Detection of Quantitative Trait Loci for Chalky Traits in japonica Rice

doi:10.3969/j.issn.1001-7216.2009.04.06

Abstract

Abstract: Using an F2 population including 200 individuals derived from a cross between two japonica rice DL115 with large grain and XL005 with small grain, the quantitative trait loci (QTLs) for chalky traits of rice were identified by composite interval mapping using SSR markers. Three chalky traits, i.e. percentage of grains with chalkiness (PGWC), area of chalky endosperm (ACE) and degree of endosperm chalkiness (DEC), exhibited a continuous distribution in F3 lines, indicating that these chalky traits were quantitative traits controlled by multiple genes. Five, two and one QTLs conferring chalky traits were detected on chromosomes 3, 5 and 6, respectively, including three QTLs for PGWC, two QTLs controlling ACE and three QTL conferring DEC. qPGWC3, qACE3b and qDEC3b were located in RM6832-RM411, RM15456-RM6832 and RM6266-RM15456, and explained 43.89%, 18.83% and 19.57% of the observed phenotypic variance for PGWC, ACE, and DEC, respectively. They were major QTLs and new QTLs. Among the detected eight QTLs, qPGWC6 allele was derived from XL005 without chalky grain, the other seven QTL alleles were derived from DL115 with big chalky grain. The gene actions were partially dominant at PGWC and ACE, and additive at DEC.

Key words: japonica rice, chalky trait, microsatellite marker, quantitative trait locus

摘要： 利用大粒粳稻DL115与小粒粳稻XL005杂交获得的F2群体200个单株为作图群体，采用复合区间作图方法，利用SSR标记对稻米垩白性状进行了数量性状基因座(QTL)检测。研究结果表明，稻米垩白粒率、垩白大小和垩白度在F3株系均呈连续分布，表现为由多基因控制的数量性状。检测到与稻米垩白性状相关的QTL 8个，分别位于第3（5个）、第5（2个）和第6（1个）染色体上，包括与垩白粒率有关的QTL 3个，与垩白大小相关的QTL 2个，与垩白度有关的QTL 3个。其中位于第3染色体RM6832-RM411、RM15456-RM6832和RM6266-RM15456区间的qPGWC3、qACE3b和qDEC3b，分别解释垩白粒率、垩白大小和垩白度表型变异的43.89%、18.83%和19.57%，为主效QTL。上述3个主效QTL所在染色体上的位置与前人研究结果均不一致，认为是新的QTL。所检测到的8个QTL中，除qPGWC6的增效等位基因来自无垩白亲本XL005外，其他7个QTL的增效等位基因均来自垩白性状值较大的亲本DL115。垩白粒率和垩白大小基因作用表现为部分显性，垩白度基因作用表现为加性。

关键词: 粳稻, 垩白性状, 微卫星标记, 数量性状基因座

LI Maomao,XU Lei,REN Junfang,CAO Guilan,YU Liqin,KOH Heejong,HE Haohua,HAN Longzhi. Detection of Quantitative Trait Loci for Chalky Traits in japonica Rice [J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2009.04.06 .

黎毛毛,徐磊,任军芳,曹桂兰,余丽琴,高熙宗,贺浩华韩龙植,. 粳稻垩白性状的QTL检测[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2009.04.06 .

References

[1]张玉华, 张国民, 包秀艳. 水稻垩白米的危害与防治措施. 黑龙江农业科学, 2002(4): 44-46.

[2]矫江, 王伯伦. 我国东北地区稻米垩白发生规律研究. 作物学报, 2003, 29(2): 311-314.

[3]黄农荣, 钟旭华. 早籼稻垩白形成与灌浆期耐热性关系研究初报. 广东农业科学, 2004(1): 6-8.

[4]贾志宽, 高如嵩, 张嵩午, 等. 中国稻米垩白形成的地域分异规律之初步研究. 中国水稻科学, 1992, 6(4): 159-164.

[5]杨仁崔, 梁康迳, 陈青华. 稻米垩白直感遗传和杂交稻垩白米遗传分析. 福建农学院学报, 1986, 15(1): 51-54.

[6]Shi C H, Wu J G, Lou X B. Genetic analysis of transparency and chalkiness area at different filling stages of rice (Oryza sativa L.). Field Crops Res, 2002, 76: 1-9.

[7]Li Z F, Wan J M, Xia J F, et al. Mapping quantitative trait loci underlying appearance quality of rice grains (Oryza sativa L.). Acta Genet Sin, 2003, 30(3): 251-259.

[8]吴顺, 李晓方, 萧浪涛, 等. 稻米垩白与直链淀粉含量的数量性状位点及其相互关系研究. 华南师范大学学报:自然科学版, 2005(1): 90-94.

[9]Wan X Y, Wan J M, Wang J F, et al. Stability of QTLs for rice grain dimension and endosperm chalkiness characteristics across eight environments. Theor Appl Genet, 2005, 110: 1334-1346.

[10]Tan Y F, Xing Y Z, Li J X, et al. Genetic bases of appearance quality of rice grain in Shanyou 63, an elite rice hybrid. Theor Appl Genet, 2000, 101: 823-829.

[11]何平, 李仕贵, 李晶, 等. 影响稻米品质几个性状的基因座位分析. 科学通报, 1998, 43(16): 1747-1750.

[12]韩龙植. 水稻种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006: 6970.

[13]Edwards K, Johnstone C, Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl Acids Res, 1991, 19: 1349.

[14]Basten C J, Weir B S, Zeng Z B. QTL Cartographer, Version 2.5. Raleigh, NC: Department of Statistics, North Carolina State University, 2005.

[15]Manly K F, Cudmore R H, Meer J M. Map Manager QTX, crossplatform software for genetic mapping. Mamm Genome 2001, 12: 930-932.

[16]McCouch S R, Cho Y G, Yano M, et al. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11-13.

[17]Stuber C W, Lincoln S E, Wolff D W, et al. Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics, 1992, 132(6): 823-839.

[18]廖伏明, 周坤炉, 阳和华, 等. 籼型杂交水稻米质性状配合力及遗传力研究. 湖南农业大学学报:自然科学版, 2000, 26(5): 50-54.

[19]张佩莲, 钟旭华, 曾宪江, 等. 穗上不同部位的稻米垩白度差异的研究. 江西农业大学学报, 1995, 17(4): 396-399.

[20]陈秉发, 陈建民, 黄荣裕, 等. 稻米垩白遗传及无垩白稻谷单粒筛选技术初探. 福建农业学报, 2000, 15(4): 1-5.

[21]张坚勇, 肖应辉, 万向元, 等. 水稻品种外观品质性状稳定性分析. 作物学报, 2004, 30（6）: 548-555.

[22]李欣, 汤述翥, 印志同, 等. 粳型杂种稻米品质性状的表现及遗传控制. 作物学报, 2000, 26(4): 411-419.

[23]Koh H J, Heu M H. Agronomic characteristics of a mutant for genic male sterilitychalky endosperm and its utilization on F1 hybrid breeding system in rice. Korean J Crop Sci, 1996, 40(6): 684-696.

[24]Koh H J, Son Y H, Heu M H, et al. Molecular mapping of a new genic malesterility gene causing chalky endosperm in rice (Oryza sativa L.). Euphytica, 1999, 106: 57-62.

[25]Mikami L, Aikawa M, Hirano H Y, et al. Altered tissuespecific expression at the Wx gene of the opaque mutants in rice. Euphytica, 1999, 105: 91-97.