Genetic Analysis and Gene Mapping of a Twisted Hull Mutant in Rice

Chinese Journal of Rice Science

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Genetic Analysis and Gene Mapping of a Twisted Hull Mutant in Rice

LI Jinbo¹, XIA Mingyuan ¹, WAN Bingliang ¹, DU Xueshu ¹, ZHA Zhongping 1, YU Dazhao ², QI Huaxiong ^{1, *}

¹Food Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; ² Plant Protection and Soil and Fertilizer Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; *Corresponding author, E-mail: qihx@hbaas.com

Received:1900-01-01 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10

一个水稻颖壳扭曲突变体的遗传分析与基因定位

李进波¹,夏明元¹,万丙良¹,杜雪树¹,查中萍¹,喻大昭² ,戚华雄^{1, *}

¹湖北省农业科学院粮食作物研究所，湖北武汉 430064； ²湖北省农业科学院植保土肥研究所，湖北武汉 430064；*通讯联系人， E-mail: qihx@hbaas.com

Abstract

Abstract: A twisted hull rice mutant was found in the progeny of a breeding population. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive gene (temporarily designated as Osth). To map OsTH(t) gene, an F2 population was established by crossing the Osth mutant with R725, an indica rice variety with normal hulls. Finally, OsTH(t) was located between the SSR markers RM14128 and RM208 on the long arm of chromosome 2 at the genetic distances of 1.4 cM and 2.7 cM, respectively. These results provides the foundation for further fine mapping, cloning and functional analysis of OsTH(t) gene.

Key words: Oryza sativa, twisted hull mutant, genetic analysis, gene mapping

摘要： 从水稻育种后代材料中获得1个颖壳扭曲突变体Osth (twisted hull)。遗传分析结果表明，该突变性状由单核基因隐性突变造成。以突变体与颖壳正常籼稻R725杂交的F2群体为基因定位群体，利用SSR标记将突变位点定位在第2染色体上的SSR标记RM14128与RM208之间，遗传距离分别为1.4 cM 和2.7 cM。这些结果为该基因的精细定位和克隆以及研究水稻花发育的分子机理奠定了基础。

关键词: 水稻, 颖壳扭曲突变体, 遗传分析, 基因定位

LI Jinbo ,XIA Mingyuan ,WAN Bingliang ,DU Xueshu ,ZHA Zhongping ,YU Dazhao ,QI Huaxiong . Genetic Analysis and Gene Mapping of a Twisted Hull Mutant in Rice[J]. Chinese Journal of Rice Science.

李进波,夏明元,万丙良,杜雪树,查中萍,喻大昭 ,戚华雄, . 一个水稻颖壳扭曲突变体的遗传分析与基因定位[J]. 中国水稻科学.

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Genetic Analysis and Gene Mapping of a Twisted Hull Mutant in Rice

一个水稻颖壳扭曲突变体的遗传分析与基因定位

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