Co-segregation Analysis Between TDNA Tag and Character of Small Grain Dwarf Mutant in Rice

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Co-segregation Analysis Between TDNA Tag and Character of Small Grain Dwarf Mutant in Rice

MA Yuyin^1,3, ZHANG Yafang¹, PAN Cunhong¹, LI Aihong¹, WU Xujiang¹, ZUO Shimin¹, CHEN Zongxiang¹, WU Changyin², PAN Xuebiao^1,*

¹Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genetics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; ²National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 3Department of Biochemistry, Yangzhou College of Education, Yangzhou 225002, China; *Corresponding author, E-ail： shuidao@yzu.edu.cn

Received:1900-01-01 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10

水稻小粒矮突变性状与T-DNA插入标签的共分离检测

马玉银1^{, 3},张亚芳¹,潘存红¹,李爱宏¹,吴旭江¹,左示敏¹,陈宗祥¹,吴昌银²,潘学彪¹,*

¹扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室，江苏扬州 225009； ²华中农业大学作物遗传改良国家重点实验室，湖北武汉 430070； ³扬州教育学院生物化学系，江苏扬州 225002； *通讯联系人， E-ail： shuidao@yzu.edu.cn

Abstract

Abstract: A small grain dwarf (sgd) mutant was identified in the segregated population from a TDNAtag rice line. Genetic analysis of this mutant was conducted for cloning and functional research of the gene. Multiple TDNA insertion copies in this line were confirmed, and one of them had been homozygous in T3 segregation population. As a result, it was impossible to detect cosegregation between the tag and sgd merely depended on the specific PCR primer from TDNA in T3 and successively generations. However, a flanking sequence from one of TDNA insertion sites was obtained by TAILPCR, and was confirmed to be cosegregated with sgd in consecutively segregated generations.

Key words: T-NA tagging technology, flanking sequence, cosegregation, rice, small grain dwarf mutant

摘要： 从T-DNA水稻标签系中发现1个标签系中出现了小粒矮(sgd)突变性状的分离。为克隆该突变基因和进行基因功能研究，对该突变体进行了遗传分析。该标签系存在多个插入事件，且T3代发现目标突变性状分离系中，另一个非目标插入事件已经纯合，故特异PCR检测不能确认小粒矮突变性状与T-NA插入共分离。但通过TAIL-CR方法，获得了插入点侧翼的水稻基因组序列，并证实该侧翼序列与小粒矮突变性状共分离。

关键词: T-NA标签技术, 侧翼序列, 共分离, 水稻, 小粒矮突变

MA Yuyin,ZHANG Yafang,PAN Cunhong,LI Aihong,WU Xujiang,ZUO Shimin,CHEN Zongxiang,WU Changyin,PAN Xuebiao . Co-segregation Analysis Between TDNA Tag and Character of Small Grain Dwarf Mutant in Rice [J]. Chinese Journal of Rice Science.

马玉银张亚芳,潘存红,李爱宏,吴旭江,左示敏,陈宗祥,吴昌银,潘学彪,. 水稻小粒矮突变性状与T-DNA插入标签的共分离检测[J]. 中国水稻科学.

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