Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses

doi:10.3969/j.issn.1001-7216.2010.06.002

Abstract

Abstract: Plant calcineurin Blike (CBL) proteins have been proposed as important Ca2+ sensors and specifically interact with CBLinteracting protein kinases (CIPKs) in plantspecific calcium signaling. We identified and isolated 15 CIPK genes in japonica rice variety Nipponbare based on the predicted sequences of rice CIPK gene family. Gene structure analysis showed that these 15 genes were divided into intronless and intronrich groups, in which OsCIPK3 and OsCIPK24 exhibit alternative splicing. The phylogenetic analysis indicated that rice CIPKs share an ancestor with Arabidopsis and poplar CIPKs. Analysis of gene expression showed that these OsCIPK genes were differentially induced by biotic stress (bacterial blight, BB) and abiotic stresses, including heavy metal (Hg2+), high salt, cold and ABA. Interestingly, five OsCIPK genes (OsCIPK1, OsCIPK2, OsCIPK10, OsCIPK11 and OsCIPK12) were transcriptionally upregulated after BB infection, among which four OsCIPK genes (OsCIPK2, OsCIPK10, OsCIPK11 and OsCIPK14) were induced by all treatments, indicating that some of OsCIPKs were involved in multiple stress response pathways in plants. The findings suggest that CIPKs play a key role in both biotic and abiotic stress responses.

Key words: rice, CIPK family, gene expression, stress, bacterial blight

摘要： 类钙调磷酸酶B亚基蛋白（calcineurin Blike proteins，CBL）是植物细胞中重要的Ca2+传感器，必须与蛋白激酶CIPK（CBLinteracting protein kinases）特异性互作才能发挥生物学功能。根据水稻OsCIPK（Oryza sativa CIPK）基因家族的预测序列，从粳稻日本晴（Nipponbare）中克隆了15个OsCIPK基因。基因结构分析表明15个OsCIPK可归为多内含子和少内含子两类，OsCIPK3和OsCIPK24的mRNA存在可变剪接。系统进化树分析显示水稻CIPK家族与拟南芥、杨树来源于同一个祖先。这15个OsCIPK在不同程度上受生物胁迫（白叶枯病）和非生物胁迫（Hg2+、高盐、冷和ABA）的诱导表达；其中，5个基因（OsCIPK1、OsCIPK2、OsCIPK10、OsCIPK11和OsCIPK12）在接种白叶枯病菌后表达显著上调；而4个基因（OsCIPK2、OsCIPK10、OsCIPK11和OsCIPK14）的表达受所有胁迫处理诱导，表明这些基因可能参与多种逆境信号的转导。揭示了CIPK可能在植物的生物和非生物胁迫应答中都具有重要的作用。

关键词: 水稻, CIPK家族, 基因表达, 胁迫, 白叶枯病

CHEN Xi-feng,GU Zhi-min,LIU Feng,MA Bo-jun,ZHANG Hong-sheng. Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2010.06.002 .

陈析丰,顾志敏,刘峰,马伯军,张红生,. 生物与非生物胁迫下水稻CIPK基因的鉴定分析 [J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2010.06.002 .

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