Abstract: To establish a cheaper EcoTILLING system based on agarose-gel-electrophoresis for the detection of natural variation of rice or other plant species,a convenient EcoTILLING technique combined agarose gel electrophoresis with active rude CELⅠ nuclease extract from celery was constructed. By using the EcoTILLING technique,two SNPs were detected in a Yunnan conventional rice variety Dadiaonuo.

Key words: targeting induced local lesions in genome, agarose gel electrophoresis, CEL Ⅰ nuclease, mutation detection, single nucleotide polymorphism

摘要： 为构建低成本的基于琼脂糖凝胶电泳的EcoTILLING技术体系，用于水稻及其他生物自然群体变异的检测，对基于琼脂糖凝胶的EcoTILLING技术进行了优化。结果表明，从芹菜中提取的CEL Ⅰ核酸酶粗提物结合琼脂糖凝胶电泳检测技术，可以成功地检测到目的基因位点的突变。利用该技术，在云南地方水稻品种大吊糯中检测到2 个SNPs。

关键词: 定向诱导基因组局部突变, 琼脂糖电泳, CEL Ⅰ核酸酶, 突变检测, 单核苷酸多戊性