Effects of Nitrogen Forms on Root Morphology and Phosphate Uptake in Rice

Abstract

Abstract: A hydroponic experiment was conducted to study the effect of N forms (NH4+,NO3- and NH4NO3) on root morphology and P uptake in rice. The results indicated that the root morphology of low P starved rice was affected very significantly by N forms. In comparison with ammonium as sole N source, the ammonium nitrate nutrition increased root length, root surface area, root density, and total P uptake. In comparison to either ammonium or nitrate alone, mixture supply of the two forms of N increased transport of P from roots to the aboveground parts. It was suggested that the improved P uptake by mixture supply of both ammonium and nitrate were partially due to the stimulation of root growth.

Key words: ammonium, nitrate, phosphate, root morphology, rice

摘要： 采用水培方法，研究了缺磷条件下不同形态氮（NH₄⁺、NO^₃-和NH₄NO₃）下的水稻根系形态性状，以及它们产生的后效应对磷吸收的影响。结果表明，在氮素供应充足但磷饥饿胁迫的状况下，氮素形态对根系的影响仍然十分显著。与单一的铵营养相比，铵硝混合营养增加了根长和根系的表面积、根系的密度以及磷的总吸收量；与单一的铵或硝营养相比，铵硝混合营养可增加吸收的磷从根系向地上部的运输。因此，铵硝混合营养改善低磷胁迫下水稻对磷的吸收和转运，其部分原因与氮素形态与根系形态发生有关。

关键词: 铵态氮, 硝态氮, 磷素, 根系形态, 水稻

LI Baozhen ,WANG Songwei ,FENG Huimin ,XU Guohua . Effects of Nitrogen Forms on Root Morphology and Phosphate Uptake in Rice [J]. Chinese Journal of Rice Science.

李宝珍王松伟, 冯慧敏,徐国华,. 氮素供应形态对水稻根系形态和磷吸收的影响[J]. 中国水稻科学.

References

[1]Vance C P, UhdeStone C, Allan D L. Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource. New Phytologist, 2003, 157: 423-447.

[2]Rufty T W J R, Israel D W, Volk R J, et al. Phosphate regulation of nitrate assimilation in soybean. J Exp Bot, 1993, 44: 879-891.

[3]Markus L, Alberto S, Peter S, et al. Root development of maize (Zea mays L.) as observed with Minirhizotrons in Lysimeters. Crop Sci, 2000, 40: 1665-1672.

[4]史正军, 樊小林. 作物对氮素养分高效吸收的根系形态学研究进展. 广西农业生物科学, 2003, 22(3)： 225-229.

[5]Li H B, Xia M, Wu P. Effect of phosphorus deficiency stress on rice lateral root growth and nutrient absorption. Acta Bot Sin, 2001, 43(11): 1154-1160.

[6]贾彦博, 杨肖娥, 刘建祥. 植物根系对养分缺乏和毒害的适应及其与养分吸收效率的关系. 土壤通报, 2005, 36(4): 610-616.

[7]李永夫, 罗安程, 黄继德, 等. 不同磷效率水稻基因型根系形态和生理特性的研究. 浙江大学学报:农业与生命科学版, 2006, 32(6): 658-664.

[8]樊剑波, 张亚丽, 万小羽, 等. 水稻根系与氮素吸收利用之研究进展. 中国农学通报, 2007, 23(2): 236-240.

[9]Miller M H. Effects of nitrogen on phosphorous absorption by plants//Carson E W. The Plant Root and Its Environment. Charlottesville, VA: University Press of Virginia, 1974： 643-668.

[10]Smith F W, Jackson W A. Nitrogen enhancement of phosphate transport in roots of Zea mays L.: Ⅰ. Effects of ammonium and nitrate pretreatment. Plant Physiol, 1987, 84: 1314-1318.

[11]Smith F W, Jackson W A. Nitrogen enhancement of phosphate transport in roots of Zea mays L.: Ⅱ. Kinetic and inhibitor studies. Plant Physiol, 1987, 84: 1319-1324.

[12]Cole C V, Grunes D L, Porter L K, et al. The effects of nitrogen on short term phosphorous absorption and translocation in corn (Zea mays L.). Soil Sci Soc Am J, 1963, 27: 671-674.

[13]Thien S J, Mcfee W W. Effect of nitrogen on phosphorous transport systems in Zea mays L. Soil Sci Soc Am J, 1972, 36: 617-620.

[14]鲍士旦. 土壤农化分析. 3版. 北京:中国农业出版社, 1999.

[15]Li B Z, Xin W J, Sun S B, et al. Physiological and molecular responses of nitrogenstarved rice plants to resupply of different nitrogen sources. Plant & Soil, 2006, 287: 145-159.

[16]Lynch J P. Root architecture and plant productivity. Plant Physiol, 1995, 109: 7-13.

[17]乔云发, 苗淑杰, 韩晓增. 氮素形态对大豆根系形态性状及释放H+的影响. 大豆科学， 2006(3)： 265-269.

[18]Linkohr B I, Williamson L C, Fitter A H, et al. Nitrate and phosphate availability and distribution have different effects on root system architecture of Arabidopsis. Plant J, 2002, 29: 751-760.

[19]Buwalda F, Warmenhoven M. Growthlimiting phosphate nutrition suppresses nitrate accumulation in greenhouse lettuce. J Exp Bot, 1999, 50: 813-821.

[20]de Groot C C, Marcelis L F M, van den Boogaard R, et al. Interaction of nitrogen and phosphorus nutrition in determining growth. Plant & Soil, 2003, 248: 257-268.

[21]Martin A C, del Pozo J C, Iglesias J, et al. Influence of cytokinins on the expression of phosphate starvation responsive genes in Arabidopsis. Plant J, 2000, 24: 559-567.