Abstract: By using autotetraploid polyembryonic mutant rice IR36-Shuang as material,the process and characters of its double fertilization,embryo and endosperm development were observed with a laser scanning confocal microscopy. Some abnormalities including degradated ovary,abortive embryo sac,single fertilization,double-ovule and double-embryo were found during the double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67%,significantly higher than that in the control IR36-4x(33.00%). Cytological and embryonic proofs were provided for seed setting decline and the initiation of additional embryo in the IR36-Shuang.

Key words: autotetraploid polyembryonic rice, double fertilization, reproductive developmental character, developmental biology, laser scanning confocal microscopy

摘要： 以同源四倍体多胚苗突变水稻IR36双为材料，利用激光扫描共聚焦显微镜技术对其双受精过程及胚、胚乳的早期发育特征进行了观察。在“IR36双”双受精及胚胎发育过程中，观察到一些异常受精现象和胚囊发生败育的异常类型。统计结果表明，异常受精作用或败育胚囊的发生频率高达46.67%，远高于其野生型品种IR364x（33.00%）。主要有子房退化、胚囊退化、单受精、双胚珠、双胚等几种异常类型。这些异常类型是导致结实率降低及形成双胚苗的细胞学基础。

关键词: 同源四倍体多胚苗水稻, 双受精, 生殖发育特征, 发育生物学, 激光扫描共聚焦显微术