Development of Molecular Markers for Sbe Gene in Association with Starch Characteristic in Rice

Abstract

Abstract: Based upon the genomic sequence difference between the indica rice variety 9311 and the japonica rice variety Nipponbare, a total of nine molecular markers for starch branching enzyme gene(Sbe) were successfully developed. All the markers were utilized for genotypic screening and further analysis of the correlation between genetic polymorphisms of Sbe1 and Sbe3 genes and the rice starch characteristics. The genetic polymorphism of the markers had no influence on starch physiochemical traits, that is these allelomorphism variation sites of Sbe gene had no influence on the starch traits of nonglutinous rice. There were six markers which could be used to accurately identify indica rice varieties with a reliability over 80%. Meaning of marker development and molecular marker assisted selection were also discussed.

Key words: rice, starch branching enzyme gene, molecular marker, starch viscosity, variety identification

摘要： 基于籼稻9311和粳稻日本晴基因组间的序列差异，成功发展了水稻淀粉分支酶基因(Sbe)9个分子标记。利用这些标记对102份非糯材料进行了基因型检测，并分析了Sbe1、Sbe3基因标记基因型多态性对稻米淀粉理化特性的影响。Sbe1、Sbe3基因标记多态性位点对揭示非糯材料间稻米淀粉理化特性的差异不显著，说明淀粉分支酶基因这些等位性变异位点对非糯稻米的蒸煮食味品质影响不显著；在鉴定籼稻品种时，有6个标记的鉴定准确率达80%以上。还对标记开发的意义和分子标记辅助选择育种进行了探讨。

关键词: 水稻, 淀粉分支酶基因, 分子标记, 淀粉黏滞性

LI Gang,#,DENG Qiming,#,WAN Yingxiu,LI Shuang cheng,XIAO Yong,ZHOU Peng,WANG Shiquan,LI Ping .

^{Development of Molecular Markers for Sbe Gene in Association with Starch Characteristic in Rice}

[J]. Chinese Journal of Rice Science.

李刚,邓其明,万映秀,李双成肖勇周鹏王世全李平. 稻米淀粉理化特性相关的水稻淀粉分支酶基因标记开发[J]. 中国水稻科学.

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