Abstract: By using fluorescence differential display and RT-PCR methods, a new member of the calmodulin-binding proteins family of rice was isolated and termed as OsCaMBP.The full length cDNA was 2094 bp and encoded a polypeptide containing 569 amino acid residues (63.2 kD). Amino acid sequence of the deduced protein revealed the existence of IQ calmodulin-binding motif at the N-terminus. Similarity analysis indicated that rice OsCaMBP has 38.25%-47.28% identities with CaMBPs in other plants. Expression of the OsCaMBP gene was greatly increased in leaves, roots and leaf sheaths after the heat shock treatment at 42℃ for 15min, 30min, 1h and 2h. Furthermore, the gene expression accumulation was enhanced in response to chilling stress.

Key words: rice, calmodulin-binding protein, fluorescence differential display, gene cloning, expression

摘要： 通过荧光差异显示和RTPCR技术,克隆到一个新的水稻钙调素结合蛋白基因OsCaMBP。序列分析表明，该基因cDNA序列全长2094 bp，编码一个具有569个氨基酸残基的多肽，推测分子量为63.2 kD；在OsCaMBP蛋白的N端存在IQ钙调素结合构象，与其他植物中的钙调素结合蛋白的相似性介于38.25%~47.28%。在热激处理15 min、30 min、 1 h或2 h后，该基因在水稻的叶、根和叶鞘中表达量急剧增加；此外，该基因表达量也受低温调控而增加。

关键词: 水稻, 钙调素结合蛋白, 荧光差异显示, 基因克隆, 表达