Abstract: Recently, the epidemic of rice black-streaked dwarf disease caused by rice black-streaked dwarf virus or southern rice black-streaked dwarf virus has caused great yield losses in rice production in South China and East China. However, the occurrence of this disease in Hubei Province, China has not been reported. In 2009 and 2010, a rice dwarf disease, similar to rice black-streaked dwarf disease, caused great rice yield losses in some counties of Hubei Province along the Yangtze River. By symptom observation, dsRNA profiling and RT-PCR, the pathogen of the rice dwarf disease was identified as southern rice black-streaked dwarf virus(SRBSDV). This indicates that SRBSDV has been spread from the south to the central rice-growing area of China by the insect vector Sogatella furcifera.

Key words: rice black-streaked dwarf virus, southern rice black-streaked dwarf virus, genome electrophoresis, reverse transcription polymerase chain reaction, virus identification

摘要： 近年来由水稻黑条矮缩病毒和南方水稻黑条矮缩病毒引起的水稻黑条矮缩病在华南、华东等地区暴发流行，给水稻生产带来严重损失，而湖北一直未有水稻黑条矮缩病流行的报道。然而，2009年和2010年在湖北省长江沿线的荆州、孝感、咸宁、黄石、鄂州、黄冈等水稻种植区普遍发生水稻矮缩减产的现象。经过症状观察、dsRNA电泳图谱分析、RT-PCR等方法，诊断该病是由南方水稻黑条矮缩病毒引起，说明该病毒已经随介体昆虫白背飞虱从我国南方传播至中部水稻种植区。

关键词: 水稻黑条矮缩病毒, 南方水稻黑条矮缩病毒, 基因组电泳, 反转录聚合酶链式反应, 病毒鉴定