Abstract: The fertility, formation and development of mature embryo sacs in the hybrids between Oryza sativa subsp. japonica cv. Taichung 65 and Oryza rufipogon indigenous to Gaozhou, Guangdong Province, China were studied by the whole-mount eosin B staining confocal laser scanning microscopy (WE-CLSM). The results showed that the average embryo sac fertility was 64.61%, ranging from 20.00% to 97.00%. The abnormal embryo sac types consisted of the following categories: embryo sac degeneration, abnormal small embryo sac, embryo sac without female germ unit, embryo sac without egg apparatus, embryo sac with abnormal polar nuclei number or with polar nuclei located on an abnormal position and other abnormal types. The frequency of embryo sac without female germ unit (10.49%) was the highest among various abnormal types, followed by embryo sac degeneration (9.80%). The embryo sac formation and development of the F1 hybrids was identical with that of the O. satiza, which conforms to the polygonum type. Some abnormalities were observed during embryo sac development in F1 hybrids with low embryo sac fertility, The embryo sac of three hybrids were observed at 1 day after pollination (DAP) By WE-CLSM, it was found that some embryo sacs with normal structure were unfertilized and some abnormal embryo sacs were also unfertilized. The embryo sacs of three hybrids at 7 DAP were similar to those at 1 DAP.

Key words: Oryza sativa, Oryza rufipogon, interspecific hybrid, embryo sac, fertility, development, whole-mount eosin B staining confocal laser scanning microscopy

摘要： 利用整体染色激光扫描共聚焦显微术(WECLSM), 对广东高州普通野生稻与粳稻台中65杂交F1的成熟胚囊育性、胚囊形成发育及受精特点进行研究。杂种F1成熟胚囊平均育性为64.61%。不同杂种的成熟胚囊均存在不同程度的异常现象, 异常的主要类型有：胚囊退化、小胚囊、雌性生殖单位退化、卵器退化、极核数目或位置异常及其他异常等类型。其中频率最高的异常类型为雌性生殖单位退化（10.49%），其次是胚囊退化（9.80%）。杂种F1的胚囊发育过程与高州野生稻原种基本一致，育性低的杂种F1胚囊发育过程出现多种异常现象。对3个杂种F1受粉后1 d的胚囊进行观察发现，除正常受精膨大的胚囊外，还存在结构正常未受精和结构异常未受精等异常现象。受粉后7 d的胚囊基本上与受粉后1 d的相似。研究结果表明，广东高州普通野生稻可能具有决定杂种F1胚囊育性的不同基因，包括中性基因等。

关键词: 栽培稻, 普通野生稻, 种间杂种, 胚囊, 育性, 发育, 整体染色激光扫描共聚焦显微术