Abstract: Northern blot analysis showed that the expression levels of the rice gene OsSABATH were up-regulated by infestation of rice striped stem borer,Chilo suppressalis,but not by mechanical wounding. A 2050-bp promoter sequence upstream of this gene was amplified using a pair of primers designed on the basis of the sequence of the OsSABATH and rice genome sequence database. With the aid of PLACE program,several cis-acting elements in the promoter sequence,including W-box,DOF binding motif,GT-1 element,and BIHD1 binding motif,were recognized. For analyzing the activity of the promoter sequence and the functions of the herbivore feeding responsive cis-acting elements,the promoter region sequence and its 5'deletion constructs were fused to the GUS reporter gene in pCAMBIA1391 vector.

Key words: rice, Chilo suppressalis, promoter, cis-acting element, cloning

摘要： Northern杂交结果表明，OsSABATH基因受二化螟(Chilo suppressalis)为害诱导表达，但却不受机械损伤诱导。利用该基因序列和水稻基因组数据库相应序列设计引物，扩增到该基因编码区5′上游2050 bp的启动子序列。用PLACE软件分析顺式作用元件，发现该基因起始密码子上游1.4 kb的区域内存在几个可能与该基因表达调控相关的顺式作用元件，如W盒、DOF 结合序列、GT1、 BIHD1 结合序列等。将该基因启动子区域及其5′部分缺失构件与GUS报告基因相连，并克隆进pCAMBIA1391载体中，用于分析启动子活性及虫伤诱导特异性相关的顺式作用元件。

关键词: 水稻, 二化螟, 启动子, 顺式作用元件, 克隆