Identifying the Genetic Basis of Spikelet Fertility Differences Between BTtype ThreeLine japonica Hybrids

doi:10.3969/j.issn.1001-7216.2014.04.006

Chinese Journal of Rice Science ›› 2014, Vol. 28 ›› Issue (4): 377-383.DOI: 10.3969/j.issn.1001-7216.2014.04.006

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Identifying the Genetic Basis of Spikelet Fertility Differences Between BTtype ThreeLine japonica Hybrids

ZHANG Honggen^#, QIAN Kai^#, SUN Yibiao, PEI Yan, FENG Zhiqiang, LIU Xiaoyu, TANG Shuzhu^*, LIANG Guohua, GU Minghong

Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics， Ministry of Education， Yangzhou University， Yangzhou 225009， China；

Received:2013-12-06 Revised:2014-01-10 Online:2014-07-10 Published:2014-07-10
Contact: TANG Shuzhu*,

BT型三系杂交粳稻不同组合间育性差异原因初探

张宏根^#,钱凯^#,孙一标,裴艳,封智蔷,刘晓宇,汤述翥^*,梁国华,顾铭洪

扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室，江苏扬州225009；

通讯作者: 汤述翥*,
基金资助:
国家自然科学基金资助项目（31071384）；江苏省高校自然科学研究项目（13KJ13210008）；江苏省高校优势学科建设工程资助项目。

Abstract

Abstract: In order to understand the genetic basis of spikelet fertility differences between BTtype threeline japonica hybrids, the CMS lines Yujing 6A, 9201A and their corresponding maintainer lines, and the restorer lines Min 120, TR2604 were used to develop the genetic populations for studying the genetic characteristics of BTtype CMS in different hybrid combinations. According to fertility segregation of the plants from the populations of 9201A//9201B/Min 120, Yujing 6A//Yujing 6B/Min 120 and 9201A//9201B/ TR2604, it was concluded that the fertility restoration of 9201A and Yujing 6A was controlled by one fertility restoration(Rf) gene. Then, the Rf genes in Min120, TR2604 were mapped between 18 772 374－18 967 996 bp with the physical distance of 195 kb and 18 817 631－18 967 996 bp with the physical distance of 150 kb on chromosome 10, respectively, indicating that the Rf genes in Min 120, TR2604 might be Rf1a according to the mapping results of Rf1 in former studies. The fertility restoration of Yujing 6A in the population derived from Yujing 6A and TR2604 was also controlled by Rf1a identified by the genotype and phenotype of plants in Yujing 6A/TR2604//TR2604 BC1F2 population, so the sterility of Yujing 6A/TR2604 was induced by hybrid incompatibility between the two parents.

Key words: threeline japonica hybrid, spikelet fertility differences, fertility restoration gene, mapping

摘要： 为了阐明不同BT型杂交粳稻组合间育性差异的原因，利用豫粳6号A、9201A及其相应保持系与恢复系TR2604、闵120来配制遗传组合就BT型不育细胞质育性恢复遗传进行研究。根据9201A//9201B/闵120、9201A//9201B/TR2604及豫粳6号A//豫粳6号B/闵120三个群体中植株的育性分离，推断 9201A、豫粳6号A的育性恢复均由1对恢复基因控制。基因定位结果表明，恢复系闵120中的恢复基因位于第10染色体上18772374－18967996bp间约195 kb物理区间内，TR2604中的恢复基因位于第10染色体18 817 631－18 967 996 bp间约150 kb物理区间内。根据已克隆Rf1基因的信息可知两恢复系带有的恢复基因均为Rf1a。豫粳6号A/TR2604//TR2604 BC1F2群体中植株的Rf1a位点基因型与育性分析结果表明TR2604对豫粳6号A育性恢复由Rf1a控制，豫粳6号A/TR2604不育由双亲间的不亲和性造成。

关键词: 三系杂交水稻, 育性差异, 恢复基因, 基因定位

CLC Number:

ZHANG Honggen#, QIAN Kai#, SUN Yibiao, PEI Yan, FENG Zhiqiang, LIU Xiaoyu, TANG Shuzhu*, LIANG Guohua, GU Minghong. Identifying the Genetic Basis of Spikelet Fertility Differences Between BTtype ThreeLine japonica Hybrids[J]. Chinese Journal of Rice Science, 2014, 28(4): 377-383.

张宏根#,钱凯#,孙一标,裴艳,封智蔷,刘晓宇,汤述翥*,梁国华,顾铭洪. BT型三系杂交粳稻不同组合间育性差异原因初探[J]. 中国水稻科学, 2014, 28(4): 377-383.

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Identifying the Genetic Basis of Spikelet Fertility Differences Between BTtype ThreeLine japonica Hybrids

BT型三系杂交粳稻不同组合间育性差异原因初探

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