Abstract: The protein expression profile during rice-Xanthomonas oryzae pv. oryzae (Xoo) interaction by using a two dimensional eleccrophoresis-mass spectrometry strategy was analyzed. Proteins were extracted from rice leaves at 1, 4 , 8, 48, and 72 hours after Xoo inoculation. Seventy two differentially expressed protein spots were identified by comparing the expression profiles of proteins from Xoo and mock-inoculated plants revealed by two-dimensional electrophoresis. Further analysis using electrospray ionization time of flight mass spectrometry revealed the propercy of 11 of the 72 differentially expressed proteins. These results provide basis for further studying the molecular mechanism of rice disease resistance.

Key words: rice, disease resistance, proteomics, Xanthomonas oryzae pv. oryzae, expression profile

摘要： 采用双向电泳质谱分析技术，比较分析了水稻在接种白叶枯病菌 （Xanthomonas oryzae pv. oryzae）1、4、8、24、72 h 后蛋白质表达谱的差异。通过比较接种白叶枯病菌和对照之间、接种不同白叶枯病菌生理小种之间的表达差异，鉴定了72个差异表达的抗病相关蛋白点，对其中部分点的蛋白质进行了电离子喷雾二级质谱学分析鉴定，确定了11个抗病反应中差异表达的蛋白质。

关键词: 水稻, 抗病性, 蛋白质组学, 白叶枯病菌, 表达谱