1/Koshihikari	正常 Normal	789	61	144.0

组合 Combination	F₁	F₂
组合 Combination	F₁	正常表型株数 Normal plants	突变表型株数 Individuals with mutant type	χ²(3:1)
中花11/sg1 Zhonghua 11/sg1	正常 Normal	1705	95	373.4
sg1/越光 sg1/Koshihikari	正常 Normal	789	61	144.0

标记名称 Name	正向引物序列(5’-3’) Forward primer sequence(5’-3’)	反向引物序列(5’-3’) Reverse primer sequence(5’-3’)	产物所在位置 Position	所在克隆 PAC/BAC
d33	TTCACTACAGATACCATAAACC	CTCAGTCACACTTCATCCCTA	12 842-13 102	AP003295
M1-z3	CGTTTGCCGTCCTGTTC	CCGAGGTCTGCCTGATTTA	74 824-74 943	AP003265
M1-z20	TATAGACGTTTCACTGATGG	GGTGGAAGTCAAGAATAATG	34 968-35 112	AP003265
M1-z21	ATCATCATCACGGTTTCA	ACCTGTTCCTTGCCTCA	15 269-15 396	AP003265
M1-z27	CAATCAAGCCCATAACTAC	TGGGTAGTTACACGAGATC	62 113-62 229	AP003265
M1-z28	GGAGCAGCACAAGAAGG	CCAGTTGAGTTGGAGGG	48 595-48 718	AP003265